Proliferation and osteo/odontoblastic differentiation of stem cells from dental apical papilla in mineralization‐inducing medium containing additional KH₂PO₄

Abstract

Mineralization-inducing media supplemented with 1.8 mm additional KH2 PO4 significantly enhanced cell proliferation and improved differentiation capacity of SCAPs along osteo/odontogenic cell lineages, compared to counterparts lacking additional KH2 PO4 .

The transcription factor cyclic adenosine 3′,5′‐monophosphate response element‐binding protein enhances the odonto/osteogenic differentiation of stem cells from the apical papilla

“…, Wang et al . ). Compared with dental pulp stem cells (DPSCs), SCAPs have a higher proliferative capacity and stronger multipotent differentiation potential, which emphasizes their potential clinical therapeutic application in ‘bio‐root’ tissue engineering (Chopra et al .…”

“…Previous studies have proven that SCAPs have a strong capacity to differentiate into odonto/osteoblastlike cells in vitro under appropriate conditions and to form bone/dentine-like tissues in vivo (Huang et al 2010, Wang et al 2013. Compared with dental pulp stem cells (DPSCs), SCAPs have a higher proliferative capacity and stronger multipotent differentiation potential, which emphasizes their potential clinical therapeutic application in 'bio-root' tissue engineering (Chopra et al 2013).…”

Effects of Canonical NF-κB Signaling Pathway on the Proliferation and Odonto/Osteogenic Differentiation of Human Stem Cells from Apical Papilla

“…Diverse studies have proved that SCAPs are able to differentiate into osteo/odontoblasts in vitro under appropriate conditions and form bone/dentin-like tissues in vivo [24, 25]. Certainly, many signaling pathways may be involved in the process of cell proliferation and differentiation including NF- κ B pathway.…”

Allogenic human serum, a clinical grade serum supplement for promoting human periodontal ligament stem cell expansion

“…Additionally, different biochemical compositions of serums, such as levels of glucose, calcium, phosphorous and potassium (Cramer et al, ; Ma et al, ), might further influence the different cell behaviours in FBS and HS. It has been reported that high levels of glucose inhibit osteoblastic but promote adipogenic differentiations of MSCs (Cramer et al, ), and levels of calcium, phosphorous and potassium in culture media and local environment could regulate differentiation and bone formation capacity of MSCs (Knabe et al, ; Lai et al, ; Ma et al, ; Wang et al, ). In the current study, levels of biochemical parameters in FBS were higher than human serums, HS and Auto‐HS.…”