Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp

Figueroa, Jaime; Molina, Alfredo; Álvarez, Marco; Villanueva, Julieta; Reyes, Ariel E.; León, Gloria; Krauskopf, Manuel

doi:10.1016/0305-0491(94)90109-0

Cited by 24 publications

(30 citation statements)

References 21 publications

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“…6). Similar to the increased expression of various pituitary factors (Figueroa et al 2005;Figueroa et al 1994) , the signals observed with C-RFa antibodies were much stronger in pituitaries from summer carp, especially in the proximal pars distalis and pars intermedia (Fig. 6,C and D).…”

Section: C-rfa Expression During Acclimatizationsupporting

confidence: 56%

“…This localization of carp C-RFa could be related to the expression of the PrRP receptor in tilapia, which was also detected in (RPD) and neurohypophysis in tilapia pituitary. In addition, it is probable that not only PRL production increases in summer carp, as described for this teleost (Figueroa et al 1994), but also the production of its hypothalamic releasing peptide, as described by real time RT-PCR in this study (Fig. 7).…”

Section: Discussionmentioning

confidence: 52%

“…Prolactin (PRL) has over 300 separate biological activities in mammals, while in fi sh it plays important roles in freshwater osmoregulation (Manzon 2002), immunomodulation , reproduction (Weber and Grau 1999) and seasonal acclimatization (Figueroa et al 1994). PRL secretion is inhibited by somatostatin in the tilapia pituitary (Grau et al 1982;Helms et al 1991) and stimulated by a gonadotropin-releasing hormone (GnRH) (Weber et al 1997) and TRH (Barry and Grau 1986).…”

Section: Introductionmentioning

confidence: 99%

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Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp

et al. 2012

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Here we show the cloning and characterization of a novel homolog of prepro C-RFa cDNA from Cyprinus carpio. The deduced preprohormone precursor of 115 amino acids leads to a mature bioactive peptide of 20 amino acids with identical sequence to other teleost C-RFa. Modeling of the mature C-RFa peptide highlighted signifi cant similarity to homologous human PrRP20, specifi cally the conserved amphipathic system defi ned by the C-terminal alpha-helix. Clearly, the synthetic C-RFa peptide stimulated prolactin release from primary cultured fi sh pituitary cells. For the fi rst time, signifi cant variation was shown in C-RFa mRNA and protein levels in the hypothalamus and pituitary between summer-and winter-acclimatized carp. Furthermore, C-RFa protein distribution in carp central nervous tissue was visualized by immunodetection in fi bers and cells in hypothalamus, olfactory tract, cerebellum and pituitary stalk. In conclusion, we demonstrated the structure conservation of C-RFa in teleosts and mammals and immunopositive cells and fi bers for C-RFa in brain areas. Finally, the increase of C-RFa expression suggests the participation of this hypothalamic factor in the mechanism of modulation in PRL expression in carp.

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Section: C-rfa Expression During Acclimatizationsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

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Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp

et al. 2012

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“…The habitat of the carp (Cyprinus carpio) undergoes seasonal changes that compel the fish to develop adaptive strategies for sensing the cyclical fluctuation of the physical parameters (e.g. temperature, photoperiod) and to transduce them into molecular signals through mechanisms that involve the neuroendocrine system (Figueroa et al, 1994;Kausel et al, 1998;López et al, 2001). One of the most outstanding cellular features of this adaptive process is the reorganization of the nucleolus architecture.…”

Section: Introductionmentioning

confidence: 99%

Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio

Vera¹,

Molina²,

Pinto

et al. 2003

Biol. Res.

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The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression of the tandem organized ribosomal genes are located in the non-transcribed intergenic spacer (IGS), we analyzed the primary structure of the carp rDNA gene IGS. The gene organization is similar to that described from other vertebrate species, including numerous repetitive sequences, the transcription start site, and some potential cis-elements such as ribosomal enhancers, proximal terminator and transcriptional terminators. Ribosomal DNA is a remarkable case of gene duplication and has been used as a model to test the concerted evolution theory. We performed sequence comparison analyses of 18S rRNA coding sequences from carp with different species, data with which an unrooted phylogram was constructed.

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“…We addressed the question as to whether, in the carp, the gradual physical external milieu changes have a functional repercussion in the hypophysis and that this gland therefore plays a role in the adaptive response of the eurythermal teleost [Figueroa et al, 1994[Figueroa et al, , 1997Kausel et al, 1999;San Martin et al, 2004]. Accordingly, we investigated environmentally induced pituitary gland gene expression and found, in the warmseason-acclimatized carp, a high level of PRL mRNAs when compared with the pituitary gland from winter-adapted fish [Figueroa et al, 1994].…”

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confidence: 99%

Gene structure and seasonal expression of carp fish prolactin short receptor isoforms

Martin¹,

Hurtado²,

Quezada³

et al. 2006

J of Cellular Biochemistry

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The complex adaptive mechanisms that eurythermal fish have evolved in response to the seasonal changes of the environment include the transduction of the physical parameter variations into neuroendocrine signals. Studies in carp (Cyprinus carpio) have indicated that prolactin (PRL) and growth hormone (GH) expression is associated with acclimatization, suggesting that the pituitary gland is a relevant physiological node in this adaptive process. Also, the distinctive pattern of expression that carp prolactin receptor (PRLr) protein depicts upon seasonal acclimatization supports the hypothesis that PRL and its receptor clearly are involved in the new homeostatic stage that the eurythermal fish needs to survive during the cyclical changes of its habitat. Here, we characterize the first prolactin receptor gene in a teleost and show that its expression is not associated with alternative promoters, unlike in humans and rodents. Using the regulatory region to direct the transcription of green fluorescent protein (GFP) in zebrafish embryos, we mapped the appearance of this hormone receptor during fish development. This is the first report identifying a fish prolactin receptor gene expressing transcript isoforms encoding for short forms of the protein (45 kDa). These have been found in osmoregulatory tissues of the carp and are regulated in connection with the seasonal acclimatization of the fish.

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Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp

Cited by 24 publications

References 21 publications

Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp

Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp

Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio

Gene structure and seasonal expression of carp fish prolactin short receptor isoforms

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