Progression of renal fibrosis in congenital CKD model rats with reduced number of nephrons

Yasuda, Hidenori; Tochigi, Yuki; Katayama, Kentaro; Suzuki, Hiroyoshi

doi:10.1016/j.etp.2017.01.007

Cited by 7 publications

(10 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Following three washes with PBS, tissue sections were blocked with 10% donkey serum (Sigma-Aldrich) in PBS for 30 min and incubated overnight at 4 °C with primary antibodies. After washing with PBS, the sections were incubated with appropriate secondary antibodies [55,56] for 1 h at room temperature. The resultant sections were mounted in ProLong Gold Antifade Reagent containing DAPI (Life Technologies, Carlsbad, CA, USA) for counterstaining.…”

Section: Methodsmentioning

confidence: 99%

Loss of Wwox Causes Defective Development of Cerebral Cortex with Hypomyelination in a Rat Model of Lethal Dwarfism with Epilepsy

Tochigi

Takamatsu

Nakane

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

WW domain-containing oxidoreductase (Wwox) is a putative tumor suppressor. Several germline mutations of Wwox have been associated with infant neurological disorders characterized by epilepsy, growth retardation, and early death. Less is known, however, about the pathological link between Wwox mutations and these disorders or the physiological role of Wwox in brain development. In this study, we examined age-related expression and histological localization of Wwox in forebrains as well as the effects of loss of function mutations in the Wwox gene in the immature cortex of a rat model of lethal dwarfism with epilepsy (lde/lde). Immunostaining revealed that Wwox is expressed in neurons, astrocytes, and oligodendrocytes. lde/lde cortices were characterized by a reduction in neurite growth without a reduced number of neurons, severe reduction in myelination with a reduced number of mature oligodendrocytes, and a reduction in cell populations of astrocytes and microglia. These results indicate that Wwox is essential for normal development of neurons and glial cells in the cerebral cortex.

show abstract

Section: Methodsmentioning

confidence: 99%

Loss of Wwox Causes Defective Development of Cerebral Cortex with Hypomyelination in a Rat Model of Lethal Dwarfism with Epilepsy

Tochigi

Takamatsu

Nakane

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…These processes result in classic histologic DKD kidney changes in humans and animals such as glomerulomegaly, 73 glomerulosclerosis, and mesangial expansion 74 that closely associate with DKD progression 75 . Summarily, kidney fibrosis, fueled by maladaptive inflammation, represents a common final pathway of all chronic kidney disease (CKD) 76,77 . Each of these pathways were captured in the current meta‐analysis which also emphasized effects on individual markers of injury (TGF‐β, collagen I, fibronectin, reactive oxidative species, TNF‐α, macrophage, and leucocyte infiltration) and repair (E‐cadherin, superoxide dismutase, IL‐10).…”

Section: Discussionmentioning

confidence: 99%

“…75 Summarily, kidney fibrosis, fueled by maladaptive inflammation, represents a common final pathway of all chronic kidney disease (CKD). 76,77 Each of these pathways were captured in the current meta-analysis which also emphasized effects on individual markers of injury (TGF-β, collagen I, fibronectin, reactive oxidative species, TNF-α, macrophage, and leucocyte infiltration) and repair (E-cadherin, superoxide dismutase, IL-10). This evidence synthesis suggested that cell-based therapy substantially improved injury while simultaneously promoting pro-repair activities in the kidney, blood, and urine in animals, therein targeting multiple pathogenic pathways in DKD.…”

Section: Publication Biasmentioning

confidence: 99%

A Systematic Review and Meta-Analysis of Cell-Based Interventions in Experimental Diabetic Kidney Disease

Hickson

Abedalqader

Ben-Bernard

et al. 2021

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Regenerative, cell-based therapy is a promising treatment option for diabetic kidney disease (DKD), which has no cure. To prepare for clinical translation, this systematic review and meta-analysis summarized the effect of cell-based interventions in DKD animal models and treatment-related factors modifying outcomes. Electronic databases were searched for original investigations applying cell-based therapy in diabetic animals with kidney endpoints (January 1998-May 2019). Weighted or standardized mean differences were estimated for kidney outcomes and pooled using random-effects models. Subgroup analyses tested treatment-related factor effects for outcomes (creatinine, urea, urine protein, fibrosis, and inflammation). In 40 studies (992 diabetic rodents), therapy included mesenchymal stem/stromal cells (MSC; 61%), umbilical cord/amniotic fluid cells (UC/AF; 15%), non-MSC (15%), and cell-derived products (13%). Tissue sources included bone marrow (BM; 65%), UC/AF (15%), adipose (9%), and others (11%). Cell-based therapy significantly improved kidney function while reducing injury markers (proteinuria, histology, fibrosis, inflammation, apoptosis, epithelial-mesenchymal-transition, oxidative stress). Preconditioning, xenotransplantation, and disease-source approaches were effective. MSC and UC/AF cells had greater effect on kidney function while cell products improved fibrosis. BM and UC/AF tissue sources more effectively improved kidney function and proteinuria vs adipose or other tissues. Cell dose, frequency, and administration route also imparted different benefits. In conclusion, cell-based interventions in diabetic animals improved kidney function and reduced injury with treatment-related factors Authored by a member of IFATS.

show abstract

“…Do ponto de vista renal, esses eventos contribuem para a hipertrofia glomerular e tubular, com perda gradativa da função renal (Yasuda et al, 2017). Evidências recentes apontam para a presença de RAGE no meio intracelular, mediando resposta biológica desencadeada por AGE gerados localmente (Sotokawauchi et al, 2019).…”

Section: Produtos De Glicação Avançada Na Doença Renal Crônicaunclassified

Enriquecimento da HDL em apolipoproteína A-IV favorece sua funcionalidade na doença renal diabética

Santana¹

View full text Add to dashboard Cite

Progression of renal fibrosis in congenital CKD model rats with reduced number of nephrons

Cited by 7 publications

References 44 publications

Loss of Wwox Causes Defective Development of Cerebral Cortex with Hypomyelination in a Rat Model of Lethal Dwarfism with Epilepsy

Loss of Wwox Causes Defective Development of Cerebral Cortex with Hypomyelination in a Rat Model of Lethal Dwarfism with Epilepsy

A Systematic Review and Meta-Analysis of Cell-Based Interventions in Experimental Diabetic Kidney Disease

Enriquecimento da HDL em apolipoproteína A-IV favorece sua funcionalidade na doença renal diabética

Contact Info

Product

Resources

About