Prolactin receptor on dissociated mammary epithelial cells at different stages of development

Sakai, Senkiti; Enami, Jumpei; Nandi, Satyabrata; Banerjee, M. R.

doi:10.1016/0303-7207(78)90087-4

Cited by 51 publications

(22 citation statements)

References 19 publications

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“…The amount of nonspecific binding was less than 10% of that of total binding, probably owing to both good iodoinsulin and undamaged mammary cells. Mammary epithelial cells dissociated by the method used are known to grow in vitro on floating collagen gels (Emerman et al, 1977) and to retain specific prolactin receptors (Sakai et al, 1978). The amount of specific binding to cells from lactating mice was higher than to cells from pregnant mice, suggesting a difference in the number of insulin receptors per cell.…”

Section: Discussionmentioning

confidence: 99%

“…The days of plug observation and parturition were counted as days 0 of pregnancy and lactation, respectively. Mammary epithelial cells dissociated with collagenase (Enami et al, 1973;Sakai et al, 1978) TCA precipitability in the medium incubated with mammary cells for 30 min was 88% of that in the fresh control medium. After 30-min incubation with mammary cells, the medium was reincubated with fresh mammary cells for another 30 min.…”

mentioning

confidence: 99%

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A method for the iodination of insulin and its binding to dissociated mouse mammary cells.

Inagaki

Kohmoto

1981

Endocrinol Japon

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A method for the iodination of insulin and its binding to dissociated mouse mammary cells.

Inagaki

Kohmoto

1981

Endocrinol Japon

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“…In general, receptor levels are high in the mammary gland from virgin animal, decrease during pregnancy, and increase again after or near delivery (121)(122)(123). Prolactin upregulates the number of its receptors, whereas progesterone antagonizes this effect of prolactin (124,125).…”

Section: Prolactinmentioning

confidence: 99%

“…Prolactin upregulates the number of its receptors, whereas progesterone antagonizes this effect of prolactin (124,125). The number of prolactin receptors on mammary cells from rabbit (122) and mouse (121) in different developmental stages varies in inverse relationship to progesterone levels in serum. Glucocorticoid increases prolactin receptors in mammary cells in culture (118,126).…”

Section: Prolactinmentioning

confidence: 99%

Growth Control and Differentiation in Mammary Epithelial Cells

Borellini¹,

Oka²

1989

Environmental Health Perspectives

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Growth and differentiation of the mammary gland are controlled by various hormones and other environmental factors The role of hormones and growth factors in mammary development is discussed with regard to animal species, physiological stages, and the various experimental systems in vitro. In the female embryo, mammary morphogenesis is induced by the mesenchyme and is hormone independent, whereas androgens cause the partial necrosis of mammary epithelium in the male. Ductal growth during adolescence requires estrogen and prolactin or growth hormone. During pregnancy, progesterone participates in the development of the lobuloaveolar structure of the gland. After parturition, changes in the hormonal environment lead to production and secretion of milk. Proliferation and differentiation of mammary epithelium can be induced in culture systems. Insulin and epidermal growth factor (EGF) stimulate mammary cell proliferation in vitro. EGF is required for the optimal growth of the mammary gland during pregnancy. EGF also appears to play an important role in mammary tumorigenesis in certain mouse strains. Production of milk proteins can be induced in vitro by the synergistic interactions of prolactin, insulin, and glucocorticoids and is inhibited by EGF and progesterone. Complete or partial sequencing of several milk protein genes and comparative analysis have led to identification of a sequence of high homology and conservation in the 5 'flanking region that is likely to be involved in the regulation of milk protein gene expression.

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“…The binding of PRL to PRL-R is minimal throughout pregnancy but begins to increase before or after parturition [7][8][9]. At present, the time-dependent change in the number of PRL-Rs especially before parturition remains unknown, since it is difficult to predict precisely the onsettime of parturition in viva.…”

Section: Introductionmentioning

confidence: 99%

Acute Expression of the PRL Receptor Gene after Ovariectomy in Midpregnant Mouse Mammary Gland.

et al. 1996

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Abstract.In order to examine the time-dependent expression of the PRL receptor (PRL-R) gene at lactogenesis, the level of PRL-R mRNA was determined following ovariectomy in pregnant mouse mammary gland. Following reverse transcription, the quantity of mRNA was measured by the competitive polymerase chain reaction. The casein (a 22000 molecular weight component) mRNA level was measured as a marker for milk synthesis. Following ovariectomy, the onset of abortion occurred mostly at 22-23 h and the level of casein mRNA began to increase at 12 h. The long and short forms of PRL-R mRNAs were detected in a molar ratio of 1:0.2 on day 12 of pregnancy. Eight h after ovariectomy, the long form of PRL-R mRNA began to increase, showing a bell-shaped profile with the highest peak at 16 h. The short form of PRL-R mRNA was at low levels and remained constant. The levels of the long form of PRL-R mRNA decreased similarly in the presence and absence of foster pups from 24 to 48 h. Conversely, casein mRNA were maintained at high levels by supplying foster pups. The level of the long form of PRL-R mRNA reached a maximum prior to abortion. The present experiments demonstrated that the acute expression of the PRL-R gene occurred in the mammary gland at lactogenesis.

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Prolactin receptor on dissociated mammary epithelial cells at different stages of development

Cited by 51 publications

References 19 publications

A method for the iodination of insulin and its binding to dissociated mouse mammary cells.

A method for the iodination of insulin and its binding to dissociated mouse mammary cells.

Growth Control and Differentiation in Mammary Epithelial Cells

Acute Expression of the PRL Receptor Gene after Ovariectomy in Midpregnant Mouse Mammary Gland.

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