Proliferating cell nuclear antigen: a proteomics view

Abstract

Proliferating cell nuclear antigen (PCNA), a cell cycle marker protein, is well known as a DNA sliding clamp for DNA polymerase delta and as an essential component for eukaryotic chromosomal DNA replication and repair. Due to its mobility inside nuclei, PCNA is dynamically presented in a soluble or chromatin-associated form. The heterogeneity and specific modifications of PCNA may reflect its multiple functions and the presence of many binding partners in the cell. The recent proteomics approaches applied to characterizing PCNA interactions revealed multiple PCNA partners with a wide spectrum of activity and unveiled the possible existence of new PCNA functions. Since more than 100 PCNA-interacting proteins and several PCNA modifications have already been reported, a proteomics point of view seems exactly suitable to better understand the role of PCNA in cellular functions.

Deviating the level of proliferating cell nuclear antigen inTrypanosoma bruceielicits distinct mechanisms for inhibiting proliferation and cell cycle progression

“…29,30 Similar expression patterns have been reported for PCNA homologs in yeast. 31 and in the related kinetoplastid parasite, Leishmania donovani.…”

Proliferating cell nuclear antigen in the cytoplasm interacts with components of glycolysis and cancer

“…1A vs C). Annexin A2 (ANXA2), elongation factor 1 (EF1A), and glyceraldehyde-3-phosphate dehydrogenase (G3P) were shown previously to interact with PCNA [28][29][30][31][32], and the rest is reported here for the first time. The levels of three gene products involved in protein synthesis (RSSA, MDHM, and EF1A) were substantially higher in cancer than HMEC cells ( Fig.…”

Genome Protection by the 9-1-1 Complex Subunit HUS1 Requires Clamp Formation, DNA Contacts, and ATR Signaling-independent Effector Functions

“…Much knowledge about PCNA function was gained by structure/function studies that identified a PCNA hydrophobic pocket as the docking site for most PIP box motif-carrying proteins (41). Based on the structural similarity between the 9-1-1 complex and PCNA, we hypothesized that the outer surface of HUS1 would mediate physical interactions with downstream effectors.…”