Progressive Change of Optical Coherence Tomography Scans in Retinal Degeneration Slow Mice

Horio, Naoichi; Kachi, Syu; Hori, Kenji; Okamoto, Yoko; Yamamoto, Etsuko; Terasaki, Hiroko; Miyake, Yoichi

doi:10.1001/archopht.119.9.1329

Cited by 63 publications

(45 citation statements)

References 17 publications

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“…Reduced retinal thickness observed in OCT scan images of 21-day-old S334ter-line-3 rats is found to be well-correlated with histology data. Comparable observations have been reported in retinal degenerate (rd) mice (Horio et al, 2001;Li et al, 2001).…”

Section: Discussionsupporting

confidence: 65%

“…More recently, the technique has been used in animal models as a useful tool to assess native as well as iatrogenic retinal defects (Toth et al, 1997a,b;Ge et al, 1999;Ducros et al, 2001;Fukuchi et al, 2001;Horio et al, 2001; 2001; Gloesmann et al, 2003). Recent investigations in mice demonstrate the reliability of OCT imaging to measure retinal thickness at various stages of retinal degeneration (Horio et al, 2001;Li et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Retinal transplants evaluated by optical coherence tomography in photoreceptor degenerate rats

Thomas

Arai

Ikai

et al. 2006

Journal of Neuroscience Methods

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Optical coherence tomography (OCT), a non-invasive method, was used for qualitative assessment of fetal retinal sheet transplants by noninvasive imaging. Rhodopsin-mutant S334ter-line-3 rats with fast retinal degeneration (28-37-day old) were transplanted with fetal retinal sheets from embryonic day (E) 18-19 pigmented normal rats. Retinal thickness measurements from transplanted (n = 51), no surgery control (n = 8), and normal pigmented rat eyes (n = 6) were obtained using a Zeiss stratus OCT-3 scanning instrument. Frozen retinal sections were stained with hematoxylin/eosin. S334ter-line-3 rats showed significant reduction in OCT retinal thickness (p < 0.001) compared to normal pigmented rats at the age of 21 days. In 62% of the transplanted rats, OCT scanning revealed the presence of a subretinal graft, which was confirmed by subsequent histology. Retinal thickness in the transplant area was significantly increased compared to the area outside the transplant and to non-transplanted eyes (p < 0.001). While most of the transplants with single-band OCT images (87%) had rosetted transplants, a considerable proportion of transplants having a multi-band OCT image were found to have well-laminated areas in the graft after histological evaluation. Following retinal transplantation in rodents, OCT imaging data correlated mostly with transplant morphology. OCT is a useful technique for in vivo screening and evaluation of retinal transplants. This technique determines surgical outcomes at a much earlier stage.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Retinal transplants evaluated by optical coherence tomography in photoreceptor degenerate rats

Thomas

Arai

Ikai

et al. 2006

Journal of Neuroscience Methods

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“…For more than a decade, in vivo longitudinal imaging has been reliably performed in experimental rodent models of optic nerve damage causing RGC loss. Commonly used methods include fluorescence imaging of retrogradely labelled RGCs, 26,27 structural imaging of the optic nerve head [28][29][30][31] or axons, 32 and imaging of RGC apoptosis. 33 Of particular interest is the ability to image living RGC somas expressing fluorescent proteins.…”

Section: Tracking Retinal Ganglion Cells In Vivomentioning

confidence: 99%

Assessing retinal ganglion cell damage

Smith

Vianna

Chauhan

2017

Eye

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Retinal ganglion cell (RGC) loss is the hallmark of optic neuropathies, including glaucoma, where damage to RGC axons occurs at the level of the optic nerve head. In experimental glaucoma, damage is assessed at the axon level (in the retinal nerve fibre layer and optic nerve head) or at the soma level (in the retina). In clinical glaucoma where measurements are generally limited to non-invasive techniques, structural measurements of the retinal nerve fibre layer and optic nerve head, or functional measurements with perimetry provide surrogate estimates of RGC integrity. These surrogate measurements, while clinically useful, are several levels removed from estimating actual RGC loss. Advances in imaging, labelling techniques, and transgenic medicine are making enormous strides in experimental glaucoma, providing knowledge on the pathophysiology of glaucoma, its progression and testing new therapeutic avenues. Advances are also being made in functional imaging of RGCs. Future efforts will now be directed towards translating these advances to clinical care.

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“…[12][13][14][15][16] Furthermore, OCT images of rd animal models have a predictable relationship with histology and demonstrate potential for monitoring structural progression of the degenerative retina. [17][18][19] Multifocal electroretinography (mfERG) allows for the simultaneous recording of focal retinal responses and can be used to provide information about localised retinal function in RP. [20][21][22][23] In particular, mfERGs can be useful in monitoring macular cone system function in RP and indeed mfERG responses have been shown to be associated with the subjective visual field size.…”

Section: Introductionmentioning

confidence: 99%

The association between multifocal electroretinograms and OCT retinal thickness in retinitis pigmentosa patients with good visual acuity

Wolsley

Silvestri

O’Neill

et al. 2008

Eye

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Aims To investigate relationships between retinal morphology and retinal function in patients with retinitis pigmentosa (RP) using optical coherence tomography (OCT) and multifocal electroretinography (mfERG). Methods In all, 14 patients with RP who had visual acuities of 0.2 logMAR or better and Humphrey central fields of 101 or larger participated in the study along with 16 normal control subjects. The amplitudes and timings of the mfERG responses were compared with spatially corresponding measures of retinal layer thickness from OCT within the macula region (central 121). Results Eyes with RP showed thinning of the photoreceptor retinal (PR) layer and thickening of mid-inner retinal (MIR) layers beyond the fovea. mfERG amplitude was reduced in all regions, whereas mfERG timing was only significantly delayed at a retinal eccentricity of 6-121 and was otherwise preserved within the foveal and parafoveal retina (0-61). PR layer thickness was correlated with mfERG amplitude across the macula region. mfERG timing was correlated with the total change in retinal thickness (combined PR thinning and MIR thickening) at an eccentricity of 6-121. Conclusions The relationship between mfERG timing and retinal thickness in RP is dependent on the retinal eccentricity. Preserved timing in the central retina (0-61), despite significant disruption to retinal laminar structure, could be suggestive of inner retinal remodelling or functional redundancy.Cone system activity derived from mfERG amplitude appears to be related to the thickness of the photoreceptor layer in the macula region.

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Progressive Change of Optical Coherence Tomography Scans in Retinal Degeneration Slow Mice

Cited by 63 publications

References 17 publications

Retinal transplants evaluated by optical coherence tomography in photoreceptor degenerate rats

Retinal transplants evaluated by optical coherence tomography in photoreceptor degenerate rats

Assessing retinal ganglion cell damage

The association between multifocal electroretinograms and OCT retinal thickness in retinitis pigmentosa patients with good visual acuity

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