Proliferation of Skeletal Muscle Satellite Cells after Castration and Administration of Testosterone Propionate

Mulvaney, D. R.; Marple, D. N.; Merkel, R. A.

doi:10.3181/00379727-188-42704

Cited by 39 publications

(25 citation statements)

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“…Satellite cells are direct targets of androgen action upregulating AR concentration and decreasing differentiation of porcine satellite cells in culture (20,35). Testosterone also enhances satellite cell activity and myonuclei incorporation into growing muscle fibers of the triceps brachii from postnatal pigs (43). The rat levator ani muscle also has increased satellite cell activation and myonuclei accumulation with testosterone treatment (35).…”

Section: Discussionmentioning

confidence: 99%

Steroid receptor concentration in aged rat hindlimb muscle: effect of anabolic steroid administration

Carson

Lee

McClung

et al. 2002

Journal of Applied Physiology

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receptor concentration in aged rat hindlimb muscle: effect of anabolic steroid administration. J Appl Physiol 93: 242-250, 2002; 10.1152/japplphysiol.01212.2001.-Skeletal muscle is a target of anabolic steroid action; however, anabolic steroid's affect on aged skeletal muscle is not well understood. The effect of 4 wk of nandrolone decanoate (ND) administration on hindlimb muscles of 5-and 25-mo-old Fischer 344/Brown Norway rats was examined. ND (6 mg/kg body wt) was injected every 7th day for 4 wk. Controls received an oil injection. ND significantly reduced 25-mo-old rat perirenal fat pad mass by 30%. Soleus (Sol) and plantaris (Plan) muscle-to-body weight ratios were reduced in 25-mo-old rats. ND did not affect Sol or Plan muscle-to-body weight ratios at either age. Sol DNA concentration was reduced by 25% in 25-mo-old rats, and ND increased it to 12% greater than 5-mo-old rats. ND did not affect Plan DNA content. Sol androgen receptor (AR) protein in 25-mo-old rats was reduced to 35% of 5-mo-old values. ND increased AR protein by 900% in 25-mo-old rat Sol. Plan AR concentration was not affected by aging but was induced by ND in both age groups. Aging or ND treatment did not affect glucocorticoid receptor levels in either muscle. These data demonstrate that fast-and slow-twitch rat hindlimb muscles differ in their response to aging and ND therapy.

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Section: Discussionmentioning

confidence: 99%

Steroid receptor concentration in aged rat hindlimb muscle: effect of anabolic steroid administration

Carson

Lee

McClung

et al. 2002

Journal of Applied Physiology

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“…Different studies have suggested that androgens act on satellite cells or myoblasts to increase the number of myonuclei (Mulvaney et al 1988, Joubert et al 1994, Joubert & Tobin 1995 or act on myofibers to increase protein content (Sheffield-Moore et al 1999, Ferrando et al 2002, Chen et al 2008, Ophoff et al 2009b. However, despite the fact that the AR is deleted by ≥93% in hind-limb muscles of both mAR ΔZF2 lines in our study, muscle mass is virtually normal with only relative muscle mass decreased in α-actin mAR ΔZF2 TA and soleus, but all other hind-limb muscles not different to AR lox controls.…”

Section: Discussionmentioning

confidence: 99%

Muscle-specific androgen receptor deletion shows limited actions in myoblasts but not in myofibers in different muscles in vivo

Rana¹,

Chiu²,

Russell³

et al. 2016

Journal of Molecular Endocrinology

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The aim of this study was to investigate the direct muscle cell-mediated actions of androgens by comparing two different mouse lines. The cre-loxP system was used to delete the DNA-binding activity of the androgen receptor (AR) in mature myofibers (MCK mAR ΔZF2 ) in one model and the DNA-binding activity of the AR in both proliferating myoblasts and myofibers (α-actin mAR ΔZF2 ) in another model. We found that hind-limb muscle mass was normal in MCK mAR ΔZF2 mice and that relative mass of only some hind-limb muscles was reduced in α-actin mAR ΔZF2 mice. This suggests that myoblasts and myofibers are not the major cellular targets mediating the anabolic actions of androgens on male muscle during growth and development. Levator ani muscle mass was decreased in both mouse lines, demonstrating that there is a myofiber-specific effect in this unique androgen-dependent muscle. We found that the pattern of expression of genes including c-myc, Fzd4 and Igf2 is associated with androgen-dependent changes in muscle mass; therefore, these genes are likely to be mediators of anabolic actions of androgens. Further research is required to identify the major targets of androgen actions in muscle, which are likely to include indirect actions via other tissues.

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“…In search of this mitogen, it was found that the proliferation and differentiation of satellite cell-derived myoblasts cultured in vitro respond to various growth factors in a dose-dependent manner (10,11). Numerous studies further revealed that the proliferation and differentiation of satellite cells during muscle regeneration is profoundly influenced by innervation, the vasculature, hormones, nutrition, and the extent of tissue injury (121,224,250,332,360,410). Interestingly, satellite cells in contact with the plasma membrane of intact myofibers were found to have reduced sensitivity to mitogen stimulation (50).…”

Section: A a Brief History Of Satellite Cellsmentioning

confidence: 99%

Satellite Cells and the Muscle Stem Cell Niche

Yin

Price

Rudnicki

2013

Physiological Reviews

1,715

1,808

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LYin H, Price F, Rudnicki MA. Satellite Cells and the Muscle Stem Cell Niche. Physiol Rev 93: 23-67, 2013; doi:10.1152/physrev.00043.2011.-Adult skeletal muscle in mammals is a stable tissue under normal circumstances but has remarkable ability to repair after injury. Skeletal muscle regeneration is a highly orchestrated process involving the activation of various cellular and molecular responses. As skeletal muscle stem cells, satellite cells play an indispensible role in this process. The self-renewing proliferation of satellite cells not only maintains the stem cell population but also provides numerous myogenic cells, which proliferate, differentiate, fuse, and lead to new myofiber formation and reconstitution of a functional contractile apparatus. The complex behavior of satellite cells during skeletal muscle regeneration is tightly regulated through the dynamic interplay between intrinsic factors within satellite cells and extrinsic factors constituting the muscle stem cell niche/microenvironment. For the last half century, the advance of molecular biology, cell biology, and genetics has greatly improved our understanding of skeletal muscle biology. Here, we review some recent advances, with focuses on functions of satellite cells and their niche during the process of skeletal muscle regeneration.

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Proliferation of Skeletal Muscle Satellite Cells after Castration and Administration of Testosterone Propionate

Abstract: Thirty-six neonatal pigs were randomly assigned to the following treatment groups: sham implanted gonadally intact males (B), sham-implanted castrated males (C), or castrated

Cited by 39 publications

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Steroid receptor concentration in aged rat hindlimb muscle: effect of anabolic steroid administration

Steroid receptor concentration in aged rat hindlimb muscle: effect of anabolic steroid administration

Muscle-specific androgen receptor deletion shows limited actions in myoblasts but not in myofibers in different muscles in vivo

Satellite Cells and the Muscle Stem Cell Niche

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