Proliferation of human lung cancer in an orthotopic transplantation mouse model

Abstract

Abstract. the objective of this study was to clarify the growth and proliferation style of human lung cancer grown in an orthotopic transplantation model. the human lung squamous cell carcinoma SQ5 and adenocarcinoma a549 cell lines were used. tumor cells suspended in serum-free medium were directly injected into the main bronchi of anesthetized female Balb/c athymic nude mice with simultaneous administration of 0.01 m ethylenediaminetetraacetic acid. Bromodeoxyuridine was injected into mice 20 min before sacrifice. Lung tissue with tumor nodules and subcutaneous tumors were fixed and confirmed by histological examinations. Bromodeoxyuridinelabeled cells in the tumor area were counted, and the proliferation index was calculated. lung tumor colonies of various sizes were obtained in the SQ5-and a549-cell orthotopically transplanted mice. Orthotopic SQ5 tumors whose minor diameter was 40-700 µm and major diameter was 80-830 µm showed no definite necrosis. Orthotopic SQ5 tumors whose minor diameter was 540-5,200 µm and major diameter was 600-6,100 µm showed definite necrosis in the tumor center. Similar results were also found in the orthotopic a549 tumors. the proliferation index was 7.38 (3.03)/10.63 (3.10) in the orthotopic SQ5 tumors with/without necrosis and 6.99 (2.10) in the subcutaneous SQ5 tumors with necrosis, respectively. the proliferation index was 2.70 (0.88)/3.53 (1.70) in the orthotopic a549 tumors with/without necrosis and 3.91 (0.63) in the subcutaneous a549 tumors with necrosis, respectively. the data suggest that this orthotopic transplantation model may provide the proper organ microenvironment for lung cancer growth and may be suitable for the target therapy research of human lung cancer.

Longitudinal Assessment of Lung Cancer Progression in Mice Using the Sodium Iodide Symporter Reporter Gene and SPECT/CT Imaging

“…Furthermore, animal models can recapitulate human disease and may provide insight into certain aspects of disease pathology, progression, and treatment. However, with respect to lung cancer, no reliable animal model currently exists [32]. …”

“…These in vivo studies yielded clear images of hNIS-expressing tumor xenografts using 99m TcO 4 - . Here, we translated Chen and colleagues’ work to an orthotopic lung cancer transplantation mouse model, similar to that developed by Kang et al, which simulated the clinical features of human lung cancer [39,32]. …”

Editorial for “Intravoxel Incoherent Motion Diffusion‐Weighted Imaging for Predicting and Monitoring the Response of Anti‐Angiogenic Treatment in the Orthotopic Nude Mouse Model of Lung Adenocarcinoma”

“…The authors overcame the challenges associated with image distortion due to magnetic heterogeneity and motion artifacts 5 . This is an animal study, but they used orthotopic implantation tumors in the lungs 6 . The IVIM‐DWI can demonstrate tumors in the rat lung, and IVIM parameters can be analyzed.…”

“…5 This is an animal study, but they used orthotopic implantation tumors in the lungs. 6 The IVIM-DWI can demonstrate tumors in the rat lung, and IVIM parameters can be analyzed. Therefore, this result is promising for application to clinical situations.…”

Improvement of orthotopic lung cancer mouse model via thoracotomy and orotracheal intubation enabling in vivo imaging studies

“…Orthotopic tumor models, on the other hand, are better representations for testing the efficacy of various anti-cancer agents when performed under more practical and clinically applicable conditions providing better preclinical predications of therapeutics than do SC tumor models. 6,7 However, orthotopic tumor models of small animals can be difficult to create due to the size limitations of their anatomies. In particular, mice have narrow glottis openings which act as obstacles to intubation, complicating orthotopic lung tumor model creation.…”