Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity

Hamada, Fumika N.; Koshiyama, Akiyo; Namekawa, Satoshi H.; Ishii, S; Iwabata, Kazuki; Sugawara, Hiroko; Nara, Takayuki; Sakaguchi, Kengo; Sawado, Tomoyuki

doi:10.1016/j.bbrc.2006.11.094

Cited by 11 publications

(17 citation statements)

References 29 publications

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“…Not only is its meiotic cell cycle long and naturally synchronous, but large populations of synchronous meiotic cells are also easy to obtain (Li et al 1999;Kües 2000;Kamada 2002;Casselton and Olesnicky 1998). These advantages have enabled us to isolate and characterize many synapsis-related proteins in C. cinereus (Nara et al 1999(Nara et al , 2001Hamada et al 2007;Ishizaki et al 2002;Namekawa et al 2003a, b, c;Yamaguchi et al 2004;Iwabata et al 2005;Koshiyama et al 2006;Sakamoto et al 2007;Sakaguchi et al 2007) and to demonstrate their roles in vivo.…”

Section: Introductionmentioning

confidence: 98%

Coprinus cinereus Mer3 is required for synaptonemal complex formation during meiosis

et al. 2008

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Mer3 is an evolutionarily conserved DNA helicase that has crucial roles in meiotic recombination and crossover formation. We have identified the MER3 homolog in Coprinus cinereus (Ccmer3) and show that it is expressed in zygotene and pachytene meiocytes. Immunostaining analysis indicated that CcMer3 was localized on chromosomes at zygotene and pachytene and CcMer3 foci were more frequent on paired than unpaired chromosomes. We generated a C. cinereus mer3 mutant (#1) and found that it showed abnormal meiosis progression and underwent apoptosis after prophase I. Basidiospore production in #1 was reduced to 0.8% of the wild-type level; the spores showed slower germination at 25 degrees C but were similar to the wild type at 37 degrees C. Electron microscopic analysis of chromosome spreads revealed that axial elements were formed in the mutant but that synapsis was defective, resulting in a reduction in spore production. Our results demonstrate that CcMer3 is required for synaptonemal complex formation after axial elements align and is thus essential for homologous synapsis.

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Section: Introductionmentioning

confidence: 98%

Coprinus cinereus Mer3 is required for synaptonemal complex formation during meiosis

et al. 2008

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“…For some years, we have investigated meiosis-related proteins and their relationships to meiotic chromosome pairing and recombination using the meiocytes of the basidiomycete, C. cinereus (Koshiyama et al 2006;Iwabata et al 2005;Hamada et al 2002Hamada et al , 2007Namekawa et al 2005;Sakaguchi and Lu 1982;Lu et al 1988;Lu and Sakaguchi 1991;Matsuda et al 1993Matsuda et al , 1996Gomi and Sakaguchi 1994;Sawado and Sakaguchi 1997;Kitamura et al 1997;Nara et al 1999Nara et al , 2000Nara et al , 2001Ishizaki et al 2002;Namekawa et al 2003a-c;Yamaguchi et al 2004;Ishibashi et al 2006a-c). Like Lilium, the meiotic process is long and naturally synchronous.…”

Section: Discussionmentioning

confidence: 96%

Two X family DNA polymerases, λ and μ, in meiotic tissues of the basidiomycete, Coprinus cinereus

et al. 2007

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The X family DNA polymerases lambda (CcPollambda) and mu (CcPolmu) were shown to be expressed during meiotic prophase in the basidiomycete, Coprinus cinereus. These two polymerases are the only members of the X family in the C. cinereus genome. The open reading frame of CcPollambda encoded a predicted product of 800 amino acid residues and that of CcPolmicro of 621 amino acid residues. Both CcPollambda and CcPolmicro required Mn(2+) ions for activity, and both were strongly inhibited by dideoxythymidine triphosphate. Unlike their mammalian counterparts, CcPollambda and CcPolmicro had no terminal deoxynucleotidyl transferase activity. Immunostaining analysis revealed that CcPollambda was present at meiotic prophase nuclei in zygotene and pachytene cells, which is the period when homologous chromosomes pair and recombine. CcPolmicro was present in a slightly wider range of cell stages, zygotene to diplotene. In analyses using D-loop recombination intermediate substrates, we found that both CcPollambda and CcPolmicro could promote primer extension of an invading strand in a D-loop structure. Moreover, both polymerases could fully extend the primer in the D-loop substrate, suggesting that D-loop extension is an activity intrinsic to CcPollambda and CcPolmicro. Based on these data, we discuss the possible roles of these polymerases in meiosis.

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“…For example, in Coprinus cinereus, PCNA specifically interacts with a meiotic specific RecA-like factor, Lim15=Dmc1 (CoLim15). It was proposed that the interaction between Lim15=Dmc1 and PCNA mediates the recombination-associated DNA synthesis during meiosis (Hamada et al, 2007). Chapman and Wolgemuth (1994) considered that the presence of PCNA in these meiotic prophase cells could reflect the function of PCNA in DNA excision repair.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning of Proliferating Cell Nuclear Antigen and Its Differential Expression Analysis in the Developing Ovary and Testis of Penaeid ShrimpMarsupenaeus japonicus

Zhang

Shen

Wang

et al. 2010

DNA and Cell Biology

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To understand the molecular mechanisms of gonadal development and maturation in penaeid shrimp Marsupenaeus japonicus, eight differentially expressed genes were obtained using a modified annealing control primer system. One of these genes is a proliferating cell nuclear antigen (PCNA). Bioinformatics analyses showed that full-length cDNA of M. japonicus PCNA (mjPCNA) consists of 75 bp of 5' untranslated region, 783 bp of coding region, and 65 bp of 3' untranslated region (excluding the polyA tail), encoding a protein of 260 amino acids with a predicted molecular mass of 28.85 kDa and an isoelectric point of 4.59. Real-time polymerase chain reaction analyses demonstrated that the gene expression level changed significantly in the developing testis and ovary. In stage 1 of ovary and testis, mjPCNA showed its lowest level during development and reached its highest expression level in stage 2 of ovary and testis. In stages 4 and 5 of ovary and the stage 3 of testis, mjPCNA held a steady expression level. Data suggest that PCNA plays an important role in the testis and ovary development, especially in the process of mitosis and meiosis.

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Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity

Cited by 11 publications

References 29 publications

Coprinus cinereus Mer3 is required for synaptonemal complex formation during meiosis

Coprinus cinereus Mer3 is required for synaptonemal complex formation during meiosis

Two X family DNA polymerases, λ and μ, in meiotic tissues of the basidiomycete, Coprinus cinereus

Molecular Cloning of Proliferating Cell Nuclear Antigen and Its Differential Expression Analysis in the Developing Ovary and Testis of Penaeid ShrimpMarsupenaeus japonicus

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