Prokaryotic diversity in continuous cropping and rotational cropping soybean soil

Abstract

In spite of the techniques based on the amplification of 16S rRNA genes (16S rDNA) to compare bacterial communities that are now widely in use in microbial ecology, little is known about the composition of the soybean continuous cropping (CC) and rotational cropping (RC) soil microbial community. To address this, we compared the levels of bacterial community diversity in RC and 5-year CC rhizosphere soil samples. We selected 407 clones in RC and 490 clones in CC for restriction fragment length polymorphism analysis. A total of 123 phylotypes were identified among the 16S rDNA clones, while 78 unique and 21 common phylotypes were identified among the CC soil isolates. Analysis of sequences from a subset of the phylotypes showed that at least 11 bacterial divisions were represented in the clone libraries. The phylotype richness, frequency distribution (evenness), and composition of the two clone libraries were investigated using a variety of diversity indices. Although the analysis of diversity indices and LIBSHUFF comparisons revealed that the compared libraries were not significantly different (P=0.05) between the RC vs. CC soils, some differences could be observed in terms of specific phyla and groups. We concluded that the group variance was not determined immediately by the cropping system's induction, but was a long-term and slow process.

Intensification of Field Pea Production: Impact on Soil Microbiology

“…In soils sampled in the same plots in 2005, soil microbial diversity measured by the phospholipid fatty acid method was also greater in the W‐P rotation than in the P‐P monoculture (Nayyar et al, 2009). Similar results have been reported, using different methods of measuring microbial diversity in soybean in China (Tang et al, 2009, Li et al, 2010) and Argentina (Vargas Gil et al, 2011), cotton in the southern United States (Acosta‐Martinez et al, 2010), and sugarcane in Australia (Pankhurst et al, 2005).…”

p-Coumaric Acid Influenced Cucumber Rhizosphere Soil Microbial Communities and the Growth of Fusarium oxysporum f.sp. cucumerinum Owen

“…A decline in the bacteria/fungi ratio was observed in ‘sick’ soils from continuous mono-cropping systems [26]. p -Coumaric acid increased the relative abundances of Firmicutes and Proteobacteria and decreased the relative abundance of Cyanobacteria , which was also found in soils under mono-cropped soybean [60]. The increase in the population of soil-borne pathogens (e.g.…”

Continuous cropping of soybean alters the bulk and rhizospheric soil fungal communities in a Mollisol of Northeast PR China

“…Using culture‐dependent methods, several studies have demonstrated that CC of soybean changes the soil microbial composition compared with rotation cropping of soybean (Chen, Yin, Wang, & Zou, ; Liu, Zhou, & Han, ; Xu et al, ; Yu, Wu, Wang, Chen, & Gu, ). Subsequently, with the development of DNA‐based molecular techniques, a few studies have shown that the rhizospheric microbial communities were different between continuous and rotation cropping of soybean (Cui, Wang, Han, & Cai, ; Li et al, ; Tang et al, ). Similar to previously reported results for bacterial communities (Liu et al, ), a clear and significant difference in soil fungal community composition was detected in this study between the two soybean cropping systems in both bulk and rhizospheric soils (Figure a; Table S6).…”