Proliferation assays with human, rabbit, rat, and mouse lymphocytes

Liu, Forrest C.; Hoyt, David B.; Coimbra, Raúl; Junger, Wolfgang G.

doi:10.1007/bf02722976

Cited by 17 publications

(12 citation statements)

References 9 publications

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“…A negative control was represented by PBMC cultured without any mitogen/extract (C), so that the base proliferation could be estimated [22]. Therefore, nine different experimental theses for each blood sample were tested.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Ex Vivo Immune System Regulatory Properties of Boswellia serrata Extracts

Beghelli

Isani

Roncada

et al. 2017

Oxidative Medicine and Cellular Longevity

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Boswellia serrata (BS) is an important traditional medicinal plant that currently represents an interesting topic for pharmaceutical research since it possesses several pharmacological properties (e.g., anti-inflammatory, antimicrobial, and antitumour). The safety and versatility of this dietary supplement should allow for its use in numerous pathological conditions; however the quality of the extracts needs to be standardized to increase the clinical success rate resulting from its use. In the present study, different commercially available B. serrata extracts were employed to compare their AKBA content and in vitro antioxidant power. Furthermore, their ability to modulate the immune system regulatory properties was investigated. Our results showed that the AKBA content varied from 3.83 ± 0.10 to 0.03 ± 0.004%, with one sample in which it was not detectable. The highest antioxidant power and phenolic content were shown by the same extract, which also exhibited the highest AKBA concentration. Finally, the BS extracts showed the ability to influence the regulatory and effector T-cell compartments. Our results suggest that frankincense should be further investigated for its promising potentiality to modulate not only inflammation/oxidative stress but also immune dysregulation, but attention should be paid to the composition of the commercial extracts.

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Section: Methodsmentioning

confidence: 99%

Antioxidant and Ex Vivo Immune System Regulatory Properties of Boswellia serrata Extracts

Beghelli

Isani

Roncada

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized peripheral rat blood samples by density-gradient centrifugation over Ficoll-Paque (Pharmacia Biotech, Uppsala, Sweden) according to the method of Liu et al[43] (the study was approved by the Ethics Committee of the University of Torino). PBMCs were cultured in RPMI 1640 medium supplemented with 10% FCS (v/v), 2 mmol/L L -glutamine and antibiotics (100 U/mL streptomycin and 200 U/mL penicillin).…”

Section: Methodsmentioning

confidence: 99%

Solid lipid nanoparticles delivering anti-inflammatory drugs to treat inflammatory bowel disease: Effects in anin vivomodel

Dianzani¹,

Foglietta²,

Ferrara³

et al. 2017

WJG

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AIMTo improve anti-inflammatory activity while reducing drug doses, we developed a nanoformulation carrying dexamethasone and butyrate.METHODSDexamethasone cholesteryl butyrate-solid lipid nanoparticles (DxCb-SLN) were obtained with the warm microemulsion method. The anti-inflammatory activity of this novel nanoformulation has been investigated in vitro (cell adhesion to human vascular endothelial cells and pro-inflammatory cytokine release by lipopolysaccharide-induced polymorphonuclear cells) and in vivo (disease activity index and cytokine plasma concentrations in a dextran sulfate sodium-induced mouse colitis) models. Each drug was also administered separately to compare its effects with those induced by their co-administration in SLN at the same concentrations.RESULTSDxCb-SLN at the lowest concentration tested (Dx 2.5 nmol/L and Cb 0.1 μmol/L) were able to exert a more than additive effect compared to the sum of the individual effects of each drug, inducing a significant in vitro inhibition of cell adhesion and a significant decrease of pro-inflammatory cytokine (IL-1β and TNF-α) in both in vitro and in vivo models. Notably, only the DxCb nanoformulation administration was able to achieve a significant cytokine decrease compared to the cytokine plasma concentration of the untreated mice with dextran sulfate sodium-induced colitis. Specifically, DxCb-SLN induced a IL-1β plasma concentration of 61.77% ± 3.19%, whereas Dx or Cb used separately induced a concentration of 90.0% ± 2.8% and 91.40% ± 7.5%, respectively; DxCb-SLN induced a TNF-α plasma concentration of 30.8% ± 8.9%, whereas Dx or Cb used separately induced ones of 99.5% ± 4.9% and 71.1% ± 10.9%, respectively.CONCLUSIONOur results indicate that the co-administration of dexamethasone and butyrate by nanoparticles may be beneficial for inflammatory bowel disease treatment.

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“…According to the method of Liu et al [11], PBMC were isolated from heparinised peripheral rat blood samples by density-gradient centrifugation over Ficoll-Paque Premium 1.084 (Pharmacia Biotech, Sweden). Then 5×10 5 /ml viable cells were cultured in 24-well culture plates in a RPMI 1640 medium containing 10% fetal calf serum (v/v), 2 mM L-glutamine, 100 U streptomycin/ml, 200U penicillin/ml and 1 µg/ml LPS (Sigma-Aldrich, Italy) at 37 °C and 5% CO 2 for 24 h. PBMC were incubated with 1 and 10 µM CyA (diluted form commercial Sandimmun ® ) or FMOC-isoCyA (diluted from Formulation A Fluo and Formulation B Fluo and SLN Fluo) at the start of the culture period.…”

Section: Pbmc Isolation and Culturementioning

confidence: 99%

Solid Lipid Nanoparticles Loaded with Fluorescent-labelled Cyclosporine A: Anti-Inflammatory Activity In Vitro

Gallarate¹,

Serpe²,

Foglietta³

et al. 2014

PPL

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FMOC-isocyclosporine A, a fluorescent labeled cyclosporine A, was encapsulated in solid lipid nanoparticles (SLN) prepared by the coacervation technique, and its anti-inflammatory activity was evaluated. The anti-inflammatory activity of the fluorescent labelled molecule, measured as inhibition of TNF-α secretion, is similar to the native one. SLN were compared to commercial formulations, through measurement of cytokine release and drug uptake in rat peripheral blood mononuclear cells. Drug-loaded SLN inhibit TNF-α secretion in a lower extent than commercial formulations, probably due to a lower uptake by the cells, but the increase of IL-10 secretion caused by the lipid matrix itself makes this formulation interesting for its anti-inflammatory activity.

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Proliferation assays with human, rabbit, rat, and mouse lymphocytes

Cited by 17 publications

References 9 publications

Antioxidant and Ex Vivo Immune System Regulatory Properties of Boswellia serrata Extracts

Antioxidant and Ex Vivo Immune System Regulatory Properties of Boswellia serrata Extracts

Solid lipid nanoparticles delivering anti-inflammatory drugs to treat inflammatory bowel disease: Effects in anin vivomodel

Solid Lipid Nanoparticles Loaded with Fluorescent-labelled Cyclosporine A: Anti-Inflammatory Activity In Vitro

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