Proliferation and apoptosis of male germ cells in captive Atlantic bluefin tuna (Thunnus thynnus L.) treated with gonadotropin-releasing hormone agonist (GnRHa)

Corriero, Aldo; Medina, Antonio; Mylonas, Constantinos C.; Bridges, C.R.; Santamaría, N.; Deflorio, M.; Losurdo, Michele; Zupa, Rosa; Gordin, H.; Gándara, Fernando de la; Ríos, Antonio Belmonte; Pousis, Chrysovalentinos; Metrio, G. De

doi:10.1016/j.anireprosci.2009.02.013

Cited by 41 publications

(34 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, the homology of FSHb and LHb to bluefin tuna was very high. Bluefin tuna is a commercially important cultured fish species, and reproductive problems such as the inhibition of FOM and a late age of sexual maturity is a serious issue (Corriero et al 2007(Corriero et al , 2009. It is difficult to analyze the physiological mechanism regulating gametogenesis in reared bluefin tuna because the adult body size is very large (30-150 kg) (Sawada et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Changes in the expression of pituitary gonadotropin subunits during reproductive cycle of multiple spawning female chub mackerel Scomber japonicus

Nyuji

Sethu

Kitano

et al. 2011

Fish Physiol Biochem

View full text Add to dashboard Cite

The endocrine regulation of reproduction in a multiple spawning fish with an asynchronous-type ovary remains largely unknown. The objectives of this study were to monitor changes in the mRNA expression of three gonadotropin (GtH) subunits (GPα, FSHβ, and LHβ) during the reproductive cycle of the female chub mackerel Scomber japonicus. Cloning and subsequent sequence analysis revealed that the cDNAs of chub mackerel GPα, FSHβ, and LHβ were 658, 535, and 599 nucleotides in length and encoded 117, 115, and 147 amino acids, respectively. We applied a quantitative real-time PCR assay to quantify the mRNA expression levels of these GtH subunits. During the seasonal reproductive cycle, FSHβ mRNA levels remained high during the vitellogenic stages, while GPα and LHβ mRNA levels peaked at the end of vitellogenesis. The expression of all three GtH subunits decreased during the post-spawning period. These results suggest that follicle-stimulating hormone (FSH) is involved in vitellogenesis, while luteinizing hormone (LH) functions during final oocyte maturation (FOM). Both GPα and FSHβ mRNA levels remained high during the FOM stages of the spawning cycle and increased further just after spawning. Thus, FSH synthesis may be strongly activated just after spawning to accelerate vitellogenesis in preparation for the next spawning. Alternatively, LHβ mRNA levels declined during hydration and then increased after ovulation. This study demonstrates that chub mackerel are a good model for investigating GtH functions in multiple spawning fish.

show abstract

Section: Discussionmentioning

confidence: 99%

Changes in the expression of pituitary gonadotropin subunits during reproductive cycle of multiple spawning female chub mackerel Scomber japonicus

Nyuji

Sethu

Kitano

et al. 2011

Fish Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…The most common dysfunctions in females are: absence of gonadal development [99]; failure of oocytes to undergo oocyte maturation (also referred to as final oocyte maturation) once vitellogenesis is completed [19, 100, 101]; or absence of spawning [102]. Production of low amount of expressible semen in males [103], as well as reduction of germ cell proliferation and increase of apoptosis [20, 104] has been reported in captive-reared male fish. Atresia of vitellogenic oocyte and failure to undergo oocyte maturation have been attributed to an insufficient pituitary luteinizing hormone (LH) release, and to the consequent steroid withdrawal, occurring in captive conditions [105, 106].…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of Reproductive Development in Wild and Captive-Reared Greater Amberjack Seriola dumerili (Risso, 1810)

et al. 2017

View full text Add to dashboard Cite

The greater amberjack Seriola dumerili is a large teleost fish with rapid growth and excellent flesh quality, whose domestication represents an ambitious challenge for aquaculture. The occurrence of reproductive dysfunctions in greater amberjack reared in captivity was investigated by comparing reproductive development of wild and captive-reared individuals. Wild and captive-reared breeders were sampled in the Mediterranean Sea during three different phases of the reproductive cycle: early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). Fish reproductive state was evaluated using the gonado-somatic index (GSI), histological analysis of the gonads and determination of sex steroid levels in the plasma, and correlated with leptin expression in the liver and gonad biochemical composition. The GSI and sex steroid levels were lower in captive-reared than in wild fish. During the ADVANCED period, when the wild greater amberjack breeders were already in spawning condition, ovaries of captive-reared breeders showed extensive atresia of late vitellogenic oocytes and spermatogenic activity ceased in the testes of half of the examined males. During the SPAWNING period, all captive-reared fish had regressed gonads, while wild breeders still displayed reproductive activity. Liver leptin expression and gonad proximate composition of wild and captive greater amberjack were similar. However, the gonads of captive-reared fish showed different total polar lipid contents, as well as specific lipid classes and fatty acid profiles with respect to wild individuals. This study underlines the need for an improvement in rearing technology for this species, which should include minimum handling during the reproductive season and the formulation of a specific diet to overcome the observed gonadal decrements of phospholipids, DHA (22:6n-3) and ARA (20:4n-6), compared to wild breeders.

show abstract

“…In captive Atlantic bluefin tuna, the administration of GnRHa through a controlled‐release delivery system (Mylonas et al., ) was correlated with an increase in plasma LH levels, as well as 11‐KT (Rosenfeld et al., ). The detection of a lower density of apoptotic germ cells in GnRHa‐treated fish was attributed to the increase in testicular 11‐KT production, which was stimulated by the GnRHa‐induced LH release from the pituitary (Corriero et al., ). It may be speculated that the differences observed in apoptotic rates between wild and captive fish may be due to differences in their corresponding plasma levels of 11‐KT, but this cannot be confirmed since no blood could be obtained from the wild fish.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of male germ cell proliferation and apoptosis in wild and captive Atlantic bluefin tuna (Thunnus thynnusL.)

Zupa

Fauvel

Mylonas

et al. 2012

Journal of Applied Ichthyology

Self Cite

View full text Add to dashboard Cite

Summary The most commonly observed reproductive dysfunction in male fishes reared in captivity is reduction in sperm volume and quality. The Atlantic bluefin tuna Thunnus thynnus (Osteichthyes: Scombridae) is one of the few large pelagic and migratory marine fishes maintained in captivity with the purpose of establishing breeding populations to support an aquaculture industry. The objectives of the present study were to compare male germ cell proliferation and apoptosis between wild and captive individuals at two different phases of the spermatogenetic cycle, and to evaluate sperm motility characteristics of captive individuals. Histological observations were performed to analyze testicular activity, and germ cell proliferation and apoptosis were evaluated through the immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and the terminal deoxynucleotidyl transferasemediated d'UTP nick end labeling (TUNEL) method, respectively. Computer‐assisted sperm analysis (CASA) was used to evaluate sperm motility. Results showed that germ cell proliferation was delayed and germ cell apoptosis increased in captive animals relative to wild individuals. Sperm motility of samples obtained from captive individuals was anomalous, both in terms of motility duration and swimming efficiency. Thus it appears that rearing in captivity impairs male reproductive function through, at least, changes in germ cell proliferation and apoptosis.

show abstract

Proliferation and apoptosis of male germ cells in captive Atlantic bluefin tuna (Thunnus thynnus L.) treated with gonadotropin-releasing hormone agonist (GnRHa)

Cited by 41 publications

References 36 publications

Changes in the expression of pituitary gonadotropin subunits during reproductive cycle of multiple spawning female chub mackerel Scomber japonicus

Changes in the expression of pituitary gonadotropin subunits during reproductive cycle of multiple spawning female chub mackerel Scomber japonicus

Comparative Study of Reproductive Development in Wild and Captive-Reared Greater Amberjack Seriola dumerili (Risso, 1810)

Comparative analysis of male germ cell proliferation and apoptosis in wild and captive Atlantic bluefin tuna (Thunnus thynnusL.)

Contact Info

Product

Resources

About