Proliferation and morphology of chick embryo cells cultured in the presence of horse serum and hemoglobin

Verger, C

doi:10.1007/bf02618259

Cited by 5 publications

(10 citation statements)

References 26 publications

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“…Such a possibility has been also observed with chick fibroblasts (Verger, 1979;lmbenotte and Verger, 1980) and with other cell types (Rudland et al, 1977;Young et al, 1979, Espinosa de 10s Monteros andFoucaud 1987). Some other iron-transport mechanism could be irnplicated in the absence of transferrin.…”

Section: Discussionmentioning

confidence: 76%

Proliferation of chick embryo neuroblasts grown in the presence of horse serum requires exogenous transferrin

Barakat‐Walter

Deloulme

Sensenbrenner

et al. 1991

J of Neuroscience Research

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We have previously shown that neuroblasts from cerebral hemispheres of 6-day-old chick embryos are able to proliferate when grown in the presence of fetal calf serum. We report here that in the presence of horse serum alone the proliferative rate of neuroblasts is strongly reduced. A high proliferative rate is restored upon the addition of bovine transferrin and to a lesser extent with added FeSO4 or hemin. These findings suggest that the transferrin of horse serum cannot be used by chick neuroblasts in vitro, while bovine transferrin exogenously added is active in promoting cell proliferation. We propose that the stimulatory activity of the fetal calf serum is due to bovine transferrin, since when this serum is fractionated by gel filtration, the fractions that stimulate the proliferation of neuroblasts grown in the presence of horse serum are located in the molecular weight area of transferrin, and they do contain transferrin as seen by immunoblotting with a specific anti-transferrin antibody.

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Section: Discussionmentioning

confidence: 76%

Proliferation of chick embryo neuroblasts grown in the presence of horse serum requires exogenous transferrin

Barakat‐Walter

Deloulme

Sensenbrenner

et al. 1991

J of Neuroscience Research

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“…An essential role of heme in protein synthesis has been well established iBeuzard et al, 1973;London et al, 1981;Zucker and Schulman, 1968;Waxman and Rabinovitz, 1966;Rabinovitz et al, 19691, and it has been demonstrated that iron stimulation of protein synthesis may in fact be exerted through the incorporation of iron into heme (London et al, 1981;Rabinovitz et al, 1969). Iron stimulation of cell growth may also be mediated by the chelation of the metal with protoporphyrin to form heme as the present study suggests (Verger, 1979;Imbenotte and Verger, 1980). Hemin is also known to stimulate neuronal differentiation of mouse neuroblastoma cells in culture (Ishii and Maniatis, 19781, to prevent myelin degeneration induced by lead to occur in organotypic cultures of mouse dorsal root ganglia (Whetsell et al, 19821, to promote erythroid colony formation in normal mouse bone marrow cultures (Porter et al, 19791, and to stimulate differentiation of mouse 3T3 fibroblasts to adipocytes (Chen and London, 1981).…”

Section: R)iscussimmentioning

confidence: 89%

“…Previous studies have shown that chick embryo fibroblasts do not multiply in the presence of horse serum, but that the addition of inorganic iron, hemin (Fe-protoporphyrin IX), or hemoglobin stimulates cell growth (Verger, 1979;Imbenotte and Verger, 1980). The mechanisms of action of these compounds on cell proliferation are not presently understood.…”

mentioning

confidence: 99%

Growth‐promoting effects of iron‐ and cobalt‐protoporphyrins on cultured embryonic cells

Verger

Sassa

Kappas

1983

Journal Cellular Physiology

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The effects of hemin (Fe-protoporphyrin) and Co-protoporphyrin on cellular growth have been investigated principally in cultured fibroblasts, but also in myoblasts and hepatocytes from chick embryos. In the presence of horse serum in the culture medium, which by itself did not stimulate cell growth appreciably, Co-protoporphyrin stimulated cell attachment while hemin stimulated cell proliferation of fibroblasts. When Co-protoporphyrin and hemin were added together, the most potent stimulation of cell growth, consisting of increases in cell attachment and rapid cell proliferation, was observed. These findings indicate that the two metalloporphyrins have differential and complementary effects on cellular growth in culture, with synthetic Co-protoporphyrin principally affecting cellular attachment and Fe-protoporphyrin stimulating cellular proliferation.

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“…Some cultures were seeded at 8-16 x 10jlcm' in the presence of 7% FBS or 10% HS (Institut Pasteur, Paris) without or with 40 pM FeSO, as previously described (Verger, 1979;Imbenotte and Verger, 1980).…”

Section: Culture Conditionsmentioning

confidence: 99%

“…In contrast, the addition of horse transferrin does not stimulate cell growth. I t was then concluded that the inability of HS to promote the growth of chick embryo cells is due to the incapacity of its transferrin to deliver its iron to the chick embryo cells (Verger, 1979;Imbenotte and Verger, 1980). The cultivation of chick embryo cells in the presence of HS is an adequate system for the study of the control of cell growth by iron.…”

mentioning

confidence: 99%

Decreased heme content and cessation of cell growth in cultured chick embryo fibroblasts in the presence of horse serum: Stimulation of heme synthesis and cell growth by iron

Verger

Imbenotte

1982

Journal Cellular Physiology

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A new spectrofluorometric method for heme quantitation in cultured fibroblasts is described. The method includes: 1) heme extraction by methanol/sulfuric acid, 2) partial purification of heme by a microchromatographic method, and 3) treatment of the purified heme by oxalic acid followed by fluorometric quantitation. Using this method, heme concentration was determined in chick embryo fibroblasts cultured in a medium supplemented with either 7% fetal bovine serum (FBS) or 10% horse serum (HS). In the presence of FBS, cultured cells actively divided and cells contained 34-55 pmol heme/mg protein. In contrast, cultures maintained in HS proliferated at a slower rate and contained 23-25 pmol heme/mg protein. The addition of 40 microM FeSO4 to cultures maintained in the presence of HS stimulated cell proliferation, and the cellular heme concentration increased to 37-51 pmol/mg protein. These findings suggest that the cessation of growth in the presence of HS may be due to decreased heme content in the cells and that the stimulation of cell growth by iron is mediated by its stimulation of heme synthesis.

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Proliferation and morphology of chick embryo cells cultured in the presence of horse serum and hemoglobin

Cited by 5 publications

References 26 publications

Proliferation of chick embryo neuroblasts grown in the presence of horse serum requires exogenous transferrin

Proliferation of chick embryo neuroblasts grown in the presence of horse serum requires exogenous transferrin

Growth‐promoting effects of iron‐ and cobalt‐protoporphyrins on cultured embryonic cells

Decreased heme content and cessation of cell growth in cultured chick embryo fibroblasts in the presence of horse serum: Stimulation of heme synthesis and cell growth by iron

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