Prolactin-RsaI gene polymorphism in East Anatolian Red cattle in Turkey

Sönmez, Zeynep; Özdemir, Memiş

doi:10.4314/sajas.v47i2.3

Cited by 9 publications

(10 citation statements)

References 22 publications

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“…Cattle with AA genotype have higher milk yields than cows with GA or GG genotype (24). The genotypes AA, AG, and GG have the frequency of 0.07, 0.34, and 0.59, respectively, in the livestock population (25). In addition to genetic factors, prolactin (PRL) concentrations in the circulatory system are positively correlated with photoperiodic (26), but in this study the photoperiodic of all cows was the same.…”

Section: Resultsmentioning

confidence: 55%

Reproductive efficiency and serum progesterone concentration on dairy cattle based on blood urea nitrogen (BUN) concentrations

Putri¹,

Srianto²,

Lestari³

et al. 2019

IJVS

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The objective of this study was to identify the levels of Blood Urea Nitrogen (BUN) and its relation to the reproductive efficiency and serum progesterone concentration in lactating Holstein Friesian dairy cattle. Data were obtained by stratified random sampling from population to get 18 dairy cows, which divided into three groups. The first, second, and third groups each with milk yield of less than 17, between 17-21, and over 21 liters/day, and with calving interval of less than 365, between 365-450 and more than 450 days. Blood was collected from the coccygeal vein at the time of insemination (D0), 7 day (D+7) and 22 days (D+22) after insemination. BUN and progesterone concentration were measured. Data analysis showed no significant difference (P>0.05) on the observed parameters. Data regrouping based on BUN concentration showed that the mean of services per conception (S/C) was lower and conception rate (CR) was higher (P<0.05) in dairy cattle having BUN<18 than those of with BUN>18 mg/dL. The mean of progesterone concentration at D+22 was higher (P<0.05) than those at D0 and D+7 in pregnant dairy cows and with BUN<18 mg/dL, as well as those at D+22 in non-pregnant dairy cows with BUN>18 and BUN<18 mg/dL. This study concluded that high BUN concentration affect the reproductive efficiency of dairy cows by decreasing CR and increasing progesterone serum concentration.

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Section: Resultsmentioning

confidence: 55%

Reproductive efficiency and serum progesterone concentration on dairy cattle based on blood urea nitrogen (BUN) concentrations

Putri¹,

Srianto²,

Lestari³

et al. 2019

IJVS

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“…Mahajan et al (2012)with Frieswal cattleamplified a 156 bp of the PRL gene in the exon-3 segment by PCR-RFLP method using RsaI restriction enzyme. Sonmez and Ozdemir (2017) using the PCR-RFLP method detected three fragments of the PRL gene in native EAR cattle raised in Turkey (GG genotype with 210 bp, AA genotype with 120 bp and AG genotype with 90 bp).…”

Section: Molecular Weightsmentioning

confidence: 99%

“…Patel and Chauhan (2017) found that the Chi-square values for Gir and Kankrej cattle in India were 13.46 and 12.58, indicating that the populations were not in HWE for PRL gene. Sonmez and Ozdemir (2017) applying PCR-RFLP in native East Anatolian Red cattle found that the population was in HWE for PRL gene. Bayıl and Bozkurt (2019) reported that the Holstein cattle population was in HWE for PRL gene since the Chisquare value was not significant (χ2 = 3.74).…”

Section: The Observed and Expected Number Of Alleles And Hardy-weinbe...mentioning

confidence: 99%

“…Due to the importance of this PRL candidate gene, most of the studies suggested that the PRL variants could be useful in selection programs for improving milk yield and its composition in dairy cattle (e.g. Bukhari et al, 2013;Deepika and Salar, 2014;Fontanesiet al, 2014;Sonmez and Ozdemir, 2017;Thuy et al, 2018;Dudule et al, 2020;Ozdemir, 2020, andShah et al, 2021). Recently, Ilie et al (2023) stated that the bovine PRL gene is essential for the initiation and maintenance of lactation and exerts multiple effects on mammary alveoli to promote the synthesis and secretion of major components of milk.…”

Section: Introductionmentioning

confidence: 99%

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Detecting the Molecular Associations of Prolactin Gene Genotypes With Milk Traits in Friesian and Baladi Cows Using RFLP Technique

Khalil,

Iraqi,

Mohamed

et al. 2023

Egyptian Journal of Animal Production

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Four years of experimental work were conducted applying PCR-RFLP to detect the SNP polymorphic associations of the prolactin candidate gene (PRL) with milk traits in cattle. A total of 494 lactations for 180 milking cows of Friesian in Elkarda herd (FK) and Sakha herd (FS) and Baladi cows in Elserw herd (BS) were used. The PCR products were digested using NaeI and SmaI restricted enzymes to investigate the molecular associations of the PRL gene genotypes with milk traits.The highest expected numbers of alleles (NE) in CC, CD and DD genotypes of the PRL gene were recorded in the DD genotype in both Friesian and Baladi herds, while the lowest expected numbers were recorded in CC genotype in all herds. The highest NE in GG, GT and TT genotype was in the TT genotype and the lowest NE was in the GG genotype in all herds. PRL gene was not in Hardy-Weinberg Equilibrium in all herds. The allelic frequency for the D allele was much higher than the frequency of the C allele, while the frequency of the T allele was higher than the frequency of the G allele.The observed and expected values of heterozygosity for the PRL gene were mostly moderate in the three herds studied. The values of polymorphic information content (PIC) were mostly moderate in all herds. The values of fixation index (reduction in heterozygosity due to inbreeding, FIS) were high in the FK herd (0.82), FS herd (0.62) and BS herd (0.94).The molecular associations between PRL gene genotypes and milk traits indicate that the CD genotype of the PRL gene has strong molecular associations with yields of milk, fat and protein relative to CC and DD genotypes. GG genotype was significantly associated with all milk traits relative to GT and TT genotypes.

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“…Over the years, there has been considerable interest in the genetic control of egg production and egg size in poultry, especially in chickens. The prolactin (PRL) gene consist of five exons and four introns and is one of the gene markers that affects poultry birds' egg production traits (Wang et al 2011;Sonmez and Ozdemir 2017;Yournalis et al 2019). Constant research on the PRL is therefore, critical to understanding the genetic basis of optimal egg production in chicken (Osman et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Molecular Evolution of Prolactin Gene Single Nucleotide Polymorphisms in Nigerian Chicken Ecotypes and their Association with Light Ecotype Chickens’ Egg Traits

2022

Int J Vet Sci

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The prolactin (PRL) gene influences the commencement of incubation and brooding behavior and egg production, particularly in native birds. In this study, the first experiment was performed to screen the PRL candidate gene for single nucleotide polymorphisms (SNPs) in four different morphological types of Nigerian chicken, and to evaluate their molecular evolutionary changes. To achieve this first objective, a 130-base pair (bp) fragment of PRL was sequenced in 40 different Nigerian chicken types namely, 13 Heavy Ecotype (HE) cocks and hens, 13 Light ecotype (LE) cocks and hens, 7 Frizzled Feathered (FF) cocks and 7 Naked Neck (NN) cocks. In the second experiment which aimed to associate the SNPs of the LE PRL with egg traits, 13 LE cocks and hens from the indigenous flock were screened. Three SNPs were identified in the Nigerian chicken ecotypes (A=Adenine, G=Guanine, C=Cytosine, and T=Thymine), namely G > T, T > C and A > C having allelic frequencies of 7 in NN, 10 in HE and 4 in both FF and LE chickens. Comparison of the chicken PRL (cPRL) sequence gave 98% homology with White Leghorn (Accession No. AB013783.3). The Tajima "D" test of neutral molecular evolutionary rate was 2.79, suggesting balancing selection in the cPRL. In associating the SNPs with egg traits of LE, three SNPs were identified in the LE chickens namely G > T, T > C and A > C having allelic frequencies of 4, 3 and 4, respectively. The cPRL genomic sequence consisted of 154 bp long. The T > C polymorphism showed significant (P<0.05) negative association with egg number and egg size. The findings in the second experiment showed that the homozygous TT genotypes showed better egg weight and egg number performances than the TC individuals.

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Prolactin-RsaI gene polymorphism in East Anatolian Red cattle in Turkey

Cited by 9 publications

References 22 publications

Reproductive efficiency and serum progesterone concentration on dairy cattle based on blood urea nitrogen (BUN) concentrations

Reproductive efficiency and serum progesterone concentration on dairy cattle based on blood urea nitrogen (BUN) concentrations

Detecting the Molecular Associations of Prolactin Gene Genotypes With Milk Traits in Friesian and Baladi Cows Using RFLP Technique

Molecular Evolution of Prolactin Gene Single Nucleotide Polymorphisms in Nigerian Chicken Ecotypes and their Association with Light Ecotype Chickens’ Egg Traits

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