Progress in the use of genetic methods to study insect behavior outside Drosophila

Mansourian, Suzan; Fandino, Richard A.; Riabinina, Olena

doi:10.1016/j.cois.2019.08.001

Cited by 11 publications

(4 citation statements)

References 139 publications

(173 reference statements)

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“…Genome sequencing and comprehensive gene expression analyses have been conducted on many insect species (Ellegren, 2014;Oppenheim et al, 2015). Combined with the establishment of gene manipulation methods (Adli, 2018;Mello and Conte, 2004), the molecular and neural bases of insect behavior have been elucidated in various non-model insect species other than Drosophila (Mansourian et al, 2019;Sun et al, 2017;Walton et al, 2020). The European honey bee (Apis mellifera L.) is a well-known social insect, and its social behavior has been extensively studied for many years (Frisch et al, 1967;Seeley, 1995;Winston, 1987).…”

Section: Introductionmentioning

confidence: 99%

Analysis of antennal responses to motion stimuli in the honey bee by automated tracking using DeepLabCut

Kohno

Kamata

Kubo

2023

Preprint

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Considering recent developments in gene manipulation methods for honey bees, establishing simple, robust, and indoor assay systems which can analyze behavioral components in detail is important for the rise of honey bee behavioral genetics. We focused on the antennal movements of the honey bee, which are used for not only multimodal sensory perception but also interactions between individuals. We developed an experimental system for analyzing the antennal responses (ARs) of the honey bee using DeepLabCut, a markerless posture-tracking tool using deep learning. The tracking of antennal movements during the presentation of vertical (downward and upward) motion stimuli using DeepLabCut successfully detected the ARs reported in the previous studies, where bees tilted their antennae in the direction opposite to the motion stimuli. In addition, we successfully detected ARs in response to horizontal (forward and backward) motion stimuli. An investigation of the developmental maturation of honey bee ARs showed that ARs to motion stimuli were not detected in bees immediately after emergence but became detectable through post-emergence development in an experience-independent manner. Furthermore, unsupervised clustering analysis using multidimensional data created by processing tracking data using DeepLabCut classified antennal movements into different clusters, suggesting that data-driven behavioral classification can apply to AR paradigms. These results reveal novel AR to visual stimuli and developmental maturation of ARs and suggest the efficacy of data-driven analysis for behavioral classification in behavioral studies of the honey bee.

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Section: Introductionmentioning

confidence: 99%

Analysis of antennal responses to motion stimuli in the honey bee by automated tracking using DeepLabCut

Kohno

Kamata

Kubo

2023

Preprint

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“…However, these systems remain heterologous and may not confidently reflect the PR function in vivo. The recent development of genome editing tools and their application to insects now allows linking gene loss with specific phenotypes [15,16]. For instance, CRISPR-Cas9 has been largely applied to knock-out Orco in different insect species, revealing its primordial function in olfactory transduction, including sex pheromone sensing in moths [17][18][19], but also in the development and maintenance of AL structure, depending on the species.…”

Section: Introductionmentioning

confidence: 99%

Pheromone Receptor Knock-Out Affects Pheromone Detection and Brain Structure in a Moth

et al. 2022

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Sex pheromone receptors are crucial in insects for mate finding and contribute to species premating isolation. Many pheromone receptors have been functionally characterized, especially in moths, but loss of function studies are rare. Notably, the potential role of pheromone receptors in the development of the macroglomeruli in the antennal lobe (the brain structures processing pheromone signals) is not known. Here, we used CRISPR-Cas9 to knock-out the receptor for the major component of the sex pheromone of the noctuid moth Spodoptera littoralis, and investigated the resulting effects on electrophysiological responses of peripheral pheromone-sensitive neurons and on the structure of the macroglomeruli. We show that the inactivation of the receptor specifically affected the responses of the corresponding antennal neurons did not impact the number of macroglomeruli in the antennal lobe but reduced the size of the macroglomerulus processing input from neurons tuned to the main pheromone component. We suggest that this mutant neuroanatomical phenotype results from a lack of neuronal activity due to the absence of the pheromone receptor and potentially reduced neural connectivity between peripheral and antennal lobe neurons. This is the first evidence of the role of a moth pheromone receptor in macroglomerulus development and extends our knowledge of the different functions odorant receptors can have in insect neurodevelopment.

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“…In the last decades, several well-studied examples that shed light on genes and pathways underlying insect behavioural plasticity have been described [1][2][3][4][5]. In particular, single genes that affect the regulation of complex, yet distinct, behaviour patterns have been identified, suggesting that mutations altering their expression may vary the response to specific environmental stimuli generating behavioural plasticity [1,[3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

The foraging Gene Is Involved in the Presence of Wings and Explorative Behaviours in Parthenogenetic Females of the Aphid Myzus persicae

Mandrioli

Manicardi

2022

Life

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The foraging gene (for) encodes for a cyclic guanosine monophosphate (cGMP)-dependent protein kinase involved in behavioural plasticity in aphids and in other insects. In this paper, we analysed the complete for sequence in eight clones of the peach potato aphid Myzus persicae, reporting the presence of nonsense and frameshift mutations in three studied clones characterized by a reduced number of winged females and by the absence of exploratory behaviours. Quantitative PCR experiments evidenced similar results in clones possessing for genes with a conserved coding sequence, but low expression levels. The comparison of the for transcriptional level in Myzus persicae persicae and Myzus persicae nicotianae showed very different expression in the two studied M. p. nicotianae clones so that our data did not support a previous hypothesis suggesting that a differential for expression was related to ecological specialization of M. p. nicotianae. In view of its role in both the dispersal of winged females and exploratory behaviours, the screening of the for sequences could be useful for predicting invasions of cultivated areas by peach potato aphids.

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Progress in the use of genetic methods to study insect behavior outside Drosophila

Cited by 11 publications

References 139 publications

Analysis of antennal responses to motion stimuli in the honey bee by automated tracking using DeepLabCut

Analysis of antennal responses to motion stimuli in the honey bee by automated tracking using DeepLabCut

Pheromone Receptor Knock-Out Affects Pheromone Detection and Brain Structure in a Moth

The foraging Gene Is Involved in the Presence of Wings and Explorative Behaviours in Parthenogenetic Females of the Aphid Myzus persicae

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