Prohibitin as an oxidative stress biomarker in the eye

Abstract

Identification of biomarker proteins in the retina and the retinal pigment epithelium (RPE) under oxidative stress may imply new insights into signaling mechanisms of retinal degeneration at the molecular level. Proteomic data from an in vivo mice model in constant light and an in vitro oxidative stress model are compared to controls under normal conditions. Our proteomic study shows that prohibitin is involved in oxidative stress signaling in the retina and RPE. The identity of prohibitin in the retina and the RPE was studied using 2D electrophoresis, immunohistochemistry, western blot, and mass spectrometry analysis. Comparison of expression levels with apoptotic markers as well as translocation between mitochondria and the nucleus imply that the regulation of prohibitin is an early signaling event in the RPE and retina under oxidative stress. Immunohistochemical analysis of murine aged and diabetic eyes further suggests that the regulation of prohibitin in the RPE/retina is related to aging- and diabetes-induced oxidative stress. Our proteomic approach implies that prohibitin in the RPE and the retina could be a new biomarker protein of oxidative stress in aging and diabetes.

Altered Cytoskeleton as a Mitochondrial Decay Signature in the Retinal Pigment Epithelium

“…A narrow range of isoelectric focusing was performed for the analysis of vimentin modifications (Figure 5B). Previously, we presented data from in vivo model from other genetic background (C57BL/6J) under constant light (7 days), intense light (7000-10,000 lux), or circadian regulated (12 h light/12 h dark) as well as C3HeB/FeJ model [1,3,33]. Prohibitin signaling and cytoskeletal protein expressions are consistent in mice model with two different genetic background (BL6 vs C3He), however, only albino mice showed different prohibitin/cytoskeletal protein expressions (data not shown).…”

New Biomarkers in the Retina and RPE Under Oxidative Stress

“…We have focused on understanding the cell death mechanism of the retina and RPE under oxidative stress [Chung et al, 2009;Lee et al, 2010a;Lee et al, 2010b;Zhang et al, 2010;Lee et al, 2011;Arnouk et al, 2011;Sripathi et al, 2011]. Our studies demonstrated that oxidative stress may trigger induction of anti-apoptotic erythropoietin, JAK2, and BCL-xL, as well as pro-apoptotic caspases.…”

“…This approach demonstrated that prohibitin is involved in oxidative stress signaling in vitro and in vivo [Lee et al, 2010b;Arnouk et al, 2011;Srinivas et al, 2011]. Prohibitin was proposed as an anti-proliferative protein or a tumor suppressor forming a high molecular complex with prohibitin2 in mitochondria [Steglich et al, 1999].…”

Intralesional Human Recombinant Epidermal Growth Factor for the Treatment of Advanced Diabetic Foot Ulcer: From Proof of Concept to Confirmation of the Efficacy and Safety of the Procedure

“…Prohibitin is an inhibitor of cell cycle progression and may therefore contribute to the onset of wound's chronic phenotype. (Mishra et al, 2010;Lee et al, 2010;Dong et al 2010). This expression profile became progressively inverted going through deeper cells layers (Fig.…”