Proliferation-associated miRNAs-494, -205, -21 and -126 detected by in situ hybridization: expression and prognostic potential in breast carcinoma patients

Gurvits, Natalia; Autere, Tuomo-Artturi; Repo, Heli; Nykänen, Marjukka; Kuopio, Teijo; Kronqvist, Pauliina; Talvinen, Kati

doi:10.1007/s00432-018-2586-8

Cited by 5 publications

(2 citation statements)

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“…In the past decades, miRNAs have been intensively studied in primary breast tumor tissue leading to the identification of a number of diagnostic and prognostic miRNA signatures (23)(24)(25). Altered expression of several miRNAs, such as miR-7, miR-10a, miR-21, miR-126, miR-128, miR-205, miR-210, miR-484, miR-489, miR-494, and miR-548 have been reported in breast cancer (26)(27)(28). As minimally invasive disease biomarkers, our recent study have identified 18 diagnostic circulatory miRNA panel in breast cancer patients (22).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA Expression Profiling on Paired Primary and Lymph Node Metastatic Breast Cancer Revealed Distinct microRNA Profile Associated With LNM

et al. 2020

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Section: Introductionmentioning

confidence: 99%

MicroRNA Expression Profiling on Paired Primary and Lymph Node Metastatic Breast Cancer Revealed Distinct microRNA Profile Associated With LNM

et al. 2020

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“…MiRNAs directly bind with target genes and regulate their expression, thereby altering proliferation, 24 , 25 migration 26 and invasion of cells. 27 HIF1α is key member of the hypoxia-inducible factors family that regulates cellular response to hypoxia.…”

Section: Discussionmentioning

confidence: 99%

<p>MiR-935/HIF1α Feedback Loop Inhibits the Proliferation and Invasiveness of Glioma</p>

Huang¹,

Chen²,

Liu³

et al. 2020

OTT

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Objective The biological functions and molecular mechanisms of miR-935 have been widely investigated in various types of cancer. The aim of the present study was to explore the function of miR-935 in glioma. Methods Bioinformatic analysis and quantitative real-time fluorescent PCR (qRT-PCR) were used to determine the expression of miR-935 in glioma tissues and glioma cell lines. Chi-square test was performed to analyze the relationship between the expression of miR-935 and clinical traits. CCK-8 assay, colony formation assay, cell cycle analysis and subcutaneous tumorigenesis model in nude mice were conducted to determine the effects of miR-935 on the proliferation of glioma cells both in vitro and in vivo. Wound healing and transwell assays were used to detect the effects of miR-935 on the migration and invasion of glioma cells in vitro. The relationship between miR-935 and HIF1α was analyzed using bioinformatics, luciferase reporter assay and Western blotting. Results The expression of miR-935 was lower in glioma tissues than in the adjacent tissues, and in cell lines than in the normal human astrocytes (NHAs), and the low expression levels of miR-935 predicted a poor outcome. Exogenous overexpression of miR-935 inhibited the proliferation of glioma cells both in vitro and in vivo, and suppressed the migration and invasion of glioma cells in vitro. HIF1α was identified as the target of miR-935, whereas miR-935 overexpression decreased the expression of HIF1α and its target genes VEGF , MCL1 and GLUT1 . Strikingly, overexpression of HIF1α significantly decreased the expression of miR-935, whereas silencing HIF1α increased the expression of miR-935. Similarly, HIF1α overexpression remarkably reversed the inhibitory effects of miR-935 on the proliferation, migration and invasion of glioma cells. Conclusion Overall, present study reveals the presence of miR-935/HIF1α feedback loop in glioma, which inhibits the development of glioma. This feedback loop may be a potential target for the treatment of glioma.

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Technological advances in studying epigenetics biomarkers of prognostic potential for clinical research

Mauger

Deleuze

2019

Prognostic Epigenetics

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Proliferation-associated miRNAs-494, -205, -21 and -126 detected by in situ hybridization: expression and prognostic potential in breast carcinoma patients

Cited by 5 publications

References 56 publications

MicroRNA Expression Profiling on Paired Primary and Lymph Node Metastatic Breast Cancer Revealed Distinct microRNA Profile Associated With LNM

MicroRNA Expression Profiling on Paired Primary and Lymph Node Metastatic Breast Cancer Revealed Distinct microRNA Profile Associated With LNM

<p>MiR-935/HIF1α Feedback Loop Inhibits the Proliferation and Invasiveness of Glioma</p>

Technological advances in studying epigenetics biomarkers of prognostic potential for clinical research

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