Proliferation of Lacrimal Gland Acinar Cells in Primary Culture. Stimulation by Extracellular Matrix, EGF, and DHT

Schönthal, Axel H.; Warren, Dwight W.; Stevenson, Douglas; Schecter, Joel E; Azzarolo, Ana Maria; Mircheff, Austin K.; Trousdale, Melvin D.

doi:10.1006/exer.2000.0824

Cited by 38 publications

(20 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many recent studies have noted the bene®t of seeding preparations of lacrimal acinar cells onto Matrigel-coated culture plates (Kelleher et al, 1991;Meneray et al, 1994;Millar et al, 1996;Chen et al, 1998). Recent studies by the authors group have established procedures for the isolation of highly puri®ed preparations of lacrimal acinar cells (Guo et al, 2000) and methods which enhance the proliferation of the lacrimal acinar cells (Scho Ènthal et al, 2000). Prior to this time lacrimal acinar cells could be established and maintained in culture for short periods of time, approximately 7 days, with minimal proliferation.…”

Section: Introductionmentioning

confidence: 96%

“…The most recent methods (Scho Ènthal et al, 2000) combine the bene®ts of using Hepato Stim 1 Medium [supplemented with epidermal growth factor (EGF) and fetal bovine serum (FBS)] and Matrigel, a solubilized preparation known to be rich in extracellular matrix molecules derived from the basement membranes of the Engelbreth±Holm±Swarm (EHS) mouse sarcoma line (Kleinman et al, 1982). Unlike past studies in which Matrigel was used to coat the surfaces of culture plates, the authors have devised methods to coat the preparations of puri®ed, suspended, lacrimal acinar cells with Matrigel.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Growth of Purified Lacrimal Acinar Cells in Matrigel Raft Cultures

Schechter

Stevenson

Chang

et al. 2002

Experimental Eye Research

Self Cite

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Growth of Purified Lacrimal Acinar Cells in Matrigel Raft Cultures

Schechter

Stevenson

Chang

et al. 2002

Experimental Eye Research

Self Cite

View full text Add to dashboard Cite

“…Previous studies showed that the maintenance of the morphology and secretory function of lacrimal gland cells in vitro critically depends on the use of an extracellular matrix and on the composition of growth factors and nutrients in the culture medium [7,16,20,21,23,26].…”

Section: Introductionmentioning

confidence: 99%

Amniotic membrane as a carrier for lacrimal gland acinar cells

Schrader

Wedel

Kremling

et al. 2007

Graefes Arch Clin Exp Ophthalmol

View full text Add to dashboard Cite

show abstract

“…The rate of EGF secretion into the tear fluid rapidly increases in response to stimulation (van Setten, 1990;Yoshino et al, 1997), and lacrimal EGF mRNA expression increases in response to corneal injury (Wilson et al, 1999). EGF stimulates proliferation of lacrimal acinar cells in primary culture (Schönthal et al, 2000). EGF also has been reported to increase protein secretion by acinar cells from rat lacrimal glands (Dartt, 2001;Tepavcevic et al, 2003).…”

mentioning

confidence: 98%

Novel biphasic traffic of endocytosed EGF to recycling and degradative compartments in lacrimal gland acinar cells

Xie

Wang

et al. 2003

Journal Cellular Physiology

View full text Add to dashboard Cite

The purpose of this study was to delineate the traffic patterns of EGF and EGF receptors (EGFR) in primary cultured acinar epithelial cells from rabbit lacrimal glands. Uptake of [(125)I]-EGF exhibited saturable and non-saturable, temperature-dependent components, suggesting both receptor-mediated and fluid phase endocytosis. Accumulation of [(125)I] was time-dependent over a 120-min period, but the content of intact [(125)I]-EGF decreased after reaching a maximum at 20 min. Analytical fractionation by sorbitol density gradient centrifugation and phase partitioning indicated that within 20 min at 37 degrees C [(125)I] reached an early endosome, basal-lateral recycling endosome, pre-lysosome, and lysosome. Small components of the label also appeared to reach the Golgi complex and trans-Golgi network. Intact [(125)I]-EGF initially accumulated in the recycling endosome; the content in the recycling endosome subsequently decreased, and by 120 min increased amounts of [(125)I]-labeled degradation products appeared in the pre-lysosomes and lysosomes. Confocal microscopy imaging of FITC-EGF and LysoTrackerRed revealed FITC enriched in a dispersed system of non-acidic compartments at 20 min and in acidic compartments at 120 min. Both confocal immunofluorescence microscopy and analytical fractionation indicated that the intracellular EGFR pool was much larger than the plasma membrane-expressed pool at all times. Cells loaded with [(125)I]-EGF released a mixture of intact EGF and [(125)I]-labeled degradation products. The observations indicate that in lacrimal acinar cells, EGFR and EGF-EGFR complexes continually traffic between the plasma membranes and a system of endomembrane compartments; EGF-stimulation generates time-dependent signals that initially decrease, then increase, EGF-EGFR traffic to degradative compartments.

show abstract

Proliferation of Lacrimal Gland Acinar Cells in Primary Culture. Stimulation by Extracellular Matrix, EGF, and DHT

Cited by 38 publications

References 30 publications

Growth of Purified Lacrimal Acinar Cells in Matrigel Raft Cultures

Growth of Purified Lacrimal Acinar Cells in Matrigel Raft Cultures

Amniotic membrane as a carrier for lacrimal gland acinar cells

Novel biphasic traffic of endocytosed EGF to recycling and degradative compartments in lacrimal gland acinar cells

Contact Info

Product

Resources

About