Projection range of eDNA analysis in marshes: a suggestion from the Siberian salamander (<i>Salamandrella keyserlingii</i>) inhabiting the Kushiro marsh, Japan

Takeshita, Daiki; Terui, Shigeharu; Ikeda, Kazuko; Mitsuzuka, Takashi; Osathanunkul, Maslin; Minamoto, Toshifumi

doi:10.7717/peerj.9764

Cited by 9 publications

(7 citation statements)

References 41 publications

(49 reference statements)

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“…Our study showed spikes in mu‐ and nu‐eDNA concentrations after artificial fertilization (0 hpf) and after most of the embryos finished hatching (96 hpf). This was consistent with Takeshita et al (2020), who found concentrated eDNA emissions just after spawning and hatching, and limited emissions during the incubation phase in the egg sacs of the Siberian salamander ( Salamandrella keyserlingii ) in tank experiments. This suggests that the spikes in eDNA concentration during a breeding season in aquatic species may be used to monitor and understand the spawning and hatching events.…”

Section: Discussionsupporting

confidence: 92%

“…This suggests that the spikes in eDNA concentration during a breeding season in aquatic species may be used to monitor and understand the spawning and hatching events. Takeshita et al (2020) determined that the eDNA results from the field could reflect the number of egg sacs in the sampling sites within breeding habitats. Our study also revealed a positive relationship between the number of hatched individuals and the eDNA concentrations of Cytb and ITS1, suggesting that the eDNA copy numbers during embryo development may potentially be used to estimate the relative number of hatched larvae.…”

Section: Discussionmentioning

confidence: 99%

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Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus

Shu

Peng

2022

Environmental DNA

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Monitoring the timing, location, magnitude of spawning events, and the number of hatched larvae is important for the efficient conservation and management of fish diversity, including rare and threatened species (Fuiman & Werner, 2009;Schiemer et al., 2002). Traditional monitoring methods are time-consuming, labor-intensive, and involve detection biases that may lead to morphological misidentification and miscounts of individuals and/or eggs (Bonar et al., 2009).Reproduction surveys utilizing drift nets, seines, or bottom trawls to capture individuals and/or eggs may disturb habitats and kill fish and their eggs in nests. Alternatively, environmental DNA (eDNA) coupled with quantitative PCR analysis may be used to discern the presence/absence of target species and their relative abundance from water, without capturing the organism (Taberlet et al., 2012;Thomsen & Willerslev, 2015).Knowledge on eDNA dynamics (e.g., origin, release, and decay) during fish developmental stages is fundamental for the development of eDNA methods to monitor fish populations at different life-history stages and to interpret eDNA survey data to inform efficient conservation and management (Lacoursiere-Roussel & Deiner, 2021). Previous studies have investigated the origin and release of eDNA during spawning events in controlled laboratory environments (Bylemans et al., 2017;Tsuji & Shibata, 2020). These

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus

Shu

Peng

2022

Environmental DNA

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“…High DNA concentrations were also found in some tanks immediately after the eggs hatched (Sample ID = 4). Although it is difficult to make a simple comparison because of the difference in taxonomic groups, some studies have reported increased eDNA concentrations after hatching of eggs [ 38 ]. In contrast, when focusing on the persistence of eDNA, eDNA was detected until at least seven days after the emergence and departure of all adult mosquitoes.…”

Section: Discussionmentioning

confidence: 99%

Detection and persistence of environmental DNA (eDNA) of the different developmental stages of a vector mosquito, Culex pipiens pallens

Sakata

Sato

et al. 2022

PLoS ONE

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Preventing mosquito-borne infectious diseases requires that vector mosquitoes are monitored and controlled. Targeting immature mosquitoes (eggs, larvae, and pupae), which have less mobility than adults, is an effective management approach. However, conducting these surveys is often difficult due to the limitations of morphological classification and survey costs. The application of environmental DNA (eDNA) analysis can solve these issues because it allows easy estimation of species distribution and morphology-independent species identification. Although a few previous studies have reported mosquito eDNA detection, there is a gap in knowledge regarding the dynamics related to the persistence of immature mosquito eDNA. We used Culex pipiens pallens, a vector of West Nile fever, as a model species. First, we developed a species-specific detection assay and confirmed its specificity using in silico and in vitro tests. Next, we conducted laboratory experiments using breeding tanks. Water samples were collected at each developmental stage. In addition, water samples were collected daily until the seventh day after emergence from the pupae. We quantified eDNA using real-time PCR with the developed assay to investigate the dynamics of mosquito eDNA. The specificity of the developed assay was confirmed by in silico and in vitro tests. Mosquito eDNA was detected at all developmental stages and detected up to seven days after emergence of pupae. In particular, high concentrations of eDNA were detected immediately after hatching from eggs and after emergence from pupae. Highly frequent positive eDNA signals were continuously detected between egg hatching and pupa hatching. Mosquito eDNA was detected immediately after the eggs were introduced, and eDNA-positive detections continued until pupae emergence, suggesting that eDNA analysis is useful for monitoring mosquito larvae. In the future, monitoring immature mosquitoes using eDNA analysis will contribute to prevent mosquito-borne infectious diseases.

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“…Such eDNA signals would be useful for evaluating mosquito habitats. Although it is di cult to make a simple comparison because of the difference in taxonomic groups, some studies reported increased eDNA concentrations after eggs hatched [29]. However, eDNA is rarely detected during the egg period.…”

Section: Discussionmentioning

confidence: 99%

Detection and persistence of environmental DNA (eDNA) of a vector mosquito, Culex pipiens pallens

Sakata

Sato

et al. 2021

Preprint

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Introduction: Preventing mosquito-borne infectious diseases requires that vector mosquitoes are monitored and controlled. Targeting immature mosquitoes (eggs, larvae, and pupae), which have less mobility than adults, is an effective management approach. However, conducting these surveys is often difficult due to the limitations of morphological classification and survey costs. The application of environmental DNA (eDNA) analysis can solve these issues because it allows easy estimation of species distribution and morphology-independent species identification. Although a few previous studies have reported mosquito eDNA detection, there is a gap in knowledge regarding the dynamics of mosquito eDNA during the developmental stages of immature mosquitoes. Methods: We used Culex pipiens pallens, a vector of West Nile fever, as a model species. First, we developed a species-specific detection assay and confirmed its specificity using in silico and in vitro tests. Next, we conducted laboratory experiments using breeding tanks. Water samples were collected at each developmental stage. In addition, water samples were collected daily until the seventh day after emergence from the pupae. We quantified eDNA using real-time PCR with the developed assay to investigate the dynamics of mosquito eDNA. Results: The specificity of the developed assay was confirmed by in silico and in vitro tests. Mosquito eDNA was detected at all developmental stages and detected up to seven days after emergence of pupae. In particular, high concentrations of eDNA were detected immediately after hatching from eggs and after emergence from pupae. Highly frequent positive eDNA signals were continuously detected between egg hatching and pupa hatching. Conclusions: Mosquito eDNA was detected immediately after the eggs were introduced, and eDNA-positive detections continued until pupae emergence, suggesting that eDNA analysis is useful for monitoring mosquito larvae. The results show that eDNA analysis provides valuable information about the water sources inhabited by immature mosquitoes in mosquito control. In the future, monitoring immature mosquitoes using eDNA analysis will aid in preventing mosquito-borne infectious diseases.

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Projection range of eDNA analysis in marshes: a suggestion from the Siberian salamander (Salamandrella keyserlingii) inhabiting the Kushiro marsh, Japan

Cited by 9 publications

References 41 publications

Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus

Environmental DNA dynamics during embryonic development in the rare minnow Gobiocypris rarus

Detection and persistence of environmental DNA (eDNA) of the different developmental stages of a vector mosquito, Culex pipiens pallens

Detection and persistence of environmental DNA (eDNA) of a vector mosquito, Culex pipiens pallens

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