Progress toward the Synthesis of Pochonin J

Jackson, Sydney N.; Pongdee, Rongson

doi:10.4236/ijoc.2019.92009

Cited by 4 publications

References 17 publications

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A DNA Extraction Method for Nondestructive Testing and Evaluation of Cotton Seeds (Gossypium L.)

Karaca,

Ince

2023

Biochem Genet

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Kernels of cotton provide lint and linter for textiles, oil and protein for food and feed. Cotton seed is formed following fertilization between an ovule and a pollen grain. The seed coat is maternal in origin, whereas the embryo and attached cotyledonary leaves are hybrids of parental lines. The extraction of genomic DNA from an ungerminated whole, a portion or mixed seeds are prerequisite in genetic and genomic studies of cotton. As far as our knowledge, there is only one method of nondescriptive DNA extraction from ungerminated cotton seeds without affecting the seed germination capability, but it has technical di culties and requires special equipment. Furthermore, the amount of DNA extracted using the published method is low and, therefore, it is only suitable for routine marker assisted selection studies. In this study, a modi ed CTAB DNA isolation protocol referred to as the CTAB-LiCl DNA extraction method was developed for single whole cotton seed, a portion of cotton seed and bulked cotton seeds. The CTAB-LiCl DNA extraction method was evaluated in ninety-six individuals of six different cotton cultivars along with two genetic standards of cotton, TM-1 (G. hirsutum L.), Pima 3-79 (G. barbadense L.), and several other plant species of different plant genera. Results revealed that this method produced high quality and amounts of DNA as con rmed by spectrophotometry, agarose gel, restriction enzyme digestion, polymerase chain reaction, and library production for next generation sequencing studies of whole genome bisul te sequencing. It does not require the use of liquid nitrogen, RNase, proteinase K, or betamercaptoethanol and can be completed in approximately 2 h. Small tissues of the chalaza ends of ungerminated cotton seeds could be used to obtain high quality and quantity of DNA ranging from 14 µg to 28 µg without affecting the seeds' germination ability, allowing marker-assisted selection before planting and owering.

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A DNA Extraction Method for Nondestructive Testing and Evaluation of Cotton Seeds (Gossypium L.)

Karaca,

Ince

2023

Biochem Genet

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Relationships between chemical composition, antioxidant activity and genetic analysis with ISSR markers in flower buds of caper plants (Capparis spinosa L.) of two subspecies spinosa and rupestris of Spanish cultivars

Grimalt

García‐Martínez

Carbonell

et al. 2021

Genet Resour Crop Evol

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Genetic diversity and variability between populations is essential for the long-term survival of plant species as well as their adaptation to different habitats. The Capparis spinosa L. has two subspecies in Spain, spinosa with stipules thorny and rupestris without them. In Spain, the subspecies used for its cultivation is spinosa, which is difficult to manipulate due to its stipules thorny. The capers, unripe fruits and tender shoots are used as food. The caper plant is a rich source of phenolic compounds, due to that many flavonoids have been found in different parts of caper plant and in high quantities, which indicates that it is a good source of functional compounds both as food and for nutraceutical applications. There are no published works on the differences in biochemical and functional compounds of both subspecies, so in this work 32 varieties have been genetically analyzed to know their subspecies. Afterwards, various biochemical and functional parameters have been analyzed to find out if they present differences between both subspecies. From the results of the biochemical and functional parameters studied, there are no difference between the spinosa and rupestis subspecies, in all the parameters studied, except chlorophylls. There was more difference between the results of the subspecies spinosa among them, than with the subspecies rupestris. For all this, it can be concluded that the rupestris subspecies that does not present stipules thorniness can be cultivated, instead of the spinosa subspecies that does present them, without losing functional or nutritional characteristics of the caper buds.

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A DNA extraction method for nondestructive testing and evaluation of cotton seeds (Gossypium L.)

Karaca

İnce

2023

Preprint

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Kernels of cotton provide lint and linter for textiles, oil and protein for food and feed. Cotton seed is formed following fertilization between an ovule and a pollen grain. The seed coat is maternal in origin, whereas the embryo and attached cotyledonary leaves are hybrids of parental lines. The extraction of genomic DNA from an ungerminated whole, a portion or mixed seeds are prerequisite in genetic and genomic studies of cotton. As far as our knowledge, there is only one method of nondescriptive DNA extraction from ungerminated cotton seeds without affecting the seed germination capability, but it has technical difficulties and requires special equipment. Furthermore, the amount of DNA extracted using the published method is low and, therefore, it is only suitable for routine marker assisted selection studies. In this study, a modified CTAB DNA isolation protocol referred to as the CTAB-LiCl DNA extraction method was developed for single whole cotton seed, a portion of cotton seed and bulked cotton seeds. The CTAB-LiCl DNA extraction method was evaluated in ninety-six individuals of six different cotton cultivars along with two genetic standards of cotton, TM-1 (G. hirsutum L.), Pima 3–79 (G. barbadense L.), and several other plant species of different plant genera. Results revealed that this method produced high quality and amounts of DNA as confirmed by spectrophotometry, agarose gel, restriction enzyme digestion, polymerase chain reaction, and library production for next generation sequencing studies of whole genome bisulfite sequencing. It does not require the use of liquid nitrogen, RNase, proteinase K, or beta-mercaptoethanol and can be completed in approximately 2 h. Small tissues of the chalaza ends of ungerminated cotton seeds could be used to obtain high quality and quantity of DNA ranging from 14 µg to 28 µg without affecting the seeds’ germination ability, allowing marker-assisted selection before planting and flowering.

show abstract

Progress toward the Synthesis of Pochonin J

Cited by 4 publications

References 17 publications

A DNA Extraction Method for Nondestructive Testing and Evaluation of Cotton Seeds (Gossypium L.)

A DNA Extraction Method for Nondestructive Testing and Evaluation of Cotton Seeds (Gossypium L.)

Relationships between chemical composition, antioxidant activity and genetic analysis with ISSR markers in flower buds of caper plants (Capparis spinosa L.) of two subspecies spinosa and rupestris of Spanish cultivars

A DNA extraction method for nondestructive testing and evaluation of cotton seeds (Gossypium L.)

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