Progress in the Study of Lymph Node Metastasis in Early-stage Cervical Cancer

Huang, Bangxing; Fang, Fang

doi:10.1007/s11596-018-1915-0

Cited by 41 publications

(40 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to latestage CC patients, most early-stage (IA2-IIA2) CC patients have a significantly increased survival time after surgery and chemoradiotherapy. However, approximately 10-30% of early-stage patients were found to have pelvic lymph node metastasis (PLNM), and some of the patients eventually experienced adverse outcomes [2]. In earlystage CC, patients with moderately high-risk factors, including large tumor size (> 2 cm), poor differentiation, special pathologic types, deep stromal invasion, lymphovascular space invasion (LVSI), PLNM and parametrial infiltration, usually have relatively shorter survival times [2,3].…”

mentioning

confidence: 99%

DSG2 expression is correlated with poor prognosis and promotes early-stage cervical cancer

Qin

Liao

et al. 2020

Cancer Cell Int

View full text Add to dashboard Cite

Background: The pathogenesis and developmental mechanism of early-stage (FIGO 2009 IA2-IIA2) cervical cancer (CC) remain unclear. Seeking novel molecular biomarkers based on The Cancer Genome Atlas (TCGA) will facilitate the understanding of CC pathogenesis and help evaluate early-stage CC prognosis. Methods: To identify prognosis-related genes in early-stage CC, we analyzed TCGA mRNA-seq data and clinical data by univariate Cox and Kaplan-Meier plotter analyses. Differential expression analysis identified upregulated genes in early-stage CC. Combined with the genes correlated with unfavorable prognosis, we selected desmoglein-2 (DSG2) for further investigation. To detect DSG2 expression in early-stage CC, we used immunohistochemistry (IHC), quantitative real-time PCR (qRT-PCR) and western blotting. The relationship between the expression of DSG2 and clinical features was analyzed by the Chi square test. Cox analysis was applied to assess the relationship between CC overall survival (OS) and risk factors. The correlations between DSG2 expression and CC cell line proliferation and migration were investigated with Cell Counting Kit-8 (CCK-8) and migration assays. Results: There were 416 prognosis-related genes in early-stage CC. DSG2, matrix metallopeptidase 1 (MMP1), carbonic anhydrase IX (CA9), homeobox A1 (HOXA1), and serine protease inhibitor B3 (SERPINB3) were upregulated in early-stage CC compared with adjacent noncancerous tissue (ANT) and correlated with unfavorable prognosis. Among them, DSG2 was most significantly correlated with patient survival. Coexpression analysis indicated that DSG2 was probably involved in cell division, positive regulation of transferase activity, positive regulation of cell migration, EGFR upregulation pathway and regulation of lymphangiogenesis. IHC, qRT-PCR and western blotting showed that DSG2 expression was higher in CC than in normal tissue. Significant correlations were identified between DSG2 expression and several aggressive clinical features, including pelvic lymph node metastasis (PLNM). Multivariate Cox analysis showed that DSG2 and PLNM were independent prognostic factors for OS. DSG2 knockdown inhibited CC cell proliferation and migration. Conclusions: DSG2 is a biomarker that promotes tumor proliferation and metastasis and is correlated with poor prognosis in early-stage CC.

show abstract

mentioning

confidence: 99%

DSG2 expression is correlated with poor prognosis and promotes early-stage cervical cancer

Qin

Liao

et al. 2020

Cancer Cell Int

View full text Add to dashboard Cite

show abstract

“…The detection of micrometastasis of lymph nodes requires highly specific biomarkers, such as cytokeratin, SCC and HPV. However, IHC or cytokeratin are considered less specific (3)(4)(5)(6). Dürst et al (13) demonstrated that HPV16-E6-E7-mRNA was more sensitive and specific than cytokeratin-(CK)19-mRNA for the detection of disseminated tumor cells in SLNs.…”

Section: Discussionmentioning

confidence: 99%

“…Lentz et al (3) used immunohistochemistry (IHC) to assess 3,106 lymph nodes from 132 patients with pathologically negative lymph nodes, and reported that micrometastases can be found in 15% patients with early stage cervical cancer who were considered as lymph node negative by conventional histologic analysis. This is due to the failure of traditional pathological methods to diagnose the micro-metastasis of lymph nodes (4), and the fact that detection of micro-metastasis of lymph nodes requires highly specific biomarkers, such as cytokeratin, squamous cell carcinoma antigen (SCC) and human papilloma virus (HPV) (5). However, IHC or cytokeratin are considered less specific (6,7).…”

Section: Introductionmentioning

confidence: 99%

Detection of high‑risk human papillomavirus DNA in sentinel lymph nodes of patients with cervical cancer

Lü

Nong

et al. 2020

Oncol Lett

View full text Add to dashboard Cite

The aim of the present study was to investigate the expression of human papillomavirus (HPV) DNA in sentinel lymph nodes (SLN) in patients with early-stage cervical cancer (CC). In addition, the present study compared the positive rate of SLNs metastasis detected by routine pathological examination, and investigated the value of HPV-DNA in the detection of early CC lymph node micrometastasis. Reverse transcription-quantitative PCR (RT-qPCR) was used in order to evaluate the HPV DNA detection in all CC samples [International Federation of Gynecology and Obstetrics (FIGO) stage IA2-IIA2]. The consistency of HPV-DNA was compared between primary lesions and SLNs. The positive rates of HPV-DNA were compared with pathological diagnosis of SLN metastasis, and the association between the positive expression of HPV-DNA in SLNs and the clinical and pathological parameters of patients with cervical cancer were analyzed. A total of 345 sentinel lymph nodes were detected in 100 patients with IA2-IIA2 CC. The positive rates of RT-qPCR and conventional histopathological detection of SLNs metastasis were 31.6% (109/345) and 12.8% (44/345), respectively (P<0.001). The positive expression of HPV-DNA in SLNs was associated with the clinical stage and tumor diameter (P<0.05), but not with patients' age, depth of cervical invasion, histological grade, lymphatic and vascular space invasion (LVSI), squamous cell carcinoma antigen (SCCAg) (P>0.05). The detection of HPV-DNA expression in pelvic lymph nodes of early CC may be used to improve the detection rate of micrometastasis, guide the postoperative adjuvant therapy more accurately and improve prognosis. Patients with positive HPV-DNA would require closer surveillance than those with negative HPV-DNA.

show abstract

“…Although complete uniform pelvic lymphadenectomy is the routine procedure, the total number of pelvic LNs (PLNs) harvested varies among patients. Lymphatic dissemination to the regional LNs has been suggested as the main route of metastasis in cervical cancer [5]. Therefore, complete removal of PLNs is essential to improve survival, as it may increase the likelihood of detecting occult metastatic lesions [6].…”

Section: Clinical Features That Affect the Number Of Pelvic Lymph Nodmentioning

confidence: 99%

Clinical features that affect the number of pelvic lymph nodes harvested in patients with cervical cancer stage IB1 to IIA2

et al. 2021

View full text Add to dashboard Cite

Objective To investigate clinical features that affect the number of pelvic lymph nodes (PLNs) harvested and prognostic significance of the number of PLNs removed in patients with stage IB1 to IIA2 cervical cancer. Methods Data from patients with cervical cancer whom underwent hysterectomy with PLN dissection between June 2004 and July 2015 were reviewed retrospectively. Data on clinicopathologic factors including age, height, and weight were collected. Data on the presence of PLN metastasis on imaging studies prior to surgery, number of PLNs harvested, and presence of metastasis in the harvested PLNs were retrieved from medical records. Clinical features associated with the number of PLNs harvested were analyzed. Disease-free survival (DFS) and overall survival (OS) according to the number of PLNs harvested were analyzed. Results During the study period, 210 patients were included. The height and weight of patients and preoperative positive positron emission tomography findings were significantly associated with a higher number of PLNs harvested. As a pathologic factor, larger tumor size was associated with a higher number of PLNs harvested. Furthermore, a higher number of PLNs harvested was associated with a higher number of metastatic PLNs and patients undergoing postoperative concurrent chemoradiation therapy. Patient height and tumor size were independent factors affecting the number of PLNs harvested in multivariate analysis. However, the number of PLNs harvested was not associated with DFS or OS. Conclusion The number of PLNs harvested during surgery was associated with patient height; however, this was not related to the prognosis of the disease.

show abstract

Progress in the Study of Lymph Node Metastasis in Early-stage Cervical Cancer

Cited by 41 publications

References 48 publications

DSG2 expression is correlated with poor prognosis and promotes early-stage cervical cancer

DSG2 expression is correlated with poor prognosis and promotes early-stage cervical cancer

Detection of high‑risk human papillomavirus DNA in sentinel lymph nodes of patients with cervical cancer

Clinical features that affect the number of pelvic lymph nodes harvested in patients with cervical cancer stage IB1 to IIA2

Contact Info

Product

Resources

About