Progress on dot-blot assay as a promising analytical tool: Detection from molecules to cells

Surti, Parini; Kim, Min Woo; Phan, Le Minh Tu; Kailasa, Suresh Kumar; Mungray, Arvind Kumar; Park, Jong Pil; Park, Tae Jung

doi:10.1016/j.trac.2022.116736

Cited by 19 publications

(15 citation statements)

References 132 publications

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“…Caspase-3 is a protease enzyme that plays a central role in the process of programmed cell death, or apoptosis. It is involved in cleaving a variety of cellular proteins, leading to the characteristic morphological changes of apoptosis, including DNA fragmentation and cell shrinkage [ 40 , 41 , 42 ]. Finally, cyclin-D1 is a key regulator of the G1 phase of the cell cycle and is involved in promoting progression through the cell cycle [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

Critical-Illness: Combined Effects of Colistin and Vasoactive Drugs: A Pilot Study

Stamatiou,

Vasilaki,

Tzini

et al. 2023

Antibiotics

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Colistin is often used as a last resort for treating multidrug-resistant infections, particularly in critically ill patients in intensive care units. Nonetheless, its side effects, including myopathy, require careful monitoring. Vasoconstrictive drugs are also used in intensive care to increase blood pressure and improve blood flow to vital organs, which can be compromised in critically ill patients. The exact mechanism of colistin-induced muscle toxicity is of significant interest due to its potential intensive-care clinical implications. Colistin alone or in combination with vasoconstrictive agents was administrated in non-septic and LPS-induced septic animals for 10 days. Histopathological evaluation of the gastrocnemius muscle and dot-blot protein tissue analysis were performed. Increased intramuscular area, de-organization of the muscle fibers and signs of myopathy were observed in colistin-treated animals. This effect was ameliorated in the presence of vasoconstrictive drugs. Administration of colistin to septic animals resulted in a decrease of AMPK and cyclin-D1 levels, while it had no effect on caspase 3 levels. Vasoconstrictive drugs’ administration reversed the effects of colistin on AMPK and cyclin D1 levels. Colistin’s effects on muscle depend on septic state and vasoconstriction presence, highlighting the need to consider these factors when administering it in critically ill patients.

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Section: Methodsmentioning

confidence: 99%

Critical-Illness: Combined Effects of Colistin and Vasoactive Drugs: A Pilot Study

Stamatiou,

Vasilaki,

Tzini

et al. 2023

Antibiotics

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“…Compared with traditional organic dyes, QDs have high photostability, larger Stokes shift, higher brightness, high fluorescence quantum yield, and longer fluorescence lifetime . Furthermore, QDs can be further enhanced in terms of stability and biocompatibility through surface ligand modification , and can be loaded with specific targeting motifs and therapeutic agents, providing more possibilities for their applications in medical imaging and therapy. − So far, the commonly used luminescent QDs are mostly II–VI semiconducting nanomaterials, such as Cd(S, Se, and Te) QDs, − types of which have been the subject of significant controversy in biological applications due to their heavy metal content. Thus, some biocompatibility and nontoxicity QDs have been increasingly applied in diagnosis and treatment in recent years, such as Zn(S, Se, Te) semiconductor QDs. − Therefore, developing a new type of quantum dot nanomaterials with low toxicity and utilizing precise fluorescence-guided imaging would be highly beneficial.…”

Section: Introductionmentioning

confidence: 99%

Peptide-Functionalized ZnSe:Mn Quantum Dots as Fluorescent Probes for Accurate Localization of Hidden Dental Lesion Sites

Xue

Dong

et al. 2023

ACS Appl. Nano Mater.

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Streptococcus mutans (S. mutans) is considered the primary bacteria responsible for dental caries, and specific recognition and fluorescence labeling can achieve accurate detection and early diagnosis of oral diseases such as dental caries. However, due to the weak stability of traditional imaging probes in solution and a lack of specific recognition functions, there are still significant challenges in fluorescent imaging detection of dental caries lesions. Herein, we report a peptide-QDs composite fluorescent probe that can achieve highperformance imaging of S. mutans and enable early diagnosis of the susceptible sites and hidden early carious lesions of dental caries. This fluorescent probe has high fluorescence stability, good biocompatibility, and low biotoxicity in physiological environments. It can specifically recognize and fluorescently label S. mutans, responsible for early dental caries, accurately locating dental lesion sites. Combined with visible fluorescence analysis, it is more convenient to screen for hidden dental lesions that might otherwise be ignored. Due to its low synthesis cost, stable storage, and simple imaging method, the peptide-QDs composite fluorescent probe can be widely used in routine dental examinations for accurate screening of dental diseases.

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“…[23][24][25] Recent years, advances in the synthesis of different morphologies of Ag nanostructures has offered significant applications in multidisciplinary research. [26][27][28] Further, quantum dots have been used as promising fluorescent reader for sensing of small organic molecule (HCHO) in food sample. [29] Lipoic acid (LA), a thiol-containing capping reagent that has seen significantly used in the synthesis of metal NPs due to the disulfide interaction with metal NPs surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…Further, spherical nanoparticles were produced by using sodium citrate, polyethylene glycol (PEG), sodium borohydride and ascorbic acid, [20–22] with the addition of surfactants [23–25] . Recent years, advances in the synthesis of different morphologies of Ag nanostructures has offered significant applications in multidisciplinary research [26–28] . Further, quantum dots have been used as promising fluorescent reader for sensing of small organic molecule (HCHO) in food sample [29] .…”

Section: Introductionmentioning

confidence: 99%

Lipoic Acid Functionalized Silver Nanoprisms for Colorimetric Sensing of γ‐Aminobutyric Acid, Selenite and Myoglobin

Yele,

Patel,

Park

et al. 2023

ChemistrySelect

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Silver nanoprisms (AgNPRs) are synthesized by silver nitrate through chemical reduction method using sodium borohydride and hydrogen peroxide. AgNPRs were functionalized with lipoic acid (LA) and successfully integrated with UV‐visible spectrophotometry for simultaneous detection of multiple biomarkers such as γ‐aminobutyric acid (GABA), selenite, and myoglobin. The as‐prepared LA‐AgNPRs exhibited surface plasmon resonance (SPR) characteristic peak at 660 nm. The SPR band of LA‐AgNPRs was blue‐shifted to 439, 390, and 479 nm upon the addition of GABA, selenite, and myoglobin, respectively. The limits of detection are 6.32, 0.35 and 20.40 nM for GABA, selenite, and myoglobin, respectively. The LA‐AgNPRs sensor displays potential prospect to detect three biomarkers GABA, selenite, and myoglobin in biofluid samples.

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Progress on dot-blot assay as a promising analytical tool: Detection from molecules to cells

Cited by 19 publications

References 132 publications

Critical-Illness: Combined Effects of Colistin and Vasoactive Drugs: A Pilot Study

Critical-Illness: Combined Effects of Colistin and Vasoactive Drugs: A Pilot Study

Peptide-Functionalized ZnSe:Mn Quantum Dots as Fluorescent Probes for Accurate Localization of Hidden Dental Lesion Sites

Lipoic Acid Functionalized Silver Nanoprisms for Colorimetric Sensing of γ‐Aminobutyric Acid, Selenite and Myoglobin

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