Progressive Control of Streptococcus agalactiae-Induced Innate Inflammatory Response Is Associated with Time Course Expression of MicroRNA-223 by Neutrophils

Abstract

Group B streptococcus (GBS) is a human pathogenic bacteria inducing a strong inflammatory response that may be detrimental for host tissues if not finely regulated. The inflammatory response can be modulated by different molecular mechanisms, among which growing evidences point toward the crucial role of microRNAs. Regarding innate inflammatory response, studies have reported that miR-223 is essential for the control of granulocyte proliferation and activation. Moreover, number of investigations on miRNA expression profiling performed in various inflammatory settings reveal that miR-223 is among the top differentially expressed miRNA. Yet, the dynamic pattern of expression of miR-223 in vivo with respect to the evolution of the inflammatory process, especially in microbial infection, remains elusive. In this study, we analyzed the kinetic expression of miR-223 in an inflammatory model of GBS-induced murine pneumoniae and looked for correlates with inflammatory markers including innate cell infiltrates. We found that miR-223 expression is rapidly induced at very early time points (3-6 h post infection) mainly by lung infiltrating neutrophils. Interestingly, the level of miR-223 accumulating in the lungs remains higher at later stages of infection (24h and 48h pi) and this correlates with reduced expression of primary inflammatory cytokines and chemokines and with a shift in infiltrating monocyte and macrophage subtypes toward regulatory phenotype. Transient inhibition of miR223 by an antagomir resulted in significant increase of CXCL2 expression and partial enhancing of infiltrating neutrophils in GBS infected lung tissues. This suggests the potential contribution of miR-223 to the resolution phase of GBS-induced acute inflammation.

Regulatory network of miRNA, lncRNA, transcription factor and target immune response genes in bovine mastitis

“…Ma et al 36 identified bta-miR-185 targeting five genes including MLLT3, DYRK1B and NPR2. Other studies have demonstrated the involvement of bta-miR-223 in bovine mastitis immune response 32 , 37 , and also in other immune responses during lung infection, bacterial peritonitis, and cutaneous leishmaniasis 38 – 41 . The other two miRNAs identified in our study, bta-mir-149-5p and bta-miR-874, have been associated with fat levels in beef cattle 42 , 43 .…”

Regulatory Network of miRNA, lncRNA, Transcription Factor and Target Immune Response Genes in Bovine Mastitis

“…Ma et al 36 identi ed bta-miR-185 targeting ve genes including MLLT3, DYRK1B and NPR2. Other studies have demonstrated the involvement of bta-miR-223 in bovine mastitis immune response 32,37 , but also in other immune responses to lung infection, bacterial peritonitis, and cutaneous leishmaniasis [38][39][40][41] . The other two miRNAs identi ed in our study, bta-mir-149-5p and bta-miR-874, have been associated with fat levels in beef cattle 42,43 .…”

miR-223: An Effective Regulator of Immune Cell Differentiation and Inflammation

“…Additionally, the lipopolysaccharide (LPS) challenge resulted in rapid accumulation of endotoxin, and the inflammatory lung pathology induction was characterized by excessive lung tissue injury 13 . During Group B Streptococcus (GBS)-induced lung inflammation, miR-223 was rapidly up-regulated in lung-infiltrating granulocytes at 3-6 h post-GBS infection and this attenuated lung tissue injury 41 .…”