Progress in the analysis of brassinosteroids

Pan, Jialiang; Tan, Wei; Li, Gongke; Hu, Yuling

doi:10.3724/sp.j.1123.2011.00105

Cited by 4 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Brassinolides have no suitable chromophore for detection, and hence they are often derivatized with reagents to make them responsive to ultraviolet, fluorimetric, or electrochemical detectors. Several boronic acid reagents react with the vicinal diol groups have been used as derivatization reagents (Pan et al 2011), including naphthaleneboronic acid for ultraviolet detection (Gamoh et al 1988), 9-phenanthreneboronic acid (Gamoh et al 1989) and dansyl-3-aminophenylboronic acid ) for fluorimetric detection, and ferroceneboronic acid for electrochemical detection. High-performance liquid chromatography-mass spectroscopy (HPLC-MS) methods for analyzing brassinolides as their boronates have also been developed using atmospheric pressure chemical ionization (APCI) (Gamoh et al 1996) or electrospray ionization (ESI) (Svato s s, Antonchick, and Schneider 2004; Huo et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Determination of Brassinolide Analogs by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

Cao

Yang

et al. 2014

Analytical Letters

View full text Add to dashboard Cite

An efficient method based on high-performance liquid chromatography with evaporative light scattering detection was developed for the separation and determination of four brassinolide analogs [24-epibrassinolide, (22S, 23S)-24-epibrassinolide, 28-homobrassinolide, and (22S, 23S)-28-epihomobrassinolide] without prior derivatization. The optimized analysis was carried out on a C 18 reversed-phase column (150 mm Â 4.60 mm, 3 lm) at 30 C using isocratic elution of acetonitrile and water (38:62, v=v). The drift tube temperature of the detector was 60 C and the auxiliary gas (nitrogen) pressure was 360 kPa. The regression equations revealed linear relationships (R 2 ¼ 0.9984-0.9994) within the test ranges. The limits of detection and quantification were in the ranges of 0.12 to 0.17 lg and 0.24 to 0.33 lg, respectively. The fully validated method was applied to quantify the active ingredient content in technical material and formulations and provides an alternative approach for quality control.

show abstract

Section: Introductionmentioning

confidence: 99%

Determination of Brassinolide Analogs by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

Cao

Yang

et al. 2014

Analytical Letters

View full text Add to dashboard Cite

show abstract

“…The alcoholic extracts of E. scandens also exhibit low acute toxicity and exert signi cant inhibitory and protective effects in both rat and mouse models of hyperuricemia. Therefore, this species is an excellent potential alternative for the treatment and prevention of hyperuricemia (Pan et al 2020;Huang et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Evaluating the genetic diversity of Erythropalum scandens based on using inter-simple sequence repeat markers

Yang

Zhang

Huang

et al. 2023

Genet Resour Crop Evol

View full text Add to dashboard Cite

Erythropalum scandens Blume, an emerging medicinal plant with great potential for drug development, also possesses high edible value. In this study, we investigated the genetic diversity of the germplasm of E. scandens obtained from different geographical locations using inter simple sequence repeat (ISSR) markers. For this purpose, 18 ISSR primer pairs with a distinct background and adequate polymorphism were selected. We established an optimal ISSR-PCR reaction system (20 µL) with the following parameters: 1 µL DNA template (60 ng•µL -1 ), 1.2 µL primers (10 µmol•µL -1 ), 10 µL MasterMix, and 7.8 µL H 2 O. A total of 183 loci were ampli ed using the 18 primer pairs, of which 121 (66.12%) indicated polymorphism. Moreover, 34 germplasms of E. scandens exhibited genetic similarity coe cients ranging from 0.7104 to 0.9563, genetic distances ranging from 0.0447 to 0.3420, Nei's genetic diversity index of 0.1946, and Shannon's information index of 0.2982, suggesting high intraspeci c genetic diversity. UPGMA cluster and PCoA analyses distinguished the germplasm of E. scandens obtained from Guangxi from those collected from Guangdong, Hainan, Fujian, and Guizhou. However, the Mantel correlation analysis revealed that the genetic variation among the 34 germplasms of E. scandens was not signi cantly related to geographical distance. The analysis of the genetic background of wild and cultivated germplasms of E. scandens can help guide variety selection and breeding. Furthermore, the present study revealed the genetic background and a nities among 34 germplasms of E. scandens. Overall, our ndings lay the foundation for the conservation and utilization of germplasm resources, identi cation and classi cation of varieties, and variety selection and improvement of E. scandens at the molecular level. are shown in Fig. 2.

show abstract

“…However, the absence of suitable chromophores brings about problems in their detection. Therefore, BLs are often derivatized with pre-labeling reagents to make them responsive to ultraviolet, fluorimetric, or electrochemical detectors [ 8 , 9 ]. For this purpose, diverse boronic acid derivatization reagents have been used to condensed with the vicinal diol groups in BLs to produce the corresponding boronates, which exhibit strong absorption to facilitate extraction and detection.…”

Section: Introductionmentioning

confidence: 99%

Determination of Propionylbrassinolide and Its Impurities by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

Cao

Zhang

Zhang³

et al. 2018

Molecules

View full text Add to dashboard Cite

The discovery of brassinolide in 1979, a milestone in brassinosteroids research, has sparked great interest of brassinolide analogs (BLs) in agricultural applications. Among these BLs, propionylbrassinolide has captured considerable attention because it shows plant growth regulating activity with an excellent durability. Two impurities of propionylbrassinolide were isolated and purified by semi-preparative high-performance liquid chromatography (HPLC), and the chemical structures were confirmed. For simultaneous separation and determination of propionylbrassinolide and impurities, an efficient analytical method based on HPLC with evaporative light scattering detector (HPLC-ELSD) was developed. The optimized analysis was performed on a C18 reversed phase column (250 mm × 4.60 mm, 5 μm) with isocratic elution of acetonitrile and water (90:10, v/v) as the mobile phase. The drift tube temperature of the ELSD system was set to 50 °C and the auxiliary gas pressure was 150 kPa. The regression equations demonstrated a good linear relationship (R2 = 0.9989–0.9999) within the test ranges. The limits of detection (LODs) and quantification (LOQs) for propionylbrassinolide, impurity 1 and 2 were 1.3, 1.2, 1,3 and 4.3, 4.0, 4.2 mg/L, respectively. The fully validated HPLC-ELSD method was readily applied to quantify the active ingredient and impurities in propionylbrassinolide technical concentrate. Moreover, the optimized separation conditions with ELSD have been successfully transferred to mass spectrometry (MS) detector for LC-MS determination.

show abstract

Progress in the analysis of brassinosteroids

Cited by 4 publications

References 31 publications

Determination of Brassinolide Analogs by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

Determination of Brassinolide Analogs by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

Evaluating the genetic diversity of Erythropalum scandens based on using inter-simple sequence repeat markers

Determination of Propionylbrassinolide and Its Impurities by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

Contact Info

Product

Resources

About