Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials

Abstract

This study investigated the proliferation and differentiation potential of pluripotent mesenchymal cells on three resin-based restoratives using a typical pluripotent mesenchymal precursor cell line, C2C12. C2C12 cells were cultured for 3-21 days on cured specimens of a Bis-GMA/TEGDMA-based composite resin (APX; Clearfil AP-X), a 4-META/MMA-based resin cement (SB; Superbond C&B) or a HEMA-containing resin modified glass-ionomer (LC; Fuji Ionomer Type II LC). To examine the influences on differentiation potential, alkaline phosphatase (ALP) activity of the cells cultured on each material was determined. On APX and SB, cells adhered and proliferated well, and no significant influences on ALP activity were observed. In contrast, poor cell proliferation and significant suppression of ALP activity were observed for cells cultured on LC, similar to those cultured on a zinc oxide EBA cement used as a control material. Bis-GMA/TEGDMA-based composite resin and 4-META/MMA-based resin exhibited better biocompatibility for C2C12 cells than HEMA-containing resin modified glass-ionomer, suggesting a potential advantage of the former two resins to show smaller influences on regeneration of periapical or periodontal tissue.

Fluoride release from glass ionomer cements correlates with the necrotic death of human dental pulp stem cells

“…The Fuji I and Fuji II eluates were slightly less cytotoxic, suggesting better biocompatibility. Our results are in agreement with previous reports showing that GICs are toxic to dental pulp [16] and pluripotent mesenchymal precursor cells [17].…”

Therapeutic polymers for dental adhesives: Loading resins with bio-active components

“…In particular, several clinical studies reported the successful reconstruction of fractured roots with 4-META/MMA-based adhesive resin [77–79], which showed good bonding ability in a wet environment and high compatibility with osteoblasts or mesenchymal precursor cells [80,81]. However, none of the present adhesives available on the market promote tissue healing.…”

The elite and stochastic model for iPS cell generation: Multilineage‐differentiating stress enduring (Muse) cells are readily reprogrammable into iPS cells

“…However, the term multipotency is not adequate to describe the high differentiation ability of these cells, which are able to generate cells representative of all three germ layers. In fact, such cells are often called ‘pluripotent’ to describe their high differentiation ability (56–59). Furthermore, generation of germ line transmitting chimeras are not possible in the case of human cells.…”