Prokaryote community dynamics in anaerobic co-digestion of swine manure, rice straw and industrial clay residuals

Abstract

The aim of this study was to analyze the effect of the addition of rice straw and clay residuals on the prokaryote methane-producing community structure in a semi-continuously stirred tank reactor fed with swine manure. Molecular techniques, including terminal restriction fragment length polymorphism and a comparative nucleotide sequence analyses of the prokaryotic 16S rRNA genes, were performed. The results showed a positive effect of clay addition on methane yield during the co-digestion of swine manure and rice straw. At the digestion of swine manure, the bacterial phylum Firmicutes and the archaeal family Methanosarcinaceae, particularly Methanosarcina species, were predominant. During the co-digestion of swine manure and rice straw the microbial community changed, and with the addition of clay residual, the phylum Bacteroidetes predominated. The new nutritional conditions resulted in a shift in the archaeal family Methanosarcinaceae community as acetoclastic Methanosaeta species became dominant.

Microbial community composition and diversity in rice straw digestion bioreactors with and without dairy manure

“…This also was observed by Callaghan et al ( 2002 ) and Dechrugsa et al ( 2013 ) who found increasing levels of manure had a detrimental effect on biogas yields. This contradicts other RS co-digestion studies, such as Estevez et al ( 2012 ), Wang et al ( 2014 ), Sahito and Mahar ( 2014 ), Li et al ( 2014a ), Xavier et al ( 2015 ) and Jiménez et al ( 2016 ), who all found manure additions positively influenced biogas yields. However, none of these positive studies compared specific CH 4 yields from RS side-by-side with and without manure addition; i.e.…”

Characterization of microbial community dynamics during the anaerobic co-digestion of thermally pre-treated slaughterhouse wastes with glycerin addition

“…Culture independent molecular techniques have increasingly been applied to the analysis of microbial communities in anaerobic digesters and have become a useful tool for the understanding of reactor performance [12]. Some works have already been published on the microbial aspects related to the co-digestion of a significant variety of organic substrates mixed with manure [13,14]. There are two main acetotrophic archaea in anaerobic digesters: those belonging to the genus Methanosaeta, which are more efficient acetate metabolism at low concentrations, and those belonging to Methanosarcina, which are more efficient at high acetate concentrations, more tolerant to stress conditions, and which can also synthetize methane via the hydrogenotrophic pathway.…”

“…to Petrimonas sulfuriphila (band6,12,13,17,19 and 20: sequence homology 92%), Clostridium disporicum (band 10, 11, 14 and 15: sequence homology 98%), Pseudomonas pertucinogena (band 8: sequence homology 95%) and Ruminofilibacter xylanolyticum (band 9 and 18: sequence homology 100%). P. sulfuriphila is a mesophilic, strictly anaerobic, fermentative bacterium that was isolated previously from a biodegraded oil reservoir[31].…”

Microbial community dynamics reflect reactor stability during the anaerobic digestion of a very high strength and sulfate‐rich vinasse

“…Only few studies have focused on microbial community characteristics , . Knowledge on the characteristics of the microbial community is essential to control and/or enhance process efficiency . For instance, the study of the microbial community dynamics could help in early detection of operational difficulties (e.g.…”

“…18,19 Knowledge on the characteristics of the microbial community is essential to control and/or enhance process efficiency. [20][21][22] For instance, the study of the microbial community dynamics could help in early detection of operational difficulties (e.g. acidification, toxicity by intermediate metabolic accumulation, microbial washout, bacteria or methanogens inhibition, etc.…”