Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration

Mansseri-Lamrioui, Akila; Louerguioui, Ali; Bonaly, Jaqueline; Yakoub-Bougdal, Saliha; Allili, Nacer; Gana-Kebbouche, Salima

doi:10.5897/ajb11.450

Cited by 18 publications

(4 citation statements)

References 68 publications

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“…Although MS medium is still most widely used, it is often replaced by media of lower salt concentration, especially those with lower nitrogen content, including the content of ammonium nitrate which is 1650 mg•l -1 in MS medium and 1416 and 400 mg•l -1 in DKW and WPM, respectively. Most plants prefer nitrate to ammonium, and ammonium has been proved to be harmful for explants (Pierik 1997;Mansseri-Lamrioui et al 2011), because the excess of nitrate can be stored in vacuoles and high ammonium can be toxic (Glass et al 2002). On the other hand, DKW and WPM media have a higher calcium concentration compared to MS medium.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Propagation Of ‘Gisela 5’ Rootstock As Affected By Mineral Composition Of Media And Plant Growth Regulators

Fallahpour

Miri

Bouzari

2015

Journal of Horticultural Research

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The purpose of the presented study was to determine the best mineral composition of media and plant growth regulators in the micropropagation of the ‘Gisela 5’ (Prunus cerasus × P. canescens) dwarf sweet cherry rootstock. Nodal explants from young healthy shoots were excised and cultured on Murashige and Skoog medium without growth regulators. In vitro raised shoot tips were transferred to three culture media including Murashige and Skoog (MS), Driver and Kuniyuki (DKW) and Lloyd and McCown (WPM) containing benzyl adenine (BA) (0.5, 1 or 2 mg·l−1) in combination with kinetin (Kin) at 0 or 0.5 mg·l−1. WPM and DKW media were proving to be the most effective, resulting in a higher percentage of shoot multiplication and shoot number as compared to MS. BA in concentration 2 mg·l−1 resulted in the highest number of microshoots per explant (3.1). For rooting, 0, 0.5, 1 or 2 mg·l−1 indole-3-butyric acid (IBA) on MS, DKW and WPM media were tested. WPM medium containing 2 mg·l−1 IBA was most effective for rooting (93.7%) in comparison to MS (53.1%) and DKW (14.0%). Rooted plantlets were successfully hardened and established in pots.

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Section: Resultsmentioning

confidence: 99%

In Vitro Propagation Of ‘Gisela 5’ Rootstock As Affected By Mineral Composition Of Media And Plant Growth Regulators

Fallahpour

Miri

Bouzari

2015

Journal of Horticultural Research

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“…ex Godr (Marcu et al, 2014). The better effectiveness of IBA compared with NAA on rooting induction of A. occulta in this study could be attributed to several reasons including (1) more resistance to photodegradation, adherence to microshoots, and inactivation by biological action (Elmongi et al, 2018), (2) successive rooting gene activation, better uptake, transportation and stability (Liu et al, 2017), (3) slow migration and delayed degradation (Mansseri-Lamrioui et al, 2011), and (4) slower oxidization rate by the oxidase (Wolella, 2017) and thus of higher stability (Abbasi et al, 2019). It this study, the highest concentration (5 μM) of either IBA or NAA, and the simultaneous application of both auxins (IBA + NAA) irrespective their concentration combination ratios were detrimental and toxic resulting in complete rooting inhibition, which might be ascribed to ethylene synthesis induced due to high auxin concentrations (Aghaye and Yadollahi, 2012).…”

Section: In Vitro Rooting Under Different Auxin Types and Concentrationsmentioning

confidence: 73%

In vitro direct organogenesis of the medicinal single-mountain local prioritized vulnerable Greek endemic Achillea occulta under different medium variants

Sarropoulou¹,

Maloupa²,

Grigoriadou³

2023

Not Bot Horti Agrobo

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Achillea species are of high medicinal value based on their use in traditional medicine and their content in secondary metabolites with potential application in dermatology and cosmetic industry. Achillea occulta is a single-mountain local prioritized vulnerable Greek endemic encountering propagation problems through seeds and root division. In vitro propagation can be used for the rapid and mass production of clonal plants complying with phytochemical and sanitary quality criteria. In this study, several culture medium variants were tested including the effect of different indole-3-butyric acid (IBA) concentrations with 6-benzyladenine (BA), different cytokinin types, agar concentrations, and zeatin (ZT) sterilization methods on in vitro shoot proliferation, as well as the effect of different auxin types and concentrations on in vitro rooting and ex vitro survival rate of shoot-tip explants. The results showed BA was the most preferable cytokinin type as evidenced by all proliferation attributes and autoclaved ZT provided longer shoots. Therefore, the two more promising and cost-effective treatments for proliferation were 2.2 μM BA + 0.25 μM IBA or 4.4 μM BA + 0.5 μM IBA after 21 days of culture on modified Murashige-Skoog (MS) medium (x2 Fe) enriched with 20 g L-1 sucrose and 6-7 g L-1 Agar (90-93.8% shoot formation, 2.7-2.9 shoots 0.4-0.6 cm long, 2.5-2.6 proliferation rates). Explants rooted better after 21 days of culture on plain MS medium with 2 μM IBA or 1 μM α-naphthalene acetic acid (NAA) (13.3-20% rooting, 1-1.5 roots 1.8-2 cm long). NAA gave more roots, IBA longer roots, while both auxins exhibited highest rooting (13.33-20%) and ex vitro survival rates (50-66.7%).

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“…It has been shown that the majority of plants exhibit a preference for nitrate over ammonium as a source of nitrogen [66]. Previous studies have demonstrated that ammonium may have detrimental effects on explants [67,68]. This is attributed to the fact that excessive nitrate can be retained in vacuoles, whereas high levels of ammonium can be hazardous to plants.…”

Section: Evaluation Of the In Vitro Micropropagation Ability Of Black...mentioning

confidence: 99%

Usage of Machine Learning Algorithms for Establishing an Effective Protocol for the In Vitro Micropropagation Ability of Black Chokeberry (Aronia melanocarpa (Michx.) Elliott)

Demirel,

Uğur,

Popescu

et al. 2023

Horticulturae

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The primary objective of this research was to ascertain the optimal circumstances for the successful growth of black chokeberry (Aronia melanocarpa (Michx.) Elliott) using tissue culture techniques. Additionally, the study aimed to explore the potential use of machine learning algorithms in this context. The present research investigated a range of in vitro parameters such as total number of roots (TNR), longest root length (LRL), average root length (ARL), number of main roots (NMR), number of siblings (NS), shoot length (SL), shoot diameter (SD), leaf width (LW), and leaf length (LL) for Aronia explants cultivated in different media (Murashige and Skoog (MS) and woody plant medium (WPM)) with different concentrations (0, 0.5, 1, 1.5, and 2 mg L−1) of indole-3-butyric acid (IBA). The study showed that IBA hormone levels may affect WPM properties, affecting the LRL and ARL variables. Aronia explant media treated with 2 mg L−1 IBA had the greatest TNR, NMR, NS, SL, and SD values; 31.67 pieces, 2.37 pieces, 5.25 pieces, 66.60 mm, and 2.59 mm, in that order. However, Aronia explants treated with 1 mg L−1 IBA had the highest LW (9.10 mm) and LL (14.58 mm) values. Finally, Aronia explants containing 0.5 mg L−1 IBA had the greatest LRL (89.10 mm) and ARL (57.57 mm) values. In general, the results observed (TNR, LRL, ARL, NMR, NS, SL, SD, LW, and LL) indicate that Aronia explants exhibit superior growth and development in WPM (25.68 pieces, 68.10 mm, 51.64 mm, 2.17 pieces, 4.33 pieces, 57.95 mm, 2.49 mm, 8.08 mm, and 14.26 mm, respectively) as opposed to MS medium (20.27 pieces, 59.92 mm, 47.25 mm, 1.83 pieces, 3.57 pieces, 49.34 mm, 2.13 mm, 6.99 mm, and 12.21 mm, respectively). In the context of the in vitro culturing of Aronia explants utilizing MS medium and WPM, an analysis of machine learning models revealed that the XGBoost and SVM models perform better than the RF, KNN, and GP models when it comes to making predictions about those variables. In particular, the XGBoost model stood out due to the fact that it had the greatest R-squared value, and showed higher predictive ability in terms of properly forecasting values in comparison to actual outcomes. The findings of a linear regression (LR) analysis were used in order to conduct an efficacy study of the XGBoost model. The LR results especially confirmed the findings for the SD, NS, and NMR variables, whose R-squared values were more than 0.7. This demonstrates the extraordinary accuracy that XGboost has in predicting these particular variables. As a consequence of this, it is anticipated that it will be beneficial to make use of the XGboost model in the dosage optimization and estimation of in vitro parameters in micropropagation studies of the Aronia plant for further scientific investigation.

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Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration

Cited by 18 publications

References 68 publications

In Vitro Propagation Of ‘Gisela 5’ Rootstock As Affected By Mineral Composition Of Media And Plant Growth Regulators

In Vitro Propagation Of ‘Gisela 5’ Rootstock As Affected By Mineral Composition Of Media And Plant Growth Regulators

In vitro direct organogenesis of the medicinal single-mountain local prioritized vulnerable Greek endemic Achillea occulta under different medium variants

Usage of Machine Learning Algorithms for Establishing an Effective Protocol for the In Vitro Micropropagation Ability of Black Chokeberry (Aronia melanocarpa (Michx.) Elliott)

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