Proliferation and differentiation of rat calvarial osteoblasts on type I collagen‐coated titanium alloy

Abstract

Several attempts have been made to improve osseointegration of titanium alloy as an implant material by modification of its surface. In the present study, proliferation, differentiation, and mineralization of osteoblasts on type I collagen-coated Ti6Al4V were investigated. The activity of alkaline phosphatase and the accumulation of calcium by osteoblasts grown on titanium alloy were significantly higher compared to cells grown on polystyrene. Precoating of the implant surface with type I collagen did not extensively affect proliferation, the activity of alkaline phosphatase, collagen synthesis, calcium accumulation, or the mRNA levels for collagen I alpha1, osteopontin, osteocalcin, MMP-2, and TIMP-2. Maximum collagen synthesis by osteoblasts was observed at day 4 of culture independent of the type of implant material. The specific activity of alkaline phosphatase reached its maximum at day 18 of culture. Accumulation of calcium and elevated mRNA levels for osteocalcin were found at day 22. These results indicate that collagen-coating alone is not sufficient to accelerate differentiation of rat calvarial osteoblasts on Ti6Al4V.

Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans

“…This includes an early differentiation phase characterized by expression and synthesis of ECM components, followed by ECM maturation and ending with mineralization of the ECM (74,75). Therefore, primary hBMSC served as a cellular model and were differentiated into osteoblasts by the use of an osteogenic medium.…”

Osseointegration of biochemically modified implants in an osteoporosis rodent model

“…Next, we tested whether implant coating with components of the bone extracellular matrix (ECM) would stimulate bone osseointegration in our compromised setting (Stadlinger et al, 2009a). Collagen type I is the main structural component of the bone organic ECM and has been previously demonstrated to favour the adhesion of osteoblastic cells (Becker et al, 2002). In addition, the application of glycosaminoglycans such as chondroitin sulphate (CS) influences the adhesion, proliferation and differentiation of osteoblasts (Bierbaum et al, 2006; Douglas et al, 2007).…”

Desenvolvimento do fenótipo osteoblástico em células derivadas de osso alveolar humano cultivadas sobre titânio revestido com colágeno tipo I

“…In the present study, growth analysis by MTT assay and total protein content revealed differences between Col-Ti and M-Ti only at day 3, therefore during (Becker et al 2002). Based on our results using human alveolar bonederived cells, we speculate that Col-Ti surface obtained by plasma deposition-acrylic acid grafting may accelerate cell cycle dynamics only at the first periods of the proliferative phase of the cultures.…”