Progression of Rare Inherited Retinal Dystrophies May Be Monitored by Adaptive Optics Imaging

Samelska, Katarzyna; Szaflik, Jacek Paweł; Śmigielska, Barbara; Zaleska-Żmijewska, Anna

doi:10.3390/life13091871

Cited by 3 publications

(3 citation statements)

References 35 publications

(59 reference statements)

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“…The image dimensions are 4 • × 4 • , representing 1.2 mm × 1.2 mm of the retina. Image acquisition in a single position lasts approximately 4 s, during which 40 individual images are acquired [22,24]. The Rtx1™ software provides the program AO Detect Artery to analyse retinal vessel parameters and correct distortions within frames [22,25].…”

Section: Examinationmentioning

confidence: 99%

Is Primary Open-Angle Glaucoma a Vascular Disease? Assessment of the Relationship between Retinal Arteriolar Morphology and Glaucoma Severity Using Adaptive Optics

Szewczuk,

Wawrzyniak,

Szaflik

et al. 2024

JCM

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Background: Retinal vascular abnormalities may be associated with glaucomatous damage. Adaptive optics (AO) is a new technology that enables the analysis of retinal vasculature at the cellular level in vivo. The purpose of this study was to evaluate retinal arteriolar parameters using the rtx1 adaptive optics fundus camera (AO-FC) in patients with primary open-angle glaucoma (POAG) at different stages and to investigate the relationship between these parameters and changes in spectral-domain optical coherence tomography (SD-OCT) and perimetry. Methods: Parameters of the retinal supratemporal and infratemporal arterioles (wall thickness (WT), lumen diameter (LD), total diameter (TD), wall-to-lumen ratio (WLR), and cross-sectional area of the vascular wall (WCSA)) were analysed with the rtx1 in 111 POAG eyes, which were divided into three groups according to the severity of the disease, and 70 healthy eyes. The associations between RTX1 values and the cup-to-disk ratio, SD-OCT parameters, and visual field parameters were assessed. Results: Compared with the control group, the POAG groups showed significantly smaller TD and LD values (p < 0.05) and significantly higher WLR and WT values (p < 0.05) for the supratemporal and infratemporal arterioles. TD was significantly positively correlated with the retinal nerve fibre layer (RNFL) and ganglion cell complex (GCC) (p < 0.05). LD was significantly positively correlated with the RNFL, GCC, and rim area (p < 0.05). The WLR was significantly negatively correlated with the RNFL, GCC, rim area, and MD (p < 0.05), while it was significantly positively correlated with the cup-to-disc ratio and PSD (p < 0.05). Conclusions: The results suggest that vascular dysfunction is present in POAG, even at a very early stage of glaucoma, and increases with the severity of the disease.

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Section: Examinationmentioning

confidence: 99%

Is Primary Open-Angle Glaucoma a Vascular Disease? Assessment of the Relationship between Retinal Arteriolar Morphology and Glaucoma Severity Using Adaptive Optics

Szewczuk,

Wawrzyniak,

Szaflik

et al. 2024

JCM

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“…The ability to evaluate the retina at a cellular level highlights new anatomical readouts that can be used to evaluate the ability of 2D and 3D cultures to mimic the retina. These parameters include cellular density, spacing, and regularity ( 124 , 125 ). These insights can further guide the development of organoid or retina-on-a-chip technologies for more accurate biological representation.…”

Section: Technology Hurdles and Future Directionsmentioning

confidence: 99%

Modeling inherited retinal diseases using human induced pluripotent stem cell derived photoreceptor cells and retinal pigment epithelial cells

Seah,

Goh,

Banerjee

et al. 2024

Front. Med.

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Since the discovery of induced pluripotent stem cell (iPSC) technology, there have been many attempts to create cellular models of inherited retinal diseases (IRDs) for investigation of pathogenic processes to facilitate target discovery and validation activities. Consistency remains key in determining the utility of these findings. Despite the importance of consistency, quality control metrics are still not widely used. In this review, a toolkit for harnessing iPSC technology to generate photoreceptor, retinal pigment epithelial cell, and organoid disease models is provided. Considerations while developing iPSC-derived IRD models such as iPSC origin, reprogramming methods, quality control metrics, control strategies, and differentiation protocols are discussed. Various iPSC IRD models are dissected and the scientific hurdles of iPSC-based disease modeling are discussed to provide an overview of current methods and future directions in this field.

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“…The image dimensions are 4∘ × 4∘, representing 1.2 mm × 1.2 mm of the retina. Image acquisition in a single position lasts approximately 4 s, during which 40 individual images are acquired [25]. The Rtx1™ software provides the program AO Detect Artery to analyse retinal vessel parameters and correct distortions within frames [26].…”

Section: Examinationmentioning

confidence: 99%

Is Primary Open-Angle Glaucoma a Vascular Disease? – Assessment of the Relationship of Retinal Arteriolar Morphology and Glaucoma Severity Using Adaptive Optics

Szewczuk,

Wawrzyniak,

Szaflik

et al. 2023

Preprint

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Background: Retinal vascular abnormalities may be associated with glaucomatous damage. Adaptive optics (AO) is a new technology that enables analysis of retinal vasculature at the cellular level in vivo. The purpose of this study is to evaluate retinal arteriolar parameters using the adaptive optics fundus camera (AO -FC) rtx1 in patients with primary open-angle glaucoma (POAG) at different stages and to investigate the relationship between these parameters and changes in optical coherence tomography (OCT) and perimetry. We wanted to assess whether vascular changes could be a cause or effect of glaucomatous damage. Methods: Parameters of retinal supratemporal and infratemporal arterioles (wall thickness (WT), lumen diameter (LD), total diameter (TD), wall-to-lumen ratio (WLR), and cross-sectional area of the vascular wall (WCSA)) were analysed with the rtx1 in 111 POAG eyes, divided into three groups according to the severity of disease and 70 healthy eyes in correlation of structural defects in retinal nerve fibre layer and visual fields defects. Results: Compared with the control group, the POAG groups showed significantly smaller TD and LD values (p<0,05) and significantly higher WLR and WT values (p<0,05) for supratemporal and infratemporal arterioles. Rtx1 parameters significantly correlated with OCT parameters (retinal nerve fibre layer, ganglion cell complex and rim area) and visual field parameters (mean deviation and pattern standard deviation) (p<0.05). Conclusion: The results suggest that vascular dysfunction may be involved in POAG pathogenesis and neurodegenerative damage, even at the very early stage of glaucoma, without visual field defects.

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Progression of Rare Inherited Retinal Dystrophies May Be Monitored by Adaptive Optics Imaging

Cited by 3 publications

References 35 publications

Is Primary Open-Angle Glaucoma a Vascular Disease? Assessment of the Relationship between Retinal Arteriolar Morphology and Glaucoma Severity Using Adaptive Optics

Is Primary Open-Angle Glaucoma a Vascular Disease? Assessment of the Relationship between Retinal Arteriolar Morphology and Glaucoma Severity Using Adaptive Optics

Modeling inherited retinal diseases using human induced pluripotent stem cell derived photoreceptor cells and retinal pigment epithelial cells

Is Primary Open-Angle Glaucoma a Vascular Disease? – Assessment of the Relationship of Retinal Arteriolar Morphology and Glaucoma Severity Using Adaptive Optics

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