Progressive increase of CD4+/CD45RC− lymphocytes after allograft rat lung transplantation: A marker of acute rejection

Takehisa, Yoshifumi; Shoji, Shuichi; Uyama, Tadashi; Sho, Masayuki; Tamaki, Masanori; Hino, Hitoshi; Takehisa, Masatsugu; Liu, Mingyao; Kondo, Kazuya; Monden, Yasumasa

doi:10.1067/mtc.2002.126043

Cited by 7 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6B). Similar to a prior report (28), there are few RC high cells recoverable from untreated rat lung allograft recipients. Therefore, not all culture conditions could be performed with these cells as compared with studies in Fig.…”

Section: Compared With Other Groups) B Reduction Of Dth Responses Tsupporting

confidence: 71%

Differential Expression of Smad7 Transcripts Identifies the CD4+CD45RChigh Regulatory T Cells That Mediate Type V Collagen-Induced Tolerance to Lung Allografts

Mizobuchi

Yasufuku

Zheng

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

Regulatory T cells (Tregs) induced by oral tolerance may suppress immunity by production of TGF-β that could also enhance Treg activity. However, all cells that are phenotypically Tregs in rats (CD4+CD45RChigh-RChigh) may not have regulatory function. Because Smad7 expression in T cells is associated with inflammation and autoimmunity, then lack of Smad7 may identify those cells that function as Tregs. We reported that feeding type V collagen (col(V)) to WKY rats (RT1l) induces oral tolerance to lung allografts (F344-RT1lvl) by T cells that produce TGF-β. The purpose of the current study was to identify the Tregs that mediate col(V)-induced tolerance, and determine Smad7 expression in these cells. RChigh cells from tolerant rats were unresponsive to allogeneic stimulation and abrogated rejection after adoptive transfer. In contrast, CD4+CD45RClow (RClow) cells from tolerant rats and RChigh or RClow cells from normal rats or untreated allograft recipients proliferated vigorously in response to donor Ags, and did not suppress rejection after adoptive transfer. TGF-β enhanced proliferation in response to col(V) presented to tolerant RChigh, but not other cells. In contrast to other cells, only RChigh cells from tolerant rats did not express Smad7. Collectively, these data show that the Tregs that mediate col(V)-induced tolerance to lung allografts do not express SMAD7 and, therefore, are permissive to TGF-β-mediated signaling.

show abstract

Section: Compared With Other Groups) B Reduction Of Dth Responses Tsupporting

confidence: 71%

Differential Expression of Smad7 Transcripts Identifies the CD4+CD45RChigh Regulatory T Cells That Mediate Type V Collagen-Induced Tolerance to Lung Allografts

Mizobuchi

Yasufuku

Zheng

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…29 Likewise, increases in CD8 ϩ cells correlate with the progression of acute rejection in a model of rat transplantation. 30 Taken together, these results suggest that aspiration of gastric contents leads to an increased potential for antigen presentation secondary to in-creases in graft APCs, as well as increased rejection injury as a result of more graft-infiltrating lymphocytes.…”

Section: Discussionmentioning

confidence: 75%

Chronic aspiration of gastric fluid accelerates pulmonary allograft dysfunction in a rat model of lung transplantation

Hartwig

Appel

et al. 2006

The Journal of Thoracic and Cardiovascular Surgery

View full text Add to dashboard Cite

show abstract

“…Recent work in a rat model of lung transplantation from the Duke group has shown that allografts challenged with chronic aspiration demonstrated severe grade 4 acute rejection with significant monocyte infiltration, fibrosis, and lung destruction (29). Aspiration was also associated with increases in CD8 ϩ T cells and this "proof of principle" study indicates that aspiration may indeed lead to pathological changes previously attributed to T-cell, alloimmune-based mechanisms (30,31).…”

Section: Discussionmentioning

confidence: 80%

Pepsin, a Biomarker of Gastric Aspiration in Lung Allografts

Stovold

Forrest²,

Corris³

et al. 2007

Am J Respir Crit Care Med

161

101

View full text Add to dashboard Cite

show abstract

Progressive increase of CD4+/CD45RC− lymphocytes after allograft rat lung transplantation: A marker of acute rejection

Abstract: Significant change of lymphocyte subpopulations is a sign of lymphocyte activation. Increased CD4(+)/CD45RC(-) cells in lung allografts could be an early marker of acute rejection, which can be examined by means of lung lavage and flow cytometry.

Cited by 7 publications

References 30 publications

Differential Expression of Smad7 Transcripts Identifies the CD4+CD45RChigh Regulatory T Cells That Mediate Type V Collagen-Induced Tolerance to Lung Allografts

Differential Expression of Smad7 Transcripts Identifies the CD4+CD45RChigh Regulatory T Cells That Mediate Type V Collagen-Induced Tolerance to Lung Allografts

Chronic aspiration of gastric fluid accelerates pulmonary allograft dysfunction in a rat model of lung transplantation

Pepsin, a Biomarker of Gastric Aspiration in Lung Allografts

Contact Info

Product

Resources

About