Proliferation of mouse embryonic stem cells on substrate coated with intact silkworm sericin

Umehara, Chihiro; Lian, Ai Ai; Funahashi, Yuichiro; Takaki, Keiko; Maruta, Rina; Ohmaru, Yuto; Okahisa, Yoko; Aoki, Takashi; Mori, Hajime; Kotani, Eiji

doi:10.1080/00405000.2021.1986282

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…Fibroin from degummed cocoons likely loses its random-coiled structure and forms a β-sheet conformation after protein degradation. A previous study suggested that β-sheet structures weaken stem cell interactions with sericin-coated scaffolds and enhance cell-cell interactions [38].…”

Section: Discussionmentioning

confidence: 99%

Bioengineered Silkworm for Producing Cocoons with High Fibroin Content for Regenerated Fibroin Biomaterial-Based Applications

Yamano

Hirose

Lye

et al. 2022

IJMS

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Silk fibroin exhibits high biocompatibility and biodegradability, making it a versatile biomaterial for medical applications. However, contaminated silkworm-derived substances in remnant sericin from the filature and degumming process can result in undesired immune reactions and silk allergy, limiting the widespread use of fibroin. Here, we established transgenic silkworms with modified middle silk glands, in which sericin expression was repressed by the ectopic expression of cabbage butterfly-derived cytotoxin pierisin-1A, to produce cocoons composed solely of fibroin. Intact, nondegraded fibroin can be prepared from the transgenic cocoons without the need for sericin removal by the filature and degumming steps that cause fibroin degradation. A wide-angle X-ray diffraction analysis revealed low crystallinity in the transgenic cocoons. However, nondegraded fibroin obtained from transgenic cocoons enabled the formation of fibroin sponges with varying densities by using 1–5% (v/v) alcohol. The effective chondrogenic differentiation of ATDC5 cells was induced following their cultivation on substrates coated with intact fibroin. Our results showed that intact, allergen-free fibroin can be obtained from transgenic cocoons without the need for sericin removal, providing a method to produce fibroin-based materials with high biocompatibility for biomedical uses.

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Section: Discussionmentioning

confidence: 99%

Bioengineered Silkworm for Producing Cocoons with High Fibroin Content for Regenerated Fibroin Biomaterial-Based Applications

Yamano

Hirose

Lye

et al. 2022

IJMS

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“…Additionally, fibroblast growth factor-2 has been loaded onto regenerated sericin-based biomaterials used for the treatment of bone defects [ 10 ]. Nonetheless, sericin extracted from ordinary cocoons is usually fragmented and accompanied by the loss of some important biochemical properties [ 20 , 21 ]. Furthermore, the production, purification, and handling of labile proteins such as growth factors would constitute a significant portion of the final product cost.…”

Section: Introductionmentioning

confidence: 99%

A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes

Lian

Yamaji

Kajiwara

et al. 2022

IJMS

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Growth factors, including fibroblast growth factor-7 (FGF-7), are a group of proteins that stimulate various cellular processes and are often used with carriers to prevent the rapid loss of their activities. Sericin with great biocompatibility has been investigated as a proteinaceous carrier to enhance the stability of incorporated proteins. The difficulties in obtaining intact sericin from silkworm cocoons and the handling of growth factors with poor stability necessitate an efficient technique to incorporate the protein into a sericin-based biomaterial. Here, we report the generation of a transgenic silkworm line simultaneously expressing and incorporating FGF-7 into cocoon shells containing almost exclusively sericin. Growth-factor-functionalized sericin cocoon shells requiring simple lyophilization and pulverization processes were successfully used to induce the proliferation and migration of keratinocytes. Moreover, FGF-7 incorporated into sericin-cocoon powder exhibited remarkable stability, with more than 70% of bioactivity being retained after being stored as a suspension at 25 °C for 3 months. Transgenic sericin-cocoon powder was used to continuously supply biologically active FGF-7 to generate a three-dimensionally cultured keratinocyte model in vitro. The outcomes of this study propound a feasible approach to producing cytokine-functionalized sericin materials that are ready to use for cell cultivation.

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Proliferation of mouse embryonic stem cells on substrate coated with intact silkworm sericin

Cited by 2 publications

References 30 publications

Bioengineered Silkworm for Producing Cocoons with High Fibroin Content for Regenerated Fibroin Biomaterial-Based Applications

Bioengineered Silkworm for Producing Cocoons with High Fibroin Content for Regenerated Fibroin Biomaterial-Based Applications

A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes

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