Proinflammatory cytokine expression in cervicovaginal secretions of normal and HIV-infected women

Bélec, Laurent; Gherardi, Romain K.; Payan, Christopher; Prazuck, Thiérry; Malkin, Jean-Elie; Tévi-Bénissan, Carol; Pillot, J.

doi:10.1006/cyto.1995.0077

Cited by 74 publications

(51 citation statements)

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“…Raised levels of TNF-a have been demonstrated in the peripheral blood of HIV1 patients. Belec et al also showed increased levels of this proinflammatory cytokine in both blood and cervicovaginal washings of HIV1 women [61]. The results of our study, however, showed a definite fall in TNF-a levels in the lower genital tract of the HIV1 group.…”

Section: Discussioncontrasting

confidence: 73%

Immunity in the Female Lower Genital Tract and the Impact of HIV Infection

Ahmed¹,

Al-Doujaily²,

Johnson

et al. 2001

Scand J Immunol

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This study investigates the distribution of immunocompetent cells in the ectocervix, and cytokine and immunoglobulin (Ig) levels in cervicovaginal secretions to determine whether they are altered in asymptomatic human immunodeficiency virus (HIV) infection. Ectocervical biopsies from 10 HIV1 and 10 presumed HIV-ve women were studied by immunocytochemistry. Levels of Igs in cervicovaginal secretions were quantified by radial immunodiffusion (RID) and cytokine levels by ELISA. HIV1 women had significantly increased numbers of CD8 1 lymphocytes resulting in reversal of the CD4:CD8 ratio. There was a significant increase in the proportion of activated CD81 HLA-DR1 and CD4 1 HLA-DR 1 lymphocytes, but not in CD81 TIA-11 cells. The epithelium of the cervix from HIV1 subjects showed a significant increase in both numbers of macrophages (CD681) and proportions of activated macrophages (CD681 HLA-DR1) compared to normal. The stroma contained increased proportions of inductive (D11) and suppressive (D11 D71) macrophages but a decrease in effector phagocyte (D71) proportions and Langerhans' cells. Significantly lower tumour necrosis factor (TNF)-a levels were observed in cervicovaginal secretions from HIV1 subjects. IgG levels were 4 times higher and IgM levels twice higher in cervicovaginal secretions from HIV1 women, compared to results from normal subjects. These results suggest a response within the CD8 1 cells in HIV1 women, yet these cells may have a low cytolytic capacity. The raised proportions of HLA-DR1 and D11 CD4 1 macrophages could act as antigen-presenting cells (APC) for CD4 1 CD45RO1 lymphocytes, and represent a local acquired response. However, the close juxtaposition of these cells offers the potential for them to act as a local reservoir of virus and promote its proliferation. The increase of IgG over sIgA in secretions of HIV1 subjects provides evidence suggesting a dysregulation of local humoral immunity.

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Section: Discussioncontrasting

confidence: 73%

Immunity in the Female Lower Genital Tract and the Impact of HIV Infection

Ahmed¹,

Al-Doujaily²,

Johnson

et al. 2001

Scand J Immunol

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“…Interestingly, hypoxic stress, a common environmental feature of solid tumors, has been demonstrated to induce IL-6 expression in vascular cells and cardiomyocytes (YamauchiTakihara et al, 1995;Yan et al, 1995), implying that IL-6 may be an important mediator in hypoxia-induced angiogenesis during rapid tumor growth. IL-6 is a key microenvironmental influence in cervical cancer, and is produced in different types of cells in cervical cancer, including tumor cells, fibroblasts, macrophages, and monocytes (Belec et al, 1995;Iglesias et al, 1995;Tartour et al, 1994;Wei et al, 2001a). Accordingly, the data herein indicated that IL-6 may transcriptionally upregulate VEGF expression in cervical cancer cells via an autocrine and/or paracrine mechanism.…”

Section: Discussionmentioning

confidence: 59%

Interleukin-6 promotes cervical tumor growth by VEGF-dependent angiogenesis via a STAT3 pathway

et al. 2003

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“…Women were included on the basis of lack of sexually transmitted diseases, HIV-seronegativity, and absence of menstruation. Following the introduction of a speculum, a standardized 60-s lavage was performed with 10 ml of 1ϫ PBS (pH 7.2), as described previously (23). All samples were processed within 1 h of collection.…”

Section: Cervicovaginal Secretionsmentioning

confidence: 99%

Differential Modulation of Human Lactoferrin Activity against Both R5 and X4-HIV-1 Adsorption on Epithelial Cells and Dendritic Cells by Natural Antibodies

Saïdi

Eslaphazir

Carbonneil

et al. 2006

The Journal of Immunology

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Human lactoferrin (Lf) is an iron binding glycoprotein that is present in several mucosal secretions. Many biological functions have been ascribed to Lf. In the present study, we showed that Lf limited specifically adsorption of R5- and X4-HIV-1-free particles on endometrial epithelial HEC-1A cells, by inhibiting virus adsorption on heparan-sulfated proteoglycans. But, Lf did not interfere with both R5 and X4-HIV transcytosis. We showed also the efficacy of Lf in preventing R5 and X4-HIV capture by dendritic cells. Conversely, we demonstrated that Lf-reacting natural Abs (NAbs) present within i.v. Ig-enhanced HIV attachment on dendritic cells by forming HIV-Lf-NAbs. HIV particles were able to directly interact with Lf following its interaction with NAbs. We also found Lf-reacting natural Abs within cervicovaginal secretions, suggesting the existence of Lf-NAbs complexes in women genital tract in vivo. In conclusion, this study highlights Lf as a potent microbicides and reports new function for NAbs within the genital compartment that may compartment that may abolish the inhibitory activity of microbicide compounds. Thus, we proposed a model in which Lf would appear as a double-edged sword that could have beneficial or detrimental effects depending on both cellular and molecular environments. This study highlights the use of Lf derivates as microbicide candidates to limit such interferences.

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Proinflammatory cytokine expression in cervicovaginal secretions of normal and HIV-infected women

Cited by 74 publications

References 0 publications

Immunity in the Female Lower Genital Tract and the Impact of HIV Infection

Immunity in the Female Lower Genital Tract and the Impact of HIV Infection

Interleukin-6 promotes cervical tumor growth by VEGF-dependent angiogenesis via a STAT3 pathway

Differential Modulation of Human Lactoferrin Activity against Both R5 and X4-HIV-1 Adsorption on Epithelial Cells and Dendritic Cells by Natural Antibodies

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