Progress in Treatment and Prevention of Trichinellosis

Yr, Yu; Qi, Yuanlin

doi:10.4172/2332-0877.1000251

Cited by 11 publications

(15 citation statements)

References 19 publications

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“…T. spiralis (ISS534) was isolated from a pig in Nanyang, Henan Province, China, and preserved by serial passage in BALB/c mice after every 6–8 months. T. spiralis muscle larvae (ML) was recovered from BALB/c mice by 40 dpi (days post-infection) with a standardized HCl–pepsin digestion technique [ 22 ]. Adult worms (AL) were retrieved from the intestine at 6 dpi, and newborn larvae (NBL) were recovered from a female adult at 6 dpi from the RPMI-1640 culture media at 37 °C for 24 h as previously described by [ 23 , 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…In the adjuvant group EtOH-IN, BALB/c mice were administrated only adjuvant EtOH with the same protocol, and the control group was kept without any drug therapy. On day six of mifepristone (M100 ng/mL) treatment, all groups of mice have orally injected 500 T. spiralis (ML) with the help of gastric cannula [ 22 ]. Adult worms (AL) were collected from the intestine at 6 days post-infection (dpi) and adult worms (F-AL, M-AL) were separated using Axiovert Zeiss Microscope (25x Neo Plan objectives) and observed the phenotypic appearance, especially pregnancy, occurred or not using an inverted bright field microscope (Olympus, Shibuya, Japan).…”

Section: Methodsmentioning

confidence: 99%

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Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone

Aleem

Shi

et al. 2021

Vaccines

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Trichinellosis is a foodborne zoonotic disease caused by Trichinella spp., including Trichinella spiralis. In the present study, T. spiralis membrane-associated progesterone receptor component-2 (Ts-MAPRC2) gene was cloned and characterized using protein sequencing analysis. Furthermore, the expression, purification, immunoblot assay, binding ability with progesterone antibody, and immunofluorescence assay were performed. A direct effect of progesterone (P4) and mifepristone (RU486) on the Ts-MAPRC2 gene was determined using in vitro cell culture that showed different expression levels at all developmental stages (muscle larvae (ML), female adult worm (F-AL), male adult worm (M-AL), and newborn larvae (NBL)). Subsequently, the in vitro phenotypic effects of P4, RU486, and rTs-MAPRC2-Ab on F-AL and ML stages were measured. Later, the in vivo phenotypic effect and relative mRNA expression of mifepristone on the F-AL stage were studied. Our results revealed that the Ts-MAPRC2 gene is critical to maintaining pregnancy in the female adult worm (F-AL) of T. spiralis. The 300 ng/mL of P4 and 100 ng/mL of RU486 showed downregulation of the Ts-MAPRC2 gene in F-AL (p ≤ 0.05). This plays an important role in abortion and possibly decreases the worm burden of T. spiralis in the host. Only 30 ng/mL P4 showed significant upregulation in F-AL (p ≤ 0.05). The current study provides new insights regarding the antihormone (P4 and RU486) drug design and vaccine therapy of recombinant (rTs-MAPRC2) protein as well as their combined effects to control T. spiralis infection.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone

Aleem

Shi

et al. 2021

Vaccines

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“…Moreover, those drugs and other anti-helminthics are effective on the T. spiralis enteric stages, not the systemic ones (Sharma et al, 2015) due to the parasite's resistance and low drug absorption. Since the discovery or development of anti-trichinosis therapy is necessary to eliminate the parasite intestinal forms (Kocięcka, 2000;Yr and Yf, 2015), medicinal plants have been lately tested either as mixtures of various biologically active ingredients or alone.…”

Section: Advances In Animal Andmentioning

confidence: 99%

The Potency of Lepidium sativum and Commiphora molmol Extracts on Trichinella spiralis Stages and Host Interaction

Abuelenain¹,

Fahmy²,

El-Shennawy³

et al. 2021

AAVS

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“…Anthelmintics, primarily albendazole and mebendazole, are the principal drugs for the treatment of trichinellosis. Thiabendazole is less frequently used because of its side effects [ 36 ]. Diethylcarbamazine is an anthelmintic used to treat filarial infections [ 37 ]; it was used as single therapy in nine patients, and for continuation of benzimidazole treatment in 56 patients ( Table 4 ).…”

Section: Discussionmentioning

confidence: 99%

Retrospective Survey of Human Trichinellosis in a Romanian Infectious Diseases Hospital over a Thirty-Year Interval—The Never-Ending Story

Lupşe

Ionică²,

Flonta³

et al. 2023

Pathogens

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Trichinellosis remains a food-safety risk in Romania due to cultural traditions and food behavior. The aim of the present study was to evaluate the epidemiological, clinical and therapeutical data of all human trichinellosis cases in patients admitted to an Infectious Diseases Hospital from northwestern Romania during a thirty-year interval. Between 1 January 1988 and 31 December 2018, a total of 558 patients were hospitalized with the diagnosis of trichinellosis. The number of cases/year varied between 1 and 86. The source of infection was known for 524 patients, represented by domestic pig meat (n = 484; 92.37%) and wild boar (n = 40; 7.63%). Most patients (410; 73.48%) presented were part of family or group outbreaks. Demographical and clinical data of patients will be presented. Antiparasitic therapy was prescribed in 99.46% of cases, and corticosteroids were prescribed in 77.06% of patients. In total, 48 patients (8.6%) presented complications of trichinellosis: 44 for a single complication (neurological, cardiovascular or respiratory); the others multiple complications. Pregnancy was documented in five patients. No fatalities occurred during the study period. Although the number of hospitalized patients has decreased in the last years, trichinellosis still remains an important public health problem in northwestern Romania.

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Progress in Treatment and Prevention of Trichinellosis

Cited by 11 publications

References 19 publications

Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone

Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone

The Potency of Lepidium sativum and Commiphora molmol Extracts on Trichinella spiralis Stages and Host Interaction

Retrospective Survey of Human Trichinellosis in a Romanian Infectious Diseases Hospital over a Thirty-Year Interval—The Never-Ending Story

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