Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent

Battista, Jiovanni A. Di; Shebaby, Wassim N.; Kizilay, Ozge; Hamade, Eva; Merhi, Raghida Abou; Mébarek, Saida; Abdallah, Dina; Badran, Bassam; Saad, Fady; Abdalla, Eddie K.; Faour, Wissam H.

doi:10.1007/s00011-014-0764-y

Cited by 22 publications

(17 citation statements)

References 41 publications

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“…The number of passages (up to passage 4) to obtain a pure population of adipose-derived stromal cells (before seeding onto the human acellular collagen matrix) with optimal angiogenic properties (VEGF secretion in hypoxia) cannot be exceeded. Several alternatives of improvement (to obtain passage 4 as quick as possible) can be postulated, such as a larger amount of harvested adipose tissue, improvement of cell expansion by the use of platelet lysate, 42,43 the use of successive subcultivation, 44 and others. Preliminary results (data not shown) demonstrated that the time of the expansion of adipose-derived stromal cells up to passage 4 can be significantly reduced by more than 50 percent.…”

Section: Discussionmentioning

confidence: 99%

Autologous Adipose Stromal Cells Seeded onto a Human Collagen Matrix for Dermal Regeneration in Chronic Wounds

Lafosse

Desmet

Aouassar

et al. 2015

Plastic and Reconstructive Surgery

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Section: Discussionmentioning

confidence: 99%

Autologous Adipose Stromal Cells Seeded onto a Human Collagen Matrix for Dermal Regeneration in Chronic Wounds

Lafosse

Desmet

Aouassar

et al. 2015

Plastic and Reconstructive Surgery

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“…Under conventional culture conditions in a dish or flask, cells are plated in 2-dimensional layer, which builts a fairly artificial environment, that has been shown to be detrimental to phenotype preservation in various cell lines. Loss of differentiation potential into osteogenic and adipogenic lineage has been described for adipose-derived stem cells 20,21 during monolayer culture. On the other hand, by 3D-cultivation in microfibers, human pluripotent stem cells have preserved their undifferentiated phenotype to a significantly higher passage than in culture on a matrigel surface 22 .…”

Section: Discussionmentioning

confidence: 99%

Spheroid formation and modulation of tenocyte-specific gene expression under simulated microgravity

Kraus

Luetzenberg

Abuagela

et al. 2017

MLTJ

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SummaryBackground: For tendon tissue engineering, tenocyte-seeded scaffolds are a promising approach. Under conventional 2D culture however, tenocytes show rapid senescene and phenotype loss. We hypothesized that phenotype loss could be counteracted by simulated microgravity conditions. Methods: Human tenocytes were exposed to microgravity for 9 days on a Random Positioning Machine (RPM). Formation of 3D-structures (spheroids) was observed under light microscopy, gene expression was measured by realtime PCR. Cells under conventional 2D-culture served as control group. Results: Simulated microgravity reached a value of as low as 0.003g. Spheroid formation was observed after 4 days, and spheroids showed stable existance to the end of the observation period. After 9 days, spheroids showed a significantly higher gene expression of collagen 1 (Col1A1) compared to adherent cells under microgravity (4.4x, p=0.04) and compared to the control group (5.6x, p=0.02). Gene expression of collagen 3 (COL3A1) was significantly increased in spheroids compared to the control group (2.3x, p=0.03). Gene expressions of the extracellular matrix genes Tenascin C und Fibronectin (TNC and FN) were increased in adherent cells under microgravity compared to the 1g-control group, not reaching statistical significance (p=0.1 and p=0.3). For the gene expression of vimentin, no significant alteration was observed both in the adherent cells and in the spheroids compared to the 1g control group. Gene expression of the tenocyte-specific transcription factor scleraxis (SCX) was significantly increased in spheroids compared to the control group (3.7x, p=0.03). Conclusion: Simulated microgravity could counteract tenocyte senescence in vitro and serve as a promising model for scaffold-free 3D cell culturing and tissue engineering. Level of evidence: V (laboratory study).

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“…Mesenchymal stem cells are one of the most important adult stem cells that have multivariate characteristics, fibroblastic morphology, high proliferation potential, and adhesion properties to the floor of plastic containers, selfrenewal, and the ability to differentiate into multiple cell lines (6)(7)(8)(9)(10). Adiposed-drived mesenchymal stem cells, in addition to the mentioned characteristics, also have other advantages, such as the lack of ability to proliferate and differentiate in long time cultures and the availability of large reserves and easy access to a large amount of fat through sampling and liposuction (11)(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Effect of hydroalcoholic extract of Cannabis (Cannabis sativa L.) on morphology and the process of human adipose-drived mesenchymal stem cell growth

et al. 2018

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Background: Today, cannabis sativa L. is the most consuming drug trafficking plant in the world after nicotine and alcohol. Aims: Given the properties of cannabis and psychoactive compounds of this opiate drug, including hashish, as well as the importance of human adipose-derived mesenchymal stem cells in the restoration and regeneration of organs and its application in tissue transplantation and tissue engineering, the current paper investigates the effect of hashish (hydroalcoholic extract of cannabis) on the morphology and growth process of human adiposed-drived mesenchymal stem cells in vitro. Methods: Human adipose-drived mesenchymal stem cells were extracted from the abdominal fat by the enzyme digestion of the patient's abdominal region. MTT test was performed to determine the best concentration of cannabis extract. Cells were treated for seven days at 100 ng / ml and 1000 ng / ml, and propagation, growth and viability of the cells were evaluated. Results:The results show that cannabis extract reduces the proliferation of human adipose-drived mesenchymal stem cells as time-dependent. Also, the effect of cannabis extract on mesenchymal cells depends on concentrations. High concentrations have a greater negative effect on the growth of the cells. The results of this study showed that the morphology of human adipose-drived mesenchymal stem cells did not change after exposure to cannabis extract. Conclusion: Although in the first days, the growth process is elevated and the cells are in the euphoric phase, apoptosis increases with time as consumption increases due to toxicity. The effects of cannabis on fat cells indicate a decrease in the volume of adipose tissue and, consequently, in the consumer's slimming.

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Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent

Abstract: Osteogenic potential of ASCs is impaired by successive passaging and may not serve as a useful clinical source of osteogenic ASCs past P2.

Cited by 22 publications

References 41 publications

Autologous Adipose Stromal Cells Seeded onto a Human Collagen Matrix for Dermal Regeneration in Chronic Wounds

Autologous Adipose Stromal Cells Seeded onto a Human Collagen Matrix for Dermal Regeneration in Chronic Wounds

Spheroid formation and modulation of tenocyte-specific gene expression under simulated microgravity

Effect of hydroalcoholic extract of Cannabis (Cannabis sativa L.) on morphology and the process of human adipose-drived mesenchymal stem cell growth

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