Proinflammatory Reaction and Cytoskeletal Alterations in Endothelial Cells after Shock Wave Exposure

Sondén, Anders; Johansson, Anne-Sofie M.; Palmblad, Jan; Kjellström, Thomas

doi:10.2310/6650.2006.05018

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Although some recent literature suggests the use of a water bath to apply shock waves to cell cultured in flasks, we applied ESWs directly to cell suspension containing tubes to avoid the nonhomogeneous effects resulting from treatment of monolayer cells in flasks. In fact, it is reported that when treating cells in flasks, there are signs of significant cell detachment and injury close to the focus region . ESW dosage is higher at the center of the culture where cells may be damaged and eventually undergo apoptosis; on the contrary, cells at the edges receiving a lower dose are positively stimulated to proliferation and differentiation …”

Section: Discussionmentioning

confidence: 99%

Extracorporeal shock waves modulate myofibroblast differentiation of adipose‐derived stem cells

Rinella

Marano

Berta³

et al. 2016

Wound Repair Regeneration

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Mesenchymal stem cells are precursors of myofibroblasts, cells deeply involved in promoting tissue repair and regeneration. However, since myofibroblast persistence is associated with the development of tissue fibrosis, the use of tools that can modulate stem cell differentiation toward myofibroblasts is central. Extracorporeal shock waves are transient short-term acoustic pulses first employed to treat urinary stones. They are a leading choice in the treatment of several orthopedic diseases and, notably, they have been reported as an effective treatment for patients with fibrotic sequels from burn scars. Based on these considerations, the aim of this study is to define the role of shock waves in modulating the differentiation of human adipose-derived stem cells toward myofibroblasts. Shock waves inhibit the development of a myofibroblast phenotype; they down-regulate the expression of the myofibroblast marker alpha smooth muscle actin and the extracellular matrix protein type I collagen. Functionally, stem cells acquire a more fibroblast-like profile characterized by a low contractility and a high migratory ability. Shock wave treatment reduces the expression of integrin alpha 11, a major collagen receptor in fibroblastic cells, involved in myofibroblast differentiation. Mechanistically, the resistance of integrin alpha 11-overexpressing cells to shock waves in terms of alpha smooth muscle actin expression and cell migration and contraction suggests also a role of this integrin in the translation of shock wave signal into stem cell responses. In conclusion, this in vitro study shows that stem cell differentiation toward myofibroblasts can be controlled by shock waves and, consequently, sustains their use as a therapeutic approach in reducing the risk of skin and tissue fibrosis.

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Section: Discussionmentioning

confidence: 99%

Extracorporeal shock waves modulate myofibroblast differentiation of adipose‐derived stem cells

Rinella

Marano

Berta³

et al. 2016

Wound Repair Regeneration

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“…Interestingly, on day 5 the measured wound area of the ESWT-treated group was still larger than the respective one of the untreated group. One possible explanation of this phenomenon is that this initially delayed wound closure could be due to an additional tissue injury caused by the highly energetic shock waves, as observed in bones [17] and endothelial cells [18, 19]. Petechial bleeding observed immediately after the shock-wave treatment lends some support to this assumption.…”

Section: Discussionmentioning

confidence: 99%

Effect of Extracorporeal Shock Wave Treatment on Deep Partial-Thickness Burn Injury in Rats: A Pilot Study

Djedovic

Kamelger

Jeschke

et al. 2014

Plastic Surgery International

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Extracorporeal shock wave therapy (ESWT) enhances tissue vascularization and neoangiogenesis. Recent animal studies showed improved soft tissue regeneration using ESWT. In most cases, deep partial-thickness burns require skin grafting; the outcome is often unsatisfactory in function and aesthetic appearance. The aim of this study was to demonstrate the effect of ESWT on skin regeneration after deep partial-thickness burns. Under general anesthesia, two standardized deep partial-thickness burns were induced on the back of 30 male Wistar rats. Immediately after the burn, ESWT was given to rats of group 1 (N = 15), but not to group 2 (N = 15). On days 5, 10, and 15, five rats of each group were analyzed. Reepithelialization rate was defined, perfusion units were measured, and histological analysis was performed. Digital photography was used for visual documentation. A wound score system was used. ESWT enhanced the percentage of wound closure in group 1 as compared to group 2 (P < 0.05). The reepithelialization rate was improved significantly on day 15 (P < 0.05). The wound score showed a significant increase in the ESWT group. ESWT improves skin regeneration of deep partial-thickness burns in rats. It may be a suitable and cost effective treatment alternative in this type of burn wounds in the future.

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“…One of the better-characterised systems is flyer-plate model, shown in Fig. 4.7 [34]. In this experimental setup a Nd-Yt-Al--garnet (Nd-YAG) laser is used to generate a pulse directed toward a fused silica window coated with a thick layer of copper.…”

Section: Laser-based Systemsmentioning

confidence: 99%

“…Cells are cultured directly in the chamber, which can be placed on an inverted microscope to enable imaging using, for example, Fig. 4.7 The flyer-plate model for application of shock waves to cell cultures with or without surface cavitation (Adapted from Sondén et al [34]). A Laser Pulse, generated with a Nd-YAG (neodymium:yttrium-aluminium-garnet) pulsed laser and monitored by an energy Meter, is reflected with a Mirror and focused with a Lens towards a Perforated Stand at the base of the sample stage.…”

Section: Mechanical Deformation Systemsmentioning

confidence: 99%

Blast Loading of Cells

Brown

2016

Blast Injury Science and Engineering

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Proinflammatory Reaction and Cytoskeletal Alterations in Endothelial Cells after Shock Wave Exposure

Abstract: SWC lesions were associated with a switch to an endothelial proinflammatory phenotype, with an impaired regeneration rate and changes in cytoskeletal functions.

Cited by 5 publications

References 31 publications

Extracorporeal shock waves modulate myofibroblast differentiation of adipose‐derived stem cells

Extracorporeal shock waves modulate myofibroblast differentiation of adipose‐derived stem cells

Effect of Extracorporeal Shock Wave Treatment on Deep Partial-Thickness Burn Injury in Rats: A Pilot Study

Blast Loading of Cells

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