Proliferation of chondrocytes on porous poly(dl-lactide)/chitosan scaffolds

Wu, Hua; Wan, Ying; Cao, Xiaoying; Wu, Qingyin

doi:10.1016/j.actbio.2007.06.010

Cited by 61 publications

(46 citation statements)

References 55 publications

(51 reference statements)

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“…Solvent casting/salt leaching [13], freeze-drying [14,15] and gas foaming [16] have been used to generate porosity in chitosan and composite mixtures of chitosan and other polymers. The disadvantages of these techniques include the use of toxic solvents, the removal of solvent by evaporation (which takes days or even weeks), labour-intensive processing, irregularly shaped pores, insufficient interconnectivity and the inability to culture cells throughout the hydrogel scaffold [17].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of porous chitosan scaffolds for soft tissue engineering using dense gas CO2

et al. 2011

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Section: Introductionmentioning

confidence: 99%

Fabrication of porous chitosan scaffolds for soft tissue engineering using dense gas CO2

et al. 2011

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“…An increase in the experimental days resulted in the subsequent increase in the cell proliferation. [45,46] The extent of increase in the cell proliferation was highest in F3 and lowest in F1. Interestingly, at longer time durations, the cell proliferation in F3 and F4 was found to be similar ( Figure 5(b)).…”

Section: Proliferation Assaymentioning

confidence: 96%

Evaluation of poly(L-lactide) and chitosan composite scaffolds for cartilage tissue regeneration

Mallick

Pal

Rastogi

et al. 2016

Designed Monomers and Polymers

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The present study delineates the development of chitosan and poly(L-lactide) (PLLA) scaffolds cross-linked using a mixture of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), n-hydroxysuccinimide (NHS), and chondroitin sulfate (CS) for cartilage tissue engineering applications. Chitosan and PLLA were varied in concentration for developing scaffolds and prepared by freeze-drying method. The various scaffolds were studied using scanning electron microscopy (SEM), porosity by mercury intrusion porosimeter, and the molecular interactions among polymers using Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) studies. Differential scanning calorimetry (DSC) was used to predict the thermal properties of the scaffolds. The mechanical properties of the scaffolds were studied using static mechanical tester. The ability of the scaffolds to support chondrocyte proliferation was also studied. The microscopy suggests that the pore size of the scaffolds varied with the composition in the range of 38-172 μm and the porosities in the range of 73-93%. The XRD and the FTIR studies suggested that an alternation in the composition of the scaffolds altered the molecular interactions among the scaffold components. An increase in the chitosan content enhanced the swelling property. The degradation of the scaffolds was least when the proportion of chitosan and PLLA was in the ratio of 70:30. The in vitro cell proliferation study suggested that the developed scaffolds were able to support chondrogenesis, the glycosaminoglycan (GAG) content of the mature chondrocyte was 40 μg/ml and the viability was approximately 90%. Hence, the so designed scaffolds may be tried for cartilage tissue engineering applications.

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“…In general, two approaches to the formation of TEHV can be distinguished by the nature of the scaffolding material: (1) bioresorbable polymer scaffold, and (2) decellularized valve scaffold [1] . Although elaborate manufacturing techniques using stereolithographic anatomical models have been used for polymeric heart valve scaffolds, this approach so far could not reproduce the functional complex 3-dimensional composite heart valve architecture and has limitations in cellular adhesion [2,3] . The use of a decellularized valve scaffold offers the advantages of its innate anatomical architecture and remains the active sites for cell adhesion and growth factors, but its mechanical strength is decreased [4][5][6][7] .…”

mentioning

confidence: 99%

Fabrication of a novel hybrid scaffold for tissue engineered heart valve

Hong

Dong

Shi

et al. 2009

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The aim of this study was to fabricate biomatrix/polymer hybrid scaffolds using an electrospinning technique. Then tissue engineered heart valves were engineered by seeding mesenchymal stromal cells (MSCs) onto the scaffolds. The effects of the hybrid scaffolds on the proliferation of seed cells, formation of extracellular matrix and mechanical properties of tissue engineered heart valves were investigated. MSCs were obtained from rats. Porcine aortic heart valves were decellularized, coated with poly(3-hydroxybutyrate-co-4-hydroxybutyrate) using an electrospinning technique, and reseeded and cultured over a time period of 14 days. In control group, the decellularized valve scaffolds were reseeded and cultured over an equivalent time period. Specimens of each group were examined histologically (hematoxylin-eosin [HE] staining, immunohistostaining, and scanning electron microscopy), biochemically (DNA and 4-hydroxyproline) and mechanically. The results showed that recellularization was comparable to the specimens of hybrid scaffolds and controls. The specimens of hybrid scaffolds and controls revealed comparable amounts of cell mass and 4-hydroxyproline (P>0.05). However, the specimens of hybrid scaffolds showed a significant increase in mechanical strength, compared to the controls (P<0.05). This study demonstrated the superiority of the hybrid scaffolds to increase the mechanical strength of tissue engineered heart valves. And compared to the decellularized valve scaffolds, the hybrid scaffolds showed similar effects on the proliferation of MSCs and formation of extracellular matrix. It was believed that the hybrid scaffolds could be used for the construction of tissue engineered heart valves.

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Proliferation of chondrocytes on porous poly(dl-lactide)/chitosan scaffolds

Cited by 61 publications

References 55 publications

Fabrication of porous chitosan scaffolds for soft tissue engineering using dense gas CO2

Fabrication of porous chitosan scaffolds for soft tissue engineering using dense gas CO2

Evaluation of poly(L-lactide) and chitosan composite scaffolds for cartilage tissue regeneration

Fabrication of a novel hybrid scaffold for tissue engineered heart valve

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