Prolactin increases cell migration of MCF-7 cells without inducing an epithelium-mesenchyme transition

Lara-Reyes, Jesús A.; Castillo-García, Zuleima H.; Aranda-Abreu, Gonzálo Emiliano; Herrera-Covarrubias, Deissy; Sampieri-Ramírez, Clara L.; Aquino‐Gálvez, Arnoldo; Suárez-Medellín, Jorge M.; Hernández-Aguilar, Ma. Elena; Rojas-Durán, Fausto

doi:10.24875/j.gamo.22000006

Cited by 1 publication

(2 citation statements)

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“…For this purpose, it is necessary to analyze the behavior of the biosensor with the growth conditions needed for cell culture. RPMI-1640 medium has been found suitable for a variety of mammalian cells, including MCF-7 [ 49 , 50 , 51 , 52 ], RAW 264.7 [ 53 , 54 , 55 ], and EAhy 926 [ 56 , 57 , 58 ]. The medium contains the reducing agent glutathione and high concentrations of vitamins such as B12, inositol, choline, and p -aminobenzoic acid (PABA), including certain vitamins not found in the most common culture mediums, Eagle’s Minimal Essential Medium (EMEM) or DMEM.…”

Section: Resultsmentioning

confidence: 99%

“…The medium contains the reducing agent glutathione and high concentrations of vitamins such as B12, inositol, choline, and p -aminobenzoic acid (PABA), including certain vitamins not found in the most common culture mediums, Eagle’s Minimal Essential Medium (EMEM) or DMEM. Since RPMI-1640 contains no proteins, lipids, or growth factors, it is commonly supplemented with FBS [ 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ] which has the growth, proliferation, and adhesion factors needed for most human and animal cells. It must be kept in mind, however, that its complete composition is still unknown [ 59 ].…”

Section: Resultsmentioning

confidence: 99%

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Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems

Navarro-Nateras,

Diaz-Gonzalez,

Aguas-Chantes

et al. 2023

Biosensors

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The inclusion of online, in situ biosensors in microfluidic cell cultures is important to monitor and characterize a physiologically mimicking environment. This work presents the performance of second-generation electrochemical enzymatic biosensors to detect glucose in cell culture media. Glutaraldehyde and ethylene glycol diglycidyl ether (EGDGE) were tested as cross-linkers to immobilize glucose oxidase and an osmium-modified redox polymer on the surface of carbon electrodes. Tests employing screen printed electrodes showed adequate performance in a Roswell Park Memorial Institute (RPMI-1640) media spiked with fetal bovine serum (FBS). Comparable first-generation sensors were shown to be heavily affected by complex biological media. This difference is explained in terms of the respective charge transfer mechanisms. Under the tested conditions, electron hopping between Os redox centers was less vulnerable than H2O2 diffusion to biofouling by the substances present in the cell culture matrix. By employing pencil leads as electrodes, the incorporation of these electrodes in a polydimethylsiloxane (PDMS) microfluidic channel was achieved simply and at a low cost. Under flow conditions, electrodes fabricated using EGDGE presented the best performance with a limit of detection of 0.5 mM, a linear range up to 10 mM, and a sensitivity of 4.69 μA mM−1 cm−2.

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