Proinflammatory properties and oxidative effects of atmospheric particle components in human keratinocytes

Cervellati, Franco; Benedusi, Mascia; Manarini, Francesco; Woodby, Brittany; Russo, Mara; Valacchi, Giuseppe; Pietrogrande, M.C.

doi:10.1016/j.chemosphere.2019.124746

Cited by 42 publications

(19 citation statements)

References 56 publications

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“…We did not observe differences in transepithelial electrical resistance (TEER) between exposed and un-exposed tissues, regardless of length of exposure, likely due to the complexity of our model. However, in support of previous work [28,36,37], we observed increased levels of the oxidative stress marker 4-hydroxynonenal (4HNE) adducts in tissues exposed to PM. Since redox homeostasis is also linked to barrier function, we examined levels of tight junction proteins zonula occludens protein 1 (ZO−1) and claudin−1 and observed decreased levels of these proteins in tissues exposed to PM for 1 week and 2 weeks.…”

Section: Introductionsupporting

confidence: 90%

Particulate Matter Decreases Intestinal Barrier-Associated Proteins Levels in 3D Human Intestinal Model

Woodby

Schiavone

Pambianchi

et al. 2020

IJERPH

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(1) Background: The gastrointestinal tract (GI) tract is one of the main organs exposed to particulate matter (PM) directly through ingestion of contaminated food or indirectly through inhalation. Previous studies have investigated the effects of chronic PM exposure on intestinal epithelia in vitro using Caco−2 cells and in vivo using mice. In this study, we hypothesized that chronic PM exposure would increase epithelial permeability and decrease barrier function due to altered redox homeostasis, which alters levels and/or localization of barrier-associated proteins in human three-dimensional (3D) intestinal tissues. (2) Methods: Transepithelial electrical resistance (TEER) in tissues exposed to 50, 100, 150, 250, and 500 µg/cm2 of PM for 1 week and 2 weeks was analyzed. Levels and localization of tight junction proteins zonula occludens protein 1 (ZO−1) and claudin−1 and desmosome-associated desmocollin were analyzed using immunofluorescence. As a marker of oxidative stress, levels of 4-hydroxy-nonenal (4HNE) adducts were measured. (3) Results: No differences in TEER measurements were observed between exposed and un-exposed tissues. However, increased levels of 4HNE adducts in exposed tissues were observed. Additionally, decreased levels of ZO−1, claudin−1, and desmocollin were demonstrated. (4) Conclusion: These data suggest that chronic PM exposure results in an increase of oxidative stress; modified levels of barrier-associated proteins could possibly link to GI tract inflammatory conditions.

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Section: Introductionsupporting

confidence: 90%

Particulate Matter Decreases Intestinal Barrier-Associated Proteins Levels in 3D Human Intestinal Model

Woodby

Schiavone

Pambianchi

et al. 2020

IJERPH

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“…These results are in agreement with the previous investigation showing that PM2.5 exposure activates oxidative stress‐dependent NF‐κB pathway. [ 53,54 ]…”

Section: Discussionmentioning

confidence: 99%

Ovarian Dysfunction Induced by Chronic Whole‐Body PM2.5 Exposure

Zhou

Chen

et al. 2020

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Fine particulate matter (PM2.5) pollution arouses public health concerns over the world. Increasing epidemiologic evidence suggests that exposure to ambient airborne PM2.5 increases the risk of female infertility. However, relatively few studies have systematically explored the harmful effect of chronic PM2.5 exposure on ovarian function and the underlying mechanisms. In this study, female C57BL/6J mice are exposed to filtered air or urban airborne PM2.5 for 4 months through a whole‐body exposure system. It is found that PM2.5 exposure significantly caused the alteration of estrus cycles, reproductivity, hormone levels, and ovarian reserve. The granulosa cell apoptosis via the mitochondria dependent pathway contributes to the follicle atresia. With RNA‐sequencing technique, the differentially expressed genes induced by PM2.5 exposure are mainly enriched in ovarian steroidogenesis, reactive oxygen species and oxidative phosphorylation pathways. Furthermore, it is found that increased PM2.5 profoundly exacerbated ovarian oxidative stress and inflammation in mice through the NF‐κB/IL‐6 signaling pathway. Notably, dietary polydatin (PD) supplement has protective effect in mice against PM2.5‐induced ovarian dysfunction.These striking findings demonstrate that PM2.5 and/or air pollution is a critical factor for ovarian dysfunction through mitochondria‐dependent and NF‐κB/IL‐6‐mediated pathway, and PD may serve as a pharmaceutic candidate for air pollution‐associated ovarian dysfunction.

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“…Transition metals (e.g., copper and iron) present in PM can generate ROS through Fenton reactions [192]. In keratinocytes, redox-active components such as copper and quinones were shown to induce oxidative stress by means of inducing the nuclear translocation of a redox-sensitive protein complex, causing mitochondrial stress and finally leading to apoptosis [193]. The redox activity of PM is correlated to the size and the adsorbed species on the surface [194].…”

Section: Pm Triggers Exogenous and Endogenous Ros Formationmentioning

confidence: 99%

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Dijkhoff¹,

Drašler²,

Karakoçak³

et al. 2020

Part Fibre Toxicol

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116

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Background: Air pollution is killing close to 5 million people a year, and harming billions more. Air pollution levels remain extremely high in many parts of the world, and air pollution-associated premature deaths have been reported for urbanized areas, particularly linked to the presence of airborne nano-sized and ultrafine particles. Main text: To date, most of the research studies did focus on the adverse effects of air pollution on the human cardiovascular and respiratory systems. Although the skin is in direct contact with air pollutants, their damaging effects on the skin are still under investigation. Epidemiological data suggested a correlation between exposure to air pollutants and aggravation of symptoms of chronic immunological skin diseases. In this study, a systematic literature review was conducted to understand the current knowledge on the effects of airborne particulate matter on human skin. It aims at providing a deeper understanding of the interactions between air pollutants and skin to further assess their potential risks for human health. Conclusion: Particulate matter was shown to induce a skin barrier dysfunction and provoke the formation of reactive oxygen species through direct and indirect mechanisms, leading to oxidative stress and induced activation of the inflammatory cascade in human skin. Moreover, a positive correlation was reported between extrinsic aging and atopic eczema relative risk with increasing particulate matter exposure.

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Proinflammatory properties and oxidative effects of atmospheric particle components in human keratinocytes

Cited by 42 publications

References 56 publications

Particulate Matter Decreases Intestinal Barrier-Associated Proteins Levels in 3D Human Intestinal Model

Particulate Matter Decreases Intestinal Barrier-Associated Proteins Levels in 3D Human Intestinal Model

Ovarian Dysfunction Induced by Chronic Whole‐Body PM2.5 Exposure

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

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