Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNA

Bachtiar, Boy M.; Bachtiar, Endang Winiati

doi:10.1186/s13104-017-2740-4

Cited by 15 publications

(12 citation statements)

References 31 publications

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“…RNA isolation, purification, and reverse transcription of cDNA were performed similarly those in the previous study 19 . Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen Life Technologies, Carlsbad, CA, USA), passive reference (ROX, Invitrogen), and S. mutans gtfB primers ( Table 1 ), as well as 1 µg of cDNA, were used to quantify the cDNA, and non-transcribed RNA samples were used as control for genomic DNA contamination.…”

Section: Methodsmentioning

confidence: 99%

Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

Bachtiar

2018

F1000Res

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The aim of this study was to analyze the synergistic Background: relationship between and in Candida albicans Streptococcus mutans children with early childhood caries (ECC) experience.Dental plaque and unstimulated saliva samples were taken from Methods: 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance of and and relative to C. albicans S. mutans total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase ( ) gene in dental plaque. Student's t-test and gtfB Pearson's correlation were used to perform statistical analysis.Within the ECC group, the quantity of both microorganisms were Results: higher in the saliva than in dental plaque. The ratio of to total C. albicans bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite for (p < 0.05). The different value of S. mutans C. and in saliva was positively correlated, and negatively albicans S. mutans correlated in dental plaque. Transcription level of showed a S. mutans gtfB positive correlation with concentration in dental plaque. C. albicans has a positive correlation with cariogenic traits of Conclusion: C. albicans in ECC-related biofilm of young children. S. mutans

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Section: Methodsmentioning

confidence: 99%

Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

Bachtiar

2018

F1000Res

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“…In order to shed more light on the different behavior observed in these strains, we also determined their influence on the gene expression of IL-6, TNF-α, TGF-β1, and GAPDH as well as Nrf2 and its downstream effector heme oxygenase 1 (HO-1). The human MG-63 osteoblast-like cell line was selected not only because it represents a validated model to study the pro-inflammatory response to bacterial infection [27][28][29], but also because shows a number of features typical of an undifferentiated osteoblast phenotype including the synthesis of collagen types I and III, a low basal expression of alkaline phosphatase which is increased following 1,25-dihydroxyvitamin D (1,25(OH)2D) administration, and the production of osteocalcin in the presence of 1,25(OH)2D [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Different Modulatory Effects of Four Methicillin‐Resistant <em>Staphylococcus aureus</em> Clones on MG-63 Osteoblast-Like Cells

Musso

Caruso

Bongiorno

et al. 2020

Preprint

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Staphylococcus aureus is a Gram-positive bacterium causing a range of mild to life-threatening infections including bone infections such as osteomyelitis. S. aureus is able to invade and persist within non-professional phagocytic cells such as osteoblasts. In the present study four different S. aureus strains, 2SA-ST239-III, 5SA-ST5-II, 10SA-ST228-I, and 14SA-ST22-IVh were tested for their ability to modulate cell viability in MG-63 osteoblast-like cells following a successful invasion and persistence. Methicillin-sensitive S. aureus (MSSA) ATCC-12598-ST30 was used as control. Despite the demonstrated similar abilities of internalization and persistence of ATCC-12598-ST30, 2SA-ST239-III, and 14SA-ST22-IVh strains in MG-63 osteoblast-like cells under our experimental conditions, we demonstrated that the decrease in cell viability was due to the different behavior of the considered strains, with the number of intracellular bacteria playing a limited role. We focused our attention on different cellular biochemical functions related to inflammation, cell metabolism, and oxidative stress during osteoblast infections. We were able to show that: 1) ATCC-12598-ST30 and 2SA-ST239-III were the only two clones able to persist and maintain their number into the cellular hostile environment during the entire period of infection; 2) 2SA-ST239-III was the only clone able to significantly increase the gene expression (3 and 24 h) and protein secretion (24 h) of both interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in MG-63 osteoblast-like cells; 3) the same clone determined a significant up-regulation of transforming growth factor-β1 (TGF-β1) and the metabolic marker glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs at 24 h post infection; 3) neither the MSSA nor the four MRSA strains induced oxidative stress phenomena in MG-63 cells, although a very different expression pattern towards nuclear factor E2-related factor 2 (Nrf2) and its downstream gene heme oxygenase 1 (HO-1) activation was observed among the different clones. Our results can open a new way of considering therapies, going in the direction of an individualized therapeutic strategy that should take into account the difference existing between MSSA and MRSA as well as the distinctive features of the different clones. Not only, therefore, a different antibiotic approach but also a starting point for considering different host factors, i.e. the modulation of specific cytokines such as IL-6, TNF-α, and TGF-β1.

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“…Relative expression of the target gene normalized to GAPDH, gene expression was analyzed using the 2 -ΔΔCt method and compared to control. The gene expression of TNF, IL1B and NOS2 were evaluated by RT-qPCR as previously reported 15 Results Figure 2 shows the microscopic morphology of the SHSY-5Y cells that were not exposed to P. gingivalis, and those exposed to P. gingivalis and coated with anti-P. gingivalis antibodies. From these figures, it is known that cells not exposed to P. gingivalis grew more than cells exposed to P. gingivalis, both with and without antibodies.…”

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 97%

Expression of TNF, IL1B, and NOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis

et al. 2020

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Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti-P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B, NOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti-P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and NOS2 of neuron cells.

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Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNA

Cited by 15 publications

References 31 publications

Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

Different Modulatory Effects of Four Methicillin‐Resistant <em>Staphylococcus aureus</em> Clones on MG-63 Osteoblast-Like Cells

Expression of TNF, IL1B, and NOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis

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