Progress in the photodynamic therapy treatment of Leishmaniasis

Vital-Fujii, D.G.; Baptista, Maurı́cio S.

doi:10.1590/1414-431x2021e11570

Cited by 7 publications

(3 citation statements)

References 66 publications

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“…It should be noted that this low toxicity of ICG was also reported by other researchers (17,40). On the other hand, one of the main advantages of ICG over porphyrins is its minimal absorption in the 400-600 nm range, which results in lower phototoxicity on the skin (41).…”

Section: Discussionsupporting

confidence: 80%

Photo-induced antileishmanial activity of indocyanine green: In vitro and in vivo studies

Jalili,

Sazgarnia,

Hoseini

2023

Preprint

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Background Indocyanine green is a promising dye for photodynamic and photothermal therapy. However, ICG tends to aggregate in aqueous media, which limits its use in light therapy. Loading ICG onto a biocompatible structure can improve its aqueous stability. The aim of this study was to investigate the synergistic effect of PDT/PTT on leishmanial activity in the presence of micelles loaded with ICG. Methods After synthesizing micelles containing ICG, the dark toxicity of the pharmaceutical agents and in vitro phototoxicity by a cw 808 nm laser on promastigotes were determined via MTS assay. Finally, the efficacy of the treatments was assessed by measuring the diameter of the lesion every three days in a study conducted on 33 female BALB/c mice aged 4-6 weeks. Results At 808 nm, the absorbance of ICG inside the micelles was approximately2.5 times that of free ICG. The optimal concentration of ICG was determined to be 100 μM based on the toxicity of the medicinal agents topromastigotes. In the in vitro experiment, the groups containing ICG showed a significant decrease in survival compared to the control group with increasing light dose. In the animal model study, the simultaneous presence of medicinal agents and the application of a laser created a significant difference in the relative area of the lesion compared to the control group. Conclusion The findings of this study show that PTT/PDT mediated by ICG can be considered an inexpensive, safe, easy to administer and efficient treatment against Leishmania L. major both in vitro and in vivo. Moreover, this treatment does not cause any adverse effects when compared to other treatments.

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Section: Discussionsupporting

confidence: 80%

Photo-induced antileishmanial activity of indocyanine green: In vitro and in vivo studies

Jalili,

Sazgarnia,

Hoseini

2023

Preprint

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“…Since antimicrobial photodynamic therapy (aPDT) was first applied over a century ago, there have been ever-expanding number of studies in the literature that demonstrate the feasibility and efficacy of aPDT against several pathogenic microbes of clinical importance [ 59 , 60 , 61 , 62 , 63 , 64 , 65 ]. Within the aPDT field, many photosensitizers and wavelengths have been applied to harness their antimicrobial potential.…”

Section: Light-based Anti-infective Agents: Can They Overcome Antimic...mentioning

confidence: 99%

Antimicrobial Resistance: Is There a ‘Light’ at the End of the Tunnel?

Leanse,

Marasini,

dos Anjos

et al. 2023

Antibiotics

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In recent years, with the increases in microorganisms that express a multitude of antimicrobial resistance (AMR) mechanisms, the threat of antimicrobial resistance in the global population has reached critical levels. The introduction of the COVID-19 pandemic has further contributed to the influx of infections caused by multidrug-resistant organisms (MDROs), which has placed significant pressure on healthcare systems. For over a century, the potential for light-based approaches targeted at combatting both cancer and infectious diseases has been proposed. They offer effective killing of microbial pathogens, regardless of AMR status, and have not typically been associated with high propensities of resistance development. To that end, the goal of this review is to describe the different mechanisms that drive AMR, including intrinsic, phenotypic, and acquired resistance mechanisms. Additionally, the different light-based approaches, including antimicrobial photodynamic therapy (aPDT), antimicrobial blue light (aBL), and ultraviolet (UV) light, will be discussed as potential alternatives or adjunct therapies with conventional antimicrobials. Lastly, we will evaluate the feasibility and requirements associated with integration of light-based approaches into the clinical pipeline.

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“…The singlet oxygen species and/or free radicals generated upon illumination of this agent are toxic to pathogens (Sharma et al, 1999). The photodynamic effect may hit different targets, such as tumour tissues (Bonnett, 2002;Dolmans et al, 2003;Gunaydin et al, 2021), bacteria (Hamblin, Hasan, 2004;Liu et al, 2015;Martins Antunes de Melo et al, 2021), viruses and parasites (Fonseca et al, 2018;Varzandeh et al, 2021;Vital-Fujii, Baptista, 2021).…”

Section: Introductionmentioning

confidence: 99%

The effect of rose bengal activated with green diode laser light on selected Gram-positive and Gram-negative bacterial strains

Greczek-Stachura,

Różanowski,

Kania

2023

AUPCStudiaNaturae

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In recent years the photodynamic activity of rose bengal activated with green light against selected bacterial strains has been reported. However, according to our knowledge, the differences between the sensitivity of Gram-positive and Gram-negative bacterial strains in the presence of this photosensitizer have not been described. The aim of the conducted research was to examine the antibacterial effect of 535 nm wavelength diode laser light in the presence of rose bengal as photosensitizer on selected reference bacterial strains: Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus. Sterile 96-well microtiter plates were used to determine the antibacterial activity of the green light and rose bengal solutions at various concentrations. The labelled bacterial suspensions were placed to each well of the 96-well microtiter plate filled with liquid medium LB and solution of rose bengal. The plates were exposed to green diode laser light. After 24 hours of incubation at 37oC, the turbidance was read in a spectrophotometer. The irradiation in the presence of photosensitizer can act in an antibacterial manner, either bacteriostatically or bactericidally. The tested strains exhibit different sensitivity to irradiation because of the structure of the cell wall, the presence of different bacterial pigments and photoreceptor proteins in some species of bacteria. Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were the most photosensitive strains due to the higher possibility of rose bengal penetration into the bacterial cell, leading to the bacteriostatic effect. Our results show that rose bengal may be applied in the treatment of Gram-positive infections.

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Progress in the photodynamic therapy treatment of Leishmaniasis

Cited by 7 publications

References 66 publications

Photo-induced antileishmanial activity of indocyanine green: In vitro and in vivo studies

Photo-induced antileishmanial activity of indocyanine green: In vitro and in vivo studies

Antimicrobial Resistance: Is There a ‘Light’ at the End of the Tunnel?

The effect of rose bengal activated with green diode laser light on selected Gram-positive and Gram-negative bacterial strains

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