Proliferation and differentiation of mononuclear phagocytes in vitro

Meer, J.W.M. van der; Gevel, Joke S. van de; Water, R. De; Ginsel, L.A.; Wouters, C. H.; Daems, Walter; Furth, R. van

doi:10.1007/978-94-009-5020-7_25

Cited by 7 publications

(1 citation statement)

References 22 publications

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“…However, bone marrow cells exhibited the greatest proliferative capacity and gave rise to more macrophage progeny than both monocytes and PEM. Previous studies showed that bone marrow stem cells rapidly differentiate into replicating adherent and nonadherent cells in response to purified CSF-1 (Stanley et al, 1983;van der Meer et al, 1985;Stewart et al, 1984). To study the role of nonadherent cells in the overall growth kinetics, both adherent and nonadherent cell numbers from bone marrow cultures were determined.…”

Section: Effect Of' Csf-1 Concentrationsmentioning

confidence: 99%

Delineation of receptor‐mediated colony‐stimulating factor (CSF‐1) utilization and cell production by precursors of mononuclear phagocytic series at various stages of differentiation

1987

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Colony-stimulating factor-1 (CSF-1) is a specific haematopoietic growth factor that stimulates the production of macrophages by both bone marrow macrophage precursors (GM-CFC) and certain more mature peripheral tissue macrophages. The relationship of CSF-1 utilization and cell production by macrophage precursors at various stages of differentiation was studied. Bone marrow GM-CFC had the highest proliferative capacity followed by blood monocytes and peritoneal exudate macrophages (PEM) as determined by their cell doubling time (DT) which was also dependent on the concentrations of exogenous CSF-1. PEM had the longest initial lag period before commencing cell proliferation. Exogenous CSF-1 was constantly utilized by the growing cells; depletion of available CSF-1 resulted in growth arrest and, subsequently, cell death. The production of macrophage progeny, per amount of CSF-1, correlated with parent macrophage maturity; for each 100 U of CSF-1 consumed, bone marrow precursor cells and blood monocytes were capable of producing 17.9 x 10(4) and 13.4 x 10(4) progeny, respectively, whereas PEM generated only 4.6 x 10(4) daughter cells. Thus, the removal and destruction of CSF-1 by more mature, less proliferative tissue macrophages may provide a possible mechanism by which CSF-1 levels are reduced and the production of early haemopoietic macrophage precursors controlled.

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Section: Effect Of' Csf-1 Concentrationsmentioning

confidence: 99%

Delineation of receptor‐mediated colony‐stimulating factor (CSF‐1) utilization and cell production by precursors of mononuclear phagocytic series at various stages of differentiation

1987

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Establishment and Characterization of Long-Term Cultured Cell Lines of Murine Resident Macrophages

Lombard¹,

Bartholeyns²,

Chokri³

et al. 1988

Journal of Leukocyte Biology

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Murine resident macrophages can proliferate in vitro when they are grown in coculture on a layer of mesothelial or endothelial type feeder cells. Resident macrophages were obtained from lung explants of C57Bl/6 lpr/lpr mice and from spleen explants or peritoneal washing of Balb/c mice; the cells were seeded without further washing. After 3-4 weeks of culture, the macrophages began to proliferate on a confluent layer of feeder cells. The macrophages then could be collected in the fluid phase and reseeded for permanent culture after generation of a new feeder layer. These cells were characterized as macrophages by the following criteria: 1) their morphology, ultrastructure, and adherence properties; 2) more than 90% of the macrophages phagocytized yeasts compared with less than 1% of the feeder cells; 3) the presence of functional Fc and mannose receptors, nonspecific cytoplasmic esterases, and membrane ectoenzymes such as nicotinamide adenine dinucleotide (NAD) glycohydrolase and nucleotide pyrophosphatase; 4) by cytofluorographic phenotype analysis with monoclonal antibodies, characterizing a normal macrophage population (MAC1+, Fcrec+, H-2K+, THY1-, LYT2-, L3T4-). 5) by functional studies proving that the expanded macrophages could function as accessory cells in the induction of lymphocyte proliferation in response to concanavalin A (Con A), that they generated reactive oxygen radicals and that they were cytotoxic for tumor cells. During coculture, growth or activating factors such as macrophage colony-stimulating factor or gamma-interferon were released in the medium. Long-term cultured macrophages had chromosomal abnormalities. Our study suggests that tissue macrophages can proliferate in vitro and hence that it is possible to establish long-term cultured cell lines of macrophages of defined and reproducible characteristics.

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Origin of Macrophages

Ginsel

1993

Blood Cell Biochemistry

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Proliferation and differentiation of mononuclear phagocytes in vitro

Cited by 7 publications

References 22 publications

Delineation of receptor‐mediated colony‐stimulating factor (CSF‐1) utilization and cell production by precursors of mononuclear phagocytic series at various stages of differentiation

Delineation of receptor‐mediated colony‐stimulating factor (CSF‐1) utilization and cell production by precursors of mononuclear phagocytic series at various stages of differentiation

Establishment and Characterization of Long-Term Cultured Cell Lines of Murine Resident Macrophages

Origin of Macrophages

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