Proliferation, migration, and invasion of human glioma cells exposed to fractionated radiotherapy in vitro

Abstract

Radiotherapy is a well established treatment for malignant gliomas. This study describes the migration, proliferation, and invasion behaviour of two human glioma cell lines (GaMg and U-87 Mg) grown as multicellular tumour spheroids after radiotherapy. Migration and proliferation studies were performed using conventional and accelerated fractionation up to 60 Gy and 59.4 Gy, respectively. A dose-dependent growth and migratory response to irradiation independent of the type of fractionation was observed. A coculture system in which tumour spheroids were confronted with foetal rat brain aggregates was used for invasion studies. Marked invasion of the glioma spheroids into the brain aggregates occurred with or without radiotherapy. For the GaMg cells, flow cytometric DNA histograms after treatment with 10 Gy and 40 Gy showed an accumulation of cells in the G2/M phase of the cell cycle. Radiotherapy inhibits tumour cell growth and migration, but the invasiveness of the remaining tumour cells seems to be unaffected.

Radiation sterilisation of cultured human brain tumour cells for clinical immune tumour therapy

“…In a recently published study, however, two human cell lines (GaMG and U-87MG) were irradiated up to 60 Gy. Their results indicate that irradiated malignant glioma subpopulations survive at this high absorbed dose, which is in agreement with our findings of a fraction of very radio-resistant cells (Gliemroth et al, 2003).…”

Statins induce apoptosis in ovarian cancer cells through activation of JNK and enhancement of Bim expression

“…In addition to their role in lowering cholesterol, the statin family of drugs has shown a broad range of functions including suppression of cancer cell growth in vitro and in vivo [9][10][11][12][13][14][15][16] in many cell types. Sur prisingly, the eVect of statins on ovarian cancer is limited to one study documenting lovastatin-induced growth inhibi tion [35].…”

“…Recent studies have revealed that statins inhibit certain can cer cell proliferation, invasion and migration in vitro and in vivo [9][10][11][12][13][14][15][16]. To determine the direct eVect of statins on ovarian cancer cells, we treated Hey 1B cells, a human ovarian carcinoma cell line, with lovastatin, a member of the statin family, at various doses for 48 h and the lova statin-induced Hey 1B cell death could be observed by a phase contrast microscope (Fig.1a).…”

“…Statins can induce apoptosis and inhibit proliferation in a variety of cancer cell types [9][10][11][12][13]. In vivo studies have revealed that statins can inhibit tumor cell growth, invasion and metasta sis [14][15][16]. In addition, statins sensitize cancer cells to che motherapy drugs [17][18][19].…”

Radiation enhances long-term metastasis potential of residual hepatocellular carcinoma in nude mice through TMPRSS4-induced epithelial–mesenchymal transition

“…Furthermore, in some studies the invasiveness of cancer cells appears to be inhibited or unaffected by radiation. 14,15 Therefore, it is necessary to elucidate the effect of radiation on the invasion and metastatic potential of cancer cells, as well as the relevant molecular mechanisms involved. The better understanding of radiotherapy on cancer biology will certainly provide clues for intervention and improve the outcome of radiotherapy.…”