Progress of pancreatitis disease biomarker alpha amylase enzyme by new nano optical sensor

Attia, M. S.; Al‐Radadi, Najlaa S.

doi:10.1016/j.bios.2016.06.079

Cited by 37 publications

(26 citation statements)

References 47 publications

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“…Additionally, in order to validate the AIMD method discussed above, we repeated the exact same calculations reported above yet using 2‐Chloro‐4‐nitrophenol (CP), which was produced from the reaction of alpha‐amylase enzyme with 2‐chloro‐4‐nitrophenyl‐α‐galactosylmaltoside, a process used for the determination of the reactivity of alpha‐amylase . CP quenches the luminescence of TbCCE similar to NAL.…”

Section: Resultsmentioning

confidence: 99%

Terbium Crown‐Ether Complex as a Stable Photoprobe

Abdel‐Mottaleb

Abdullah

Attia

2019

Applied Organom Chemis

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Terbium ion‐4'carboxybenzo‐18crown‐6‐ether complex (TbCCE) photoprobe is a sensitive and stable luminescent photoprobe used to detect low (9.4 x10−9 mol L−1) concentrations of nalbuphine (NAL) in serum and pharmaceutical formulations. Here we discuss molecular modeling of the interacting species using DFT and TD‐DFT. The results reveal strong binding energy of TbCCE (−153.36 kJ/mol) and explain the results of Stern‐Volmer bimolecular quenching analysis due to the presence of NAL in the proximity (at 2.403 Å apart from Tb ion) of the emissive TbCCE. Ab Initio molecular dynamic (AIMD) simulations are used to explain the dynamical changes of TbCCE molecular kinetic energy in its S1 state induced by collision with NAL. The AIMD simulates collisions between interacting molecules, which are reflected in the observed quenching of the photoprobe. Kinetic energy changes during molecular motions in the S1 state of TbCCE in presence of NAL indicate energy transfer process leading to quenching starting at 57 fs at NAL‐TbCCE distance is ~ 1.6 Å. The excited state AIMD simulations carried out in this work suggest a new avenue for future research on luminescence quenching.

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Section: Resultsmentioning

confidence: 99%

Terbium Crown‐Ether Complex as a Stable Photoprobe

Abdel‐Mottaleb

Abdullah

Attia

2019

Applied Organom Chemis

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“…Preparation of Tb-acetyl acetone (Tb-ACAC) complex embedded in PMMA [13][14][15][16][17][18]. (I) 0.12 g of Tb-acetyl acetone (Tb-ACAC) complex was dissolved in 10 mL ethanol (II) 5 g of PMMA was dissolved in 25 mL chloroform (III) 5 mL of solution (i) was mixed well with continuous stirring for 5 min.…”

Section: General Proceduresmentioning

confidence: 99%

Spectrofluorometric Determination of Alpha Fetoprotein in different serum samples of Liver Cancer by Tb-acetyl acetone complex embedded in Polymethylmethacrylate optical sensor

2019

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A SIMPLE, precise and sensitive method in which, Tb-acetyl acetone (Tb-ACAC) complex embedded in polymethylmethacrylate (PMMA) is used for the early diagnosis of liver cancer. The diagnosis process depends on the assessment of the concentration of alpha fetoprotein (AFP) in the serum samples of different liver patients. The Tb-acetyl acetone (Tb-ACAC) embedded in PMMA has strong emission band at 545 nm after excitation at 350 nm in ethanol. The assessment of alpha fetoprotein (AFP) depends on the quenching of the emission band at 545 nm in ethanol by the alpha fetoprotein (AFP). The calibration plot was achieved over the concentration 1 -550 ng/mL with a correlation coefficient of 0.99 and a detection limit of 0.5 ng/mL. The method was used satisfactorily for the diagnosis of liver cancer in a number of serum samples collected from various patients and health state; healthy (≤ 10 ng/mL) and HCC (400-550 ng/mL).

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“…After the addition of different concentrations of (NAL) into the Tb 3+ -4'carboxybenzo-18crown-6-ether ion in DMF, the intensity of the characteristic peak at 545 nm of Tb -4'carboxybenzo-18crown-6-ether was quenched indicating that (NAL) quenches the energy of the complex Tb 3+-4'carboxybenzo-18crown-6-ether. The characteristic peaks of Tb 3+ ion appear at ( 5 D 4 → 7 F 6 =490 nm, 5 D 4 → 7 F 5 =545 nm, 5 D 4 → 7 F 4 = 590 nm, 5 D 4 → 7 F 3 =620 nm and 5 D 4 → 7 F 2 = 650 nm) [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40].…”

Section: Emission Spectramentioning

confidence: 99%

Tb -4'carboxybenzo-18crown-6-ether Photo Probe or the Assessment of Nalbuphin HCl in Serum and Pharmaceutical Formulations

2018

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T HE EFFICIENCY of excited-state interaction between Tb 3+ and the 4'carboxybenzo-18crown-6-ether (CCE) has been studied in different solvents and pH. The high luminescence intensity of Tb-complex in DMF at 545 nm was obtained. The photo physical properties of the green emissive Tb 3+ complex have been elucidated. The Tb-CCE was used as photo probe for the assessment ofNalbuphine HCl in the pharmaceutical amp and serum samples at pH 6.5 and λ ex = 285 nm with a linear range 5x10 -8 to 1.2x10 -6 mol L −1 ofNalbuphine HCl, correlation coefficient of 0.993 and detection limit of 9.4 x10 -9 mol L −1 .

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Progress of pancreatitis disease biomarker alpha amylase enzyme by new nano optical sensor

Cited by 37 publications

References 47 publications

Terbium Crown‐Ether Complex as a Stable Photoprobe

Terbium Crown‐Ether Complex as a Stable Photoprobe

Spectrofluorometric Determination of Alpha Fetoprotein in different serum samples of Liver Cancer by Tb-acetyl acetone complex embedded in Polymethylmethacrylate optical sensor

Tb -4'carboxybenzo-18crown-6-ether Photo Probe or the Assessment of Nalbuphin HCl in Serum and Pharmaceutical Formulations

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