Progress in the Production of Haploid Embryos, Plants and Doubled Haploids in Cucumber (C. Sativus L.) by Gamma Irradiated Pollen, in Turkey

Çağlar, Gülat; Abak, K.

doi:10.17660/actahortic.1999.492.43

Cited by 16 publications

(8 citation statements)

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“…Increasing the irradiation dose still further to 400 Gy caused declines in pollen germination of 35% in 'XPH5811'. Similar to our finding, Caglar & Abak (1997) subjected cucumber pollen to gamma irradiation doses of 300, 450, and 600 Gy and found that viability and germination were both decreased by 2% or 3% (Table 1). The decline in viability and germination ratios under low dosage may result from radiobiological injury.…”

Section: Resultssupporting

confidence: 88%

In vitroandin vivobehavior of gamma-irradiated tomato (Lycopersicon esculentum) pollen

Akbudak

Seniz

2009

New Zealand Journal of Crop and Horticultural Science

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Pollen viability and germination in vitro and in vivo were influenced by gamma irradiation for dosages of 0 (control), 100, 200, 300, and 400 Gy when this was applied to early and late trusses (1st-5th and 6th-10th) 4 times in a year (early spring, late spring, early autumn, and late autumn) in three cultivars of tomato (Lycopersicon esculentum) ('Gulle', 'Likya', and 'XPH5811'). The trial was carried out in 2003 and was repeated in 2004. Timing 1 was applied to flowers in trusses 1-5 (early spring, early autumn), and timing 2 to flowers in trusses 6-10 (late spring, late autumn). exposure of pollen to high gamma doses, 300 and 400 Gy, resulted in the lowest rates of pollen survival and germination. Time of year and cultivar also had affects on pollen viability and germination rates. Viability and germination following exposure to gamma irradiation were not affected by the position of the truss on the plants. In situ pollen tube growth was recorded for all radiation dosages, pollen tubes of irradiated pollen were shown to grow successfully through the style and to enter the ovules.

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Section: Resultssupporting

confidence: 88%

In vitroandin vivobehavior of gamma-irradiated tomato (Lycopersicon esculentum) pollen

Akbudak

Seniz

2009

New Zealand Journal of Crop and Horticultural Science

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“…The first reported success in obtaining haploid or DH melons was achieved by rescue of parthenogenetic embryos induced by pollination with irradiated pollen (Sauton and Dumas de Vaulx 1987). This was confirmed and investigated further by other authors working with melon and/or other cucurbit crops (melon: Sauton 1988;Cuny et al 1992Cuny et al , 1993Ficcadenti et al 1995;watermelon: Sari et al 1994;cucumber: Sauton 1989;Przyborowski 1994;Caglar and Abak 1999;Lotfi et al 1999;squash: Kurtar et al 2002). Use of the technique in applied breeding programs has been limited by the low percentage of haploid embryos induced, difficulty in detecting and excising these embryos, and the small numbers of haploid plantlets recovered.…”

Section: Introductionmentioning

confidence: 81%

“…Some of them have already produced fruits after In future, colchicine would best be applied to shoots in vitro prior to transfer to soil. Haploid plants derived from embryos can easily be propagated and maintained in vitro to obtain populations of clonal plants for treatment with colchicine (Caglar and Abak 1999). If desired, one or more untreated clones could also be transferred to soil for early evaluation.…”

Section: Colchicine Treatment Of Shoots From Haploid Plants Re-establmentioning

confidence: 99%

Production of haploid and doubled haploid plants of melon (Cucumis melo L.) for use in breeding for multiple virus resistance

et al. 2003

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We have developed improved procedures for recovery of haploid and doubled haploid (DH) melon plants, using hybrids derived from crosses of lines with multiple virus resistance. Seeds formed after pollination with irradiated pollen were cultured in liquid medium for 10 days before excision of the embryos for further culture. This made it easier to identify the seeds containing parthenogenetic embryos, thereby reducing the effort required and increasing the percentage of plants recovered. The plants obtained (approximately 175) were transferred to a greenhouse for evaluation. Three fertile lines were identified, and selfed seeds were obtained for evaluating virus resistance. Flow cytometry of leaf tissues showed that two of these lines were spontaneous DH and the third was a mixoploid containing haploid and diploid cells. The other plants remained sterile through the flowering stage. Flow cytometry of 20 sterile plants showed that all were haploid. Attempts to induce chromosome doubling by applying colchicine to greenhouse-grown plants were unsuccessful. Shoot tips from the haploid plants were used to establish new in vitro cultures. In vitro treatment of 167 micropropagated haploid shoots with colchicine produced 10 diploid plants as well as 100 mixoploid plants. Pollen from male flowers that formed in vitro on the colchicine-treated plants was examined. High percentages of viable pollen that stained with acetocarmine were found not only in the diploids but also in >60% of the plants scored as mixoploid or haploid by flow cytometry. Efficient recovery of DH from hybrid melon lines carrying combinations of important horticultural traits will be a valuable tool for melon breeders.

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“…The induction of parthenogenetic development of the egg cell by pollination with irradiated pollen followed by haploid embryo rescue was first successfully used to obtain haploid embryos of melon and cucumber over 20 years ago (Sauton and Dumas de Vaulx 1987, Truong-Andre 1988, Niemirowicz-Szczytt and Dumas de Vaulx 1989. During the subsequent years, research was focused on the effect of the genotype, time of the year and radiation dose on the number of obtained haploids (Cuny et al 1993, Przyborowski and Niemirowicz-Szczytt 1994, Çağlar and Abak 1996a, 1999a, b, Faris et al 1999, Lofti et al 1999. Haploid plants of watermelon and summer squash were also obtained using irradiated pollen (Gürsöz et al 1991, Sari et al 1994, Kurtar et al 2002.…”

Section: Pollination With Irradiated Pollenmentioning

confidence: 99%

“…A low dose of gamma radiation, typically about 300 Gy, was shown to be effective in experiments carried out on melon (Sauton 1988), and was also optimal in cucumber haploid production (Niemirowicz-Szczytt et al 1995, Çağlar and Abak 1996a, 1999a. examined in detail the effect of the radiation dose on haploid embryo development using five lines and three hybrids of cucumber.…”

Section: Irradiation and Pollinationmentioning

confidence: 99%

Review of research on haploid production in cucumber and other cucurbits

Gałazka

Niemirowicz-Szczytt

2013

Folia Horticulturae

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This review provides a summary of haploid induction methods and factors affecting the efficacy of specific methodologies as applied to cucumber (Cucumis sativus L.), melon (Cucumis melo L.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai), winter squash (Cucurbita maxima Duch. ex Lam.), summer squash (Cucurbita pepo L.) and other cucurbits. This report is focused on studies that were carried out during the last 20 years. The main objective of the research on the production of haploid cucurbit plants is to accelerate breeding programs through the use of homozygous double haploid lines (DHL) and to facilitate the selection of desired (e.g. disease-resistant) genotypes for breeding. Unfortunately, currently used protocols result in a low number of double haploids (DH). The most common and best-known method of obtaining haploid cucurbit plants is via pollination with irradiated pollen, which induces parthenogenetic development of haploid embryos in planta. The embryos are extracted from immature seeds and cultured in vitro to facilitate the maturation and development of plants. The studies described below were primarily aimed at the determination of an appropriate dose of radiation and the evaluation of the impact of the genotype and the time of year on the number of haploid embryos and plants obtained. A less popular method of haploid production - ovule and ovary culture - is based on in vitro gynogenesis. The studies related to this method concentrated on optimising the composition of the medium and pre-treatment conditions (primarily temperature) to which the flower buds were subjected. Recently, increasing attention has been paid to anther and microspore culture. As in the case of in vitro ovule and ovary culture, the medium composition and flower bud pre-treatment conditions were optimised. The most recent studies suggest that anther culture is comparable in effectiveness to the irradiated pollen technique.

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Progress in the Production of Haploid Embryos, Plants and Doubled Haploids in Cucumber (C. Sativus L.) by Gamma Irradiated Pollen, in Turkey

Cited by 16 publications

References 0 publications

In vitroandin vivobehavior of gamma-irradiated tomato (Lycopersicon esculentum) pollen

In vitroandin vivobehavior of gamma-irradiated tomato (Lycopersicon esculentum) pollen

Production of haploid and doubled haploid plants of melon (Cucumis melo L.) for use in breeding for multiple virus resistance

Review of research on haploid production in cucumber and other cucurbits

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