Proliferation and differentiation in mammalian airway epithelium

Ayers, Margaret; Jeffery, P. K.

doi:10.1183/09031936.93.01010058

Cited by 132 publications

(5 citation statements)

References 143 publications

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“…In addition, the mucus-secreting cells fulfil a role as progenitors of ciliated and other epithelial cell types [ 4,25,26). This was first suggested and hypothesized for the development of the foetal trachea of the hamster [5) and described in monkey airways [6) and in human foetal trachea also [4].…”

Section: Mucus-secreting Cellsmentioning

confidence: 95%

“…This was first suggested and hypothesized for the development of the foetal trachea of the hamster [5) and described in monkey airways [6) and in human foetal trachea also [4]. The division of surface serous and mucous cells of the rat has been shown both in the normal adult and in response to irritation by cigarette smoke [26]. The secretory cell probably, therefore, plays a major role in the repair of injured respiratory mucosa in vivo [27] and experimentally in vitro [28).…”

Section: Mucus-secreting Cellsmentioning

confidence: 99%

“…It may produce a carbohydrate (hypophase) component of surfactant [31] or an antiprotease [24,32] and is known to have ion-absorbing and secreting properties [33]. Furthermore, the Clara cell acts as the stem cell of small airways where basal and mucous cells are normally sparse: both ciliated and mucous cells may develop from the Clara cell subsequent to its division and differentiation [26]. Relatively little is known about the Clara cell during development.…”

Section: Clara Cells (Non-ciliated Bronchiolar Cells)mentioning

confidence: 99%

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Human airway secretory cells during development and in mature airway epithelium

Jeffery¹,

Gaillard²,

Moret³

1992

Eur Respir J

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The combined secretions of distinct secretory cells of the airway lining mucosa serve to keep the inspired air moist and free of potentially harmful dust particles, organisms and adsorbed gases. Apart from their role in protecting the respiratory zone of the lung, mucus-secreting cells act as pluripotential stem cells during foetal development and, in the adult, following mucosal injury. The variety of secretory cells include the mucous and serous cells of the surface and glandular epithelium, the non-ciliated bronchiolar (Clara) cell and the less frequent dense-core granulated (neuroendocrine) cell. The last-mentioned is the first type to differentiate at about 10 weeks of gestation; mucus-secreting cells are present from the 13th week of gestation, when mature ciliated cells are already present, and Clara cells begin to mature during the 19th week of human development. The alteration of secretory cell number and chemical composition of their secretions during the second trimester of foetal life is similar to that which occurs in chronic bronchitis in the adult. However, in hypersecretory disease the extent and site of the major change appear to be inappropriate to the defence of the lung.

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Section: Mucus-secreting Cellsmentioning

confidence: 95%

Section: Mucus-secreting Cellsmentioning

confidence: 99%

Section: Clara Cells (Non-ciliated Bronchiolar Cells)mentioning

confidence: 99%

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Human airway secretory cells during development and in mature airway epithelium

Jeffery¹,

Gaillard²,

Moret³

1992

Eur Respir J

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“…BSCs function as a dedicated multipotent stem cell population capable of self-renewing and differentiating to virtually every airway epithelium cell population (Fig. 2), including luminal secretory, multi-ciliated, newly identified ionocytes, and potentially SMG epithelial cells during postnatal growth, homeostasis, and epithelial repair following the loss of luminal cells [1][2][3][4][5][6]27]. Numerous studies using lineage tracing analysis and single-cell transcriptomic work also exhibited that relatively rare epithelial cell subsets such as PNECs and tuft/brush cells might descend from BSCs [28][29][30][31][32].…”

Section: Bscs Take Centre Stage In Airway Regenerationmentioning

confidence: 99%

“…The tracheal and main stem bronchi (proximal airways) of mature human and mouse lungs are lined with a pseudostratified columnar epithelium comprising basal stem cells (BSCs) and luminal airway cells. The luminal airway cells contain secretory (or club, formerly Clara) cells, multi-ciliated cells, pulmonary neuroendocrine cells (PNECs), and goblet cells [1][2][3][4][5][6][7]. Together, these cells constitute the mucociliary escalator that removes inhaled particles and pathogens stuck in the mucus via mechanical actions.…”

Section: Introductionmentioning

confidence: 99%

Roles of airway basal stem cells in lung homeostasis and regenerative medicine

Zhang

Lin

et al. 2022

Respir Res

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Airway basal stem cells (BSCs) in the proximal airways are recognized as resident stem cells capable of self-renewing and differentiating to virtually every pseudostratified epithelium cell type under steady-state and after acute injury. In homeostasis, BSCs typically maintain a quiescent state. However, when exposed to acute injuries by either physical insults, chemical damage, or pathogen infection, the remaining BSCs increase their proliferation rate apace within the first 24 h and differentiate to restore lung homeostasis. Given the progenitor property of airway BSCs, it is attractive to research their biological characteristics and how they maintain homeostatic airway structure and respond to injury. In this review, we focus on the roles of BSCs in lung homeostasis and regeneration, detail the research progress in the characteristics of airway BSCs, the cellular and molecular signaling communications involved in BSCs-related airway repair and regeneration, and further discuss the in vitro models for airway BSC propagation and their applications in lung regenerative medicine therapy.

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Localization and up-regulation of Mucin (MUC2) gene expression in human nasal biopsies of patients with cystic fibrosis

Li¹,

Wang²,

Majumdar³

et al. 1997

J. Pathol.

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Using digoxigenin–UTP‐labelled human HAM‐1 (92 bp) or SMUC41 (850 bp) cRNA probes, the expression and localization of MUC2 gene transcripts were determined by in situ hybridization in human nasal tissues obtained as biopsies from 12 patients with cystic fibrosis (CF): all had been part of a gene therapy trial in which CFTR cDNA–liposome complexes had been delivered by topical application to eight and liposome alone to four as a placebo control. For comparison, there were nasal tissues taken at surgical resection from four non‐CF subjects and a further four biopsies taken from normal healthy volunteer controls. Both SMUC41 and HAM‐1 probes provided a strong signal. MUC2 mRNA transcripts were present in serous and mucous acini of submucosal glands, ciliated and basal cells of the surface epithelium, and occasional mononuclear inflammatory cells. The percentages (mean±SEM) of serous and mucous acini showing positivity for MUC2 gene expression in the four samples surgically resected from non‐CF subjects were 25·4±5·6 and 26·7±3·3 per cent, respectively. Compared with the non‐CF subjects, the mean percentage of acini showing MUC2 gene expression in the four placebo‐treated CF subjects was significantly higher for serous (80·5±12·7 per cent; P<0·05, t‐test), but not for mucous acini (53·1±16·8 per cent; P=0·38). In CF and non‐CF groups, where present, MUC2 positivity was strongly expressed and constituted approximately 84 per cent of the cell area in serous acini, whereas it was less obvious and was confined to the perinuclear area of cells in mucous acini. A significantly greater proportion of the surface epithelium was positive for MUC2 mRNA transcripts in the CF subjects (89·0, ±1·4 per cent) than in the surgically resected tissues of the four non‐CF subjects (19·4, ±4·0 per cent) (P=0·02). In the eight CFTR‐cDNA‐treated subjects, there was an overall trend to reduction, but no statistically significant alteration of MUC2 gene expression. It is concluded that the MUC2 gene is expressed at three‐ tofour‐fold higher levels in CF nasal mucosa than in non‐CF nasal tissue and that it is expressed in a variety of cells additional to submucosal mucus‐secreting glands. © 1997 John Wiley & Sons, Ltd.

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Proliferation and differentiation in mammalian airway epithelium

Cited by 132 publications

References 143 publications

Human airway secretory cells during development and in mature airway epithelium

Human airway secretory cells during development and in mature airway epithelium

Roles of airway basal stem cells in lung homeostasis and regenerative medicine

Localization and up-regulation of Mucin (MUC2) gene expression in human nasal biopsies of patients with cystic fibrosis

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