Progress in Metabolic Engineering of
            <i>Saccharomyces cerevisiae</i>

Nevoigt, Elke

doi:10.1128/mmbr.00025-07

Cited by 526 publications

(348 citation statements)

References 416 publications

Supporting

Mentioning

333

Contrasting

Unclassified

Order By: Relevance

“…However, wild-type S. cerevisiae strains are unable to metabolize pentoses (C5 sugars), rendering the utilization of pentoses a significant challenge in biomass-to-ethanol conversion. Considerable research has been devoted to developing genetically modified organisms that co-ferment C6 sugars (hexoses) and C5 sugars to ethanol at high yields [2][3][4][5][6], which has led to commercial-scale co-fermentation being implemented at Poet-DSM's cellulosic ethanol plant in Emmetsburg, Iowa, and the Beta Renewables plant in Crescentino.…”

Section: Introductionmentioning

confidence: 99%

Combined production of biogas and ethanol at high solids loading from wheat straw impregnated with acetic acid: experimental study and techno-economic evaluation

Joelsson

Dienes

Kovács

et al. 2016

Sustain Chem Process

View full text Add to dashboard Cite

Background: Production of ethanol and biogas from acetic acid-impregnated steam-pretreated wheat straw was investigated. The solid fraction after pretreatment was used at high solids concentrations to generate ethanol by simultaneous saccharification and fermentation (SSF). The residual streams were evaluated with regard to biogas production. The experimental results were used to perform a techno-economic evaluation of a biorefinery concerning ethanol, raw biogas, pellet, and electricity production at various solid contents and residence times in the fermentation step. The configurations were also altered to include biogas upgrading to vehicle fuel quality or fermentation of xylose to ethanol. Results:At a water-insoluble solids (WIS) content in the SSF of between 10 and 20 %, the ethanol yields exceeded 80 %, the highest being 86 % at 12.5 % WIS, expressed as % of the theoretical maximum, based on the glucan content in SSF. Anaerobic digestion of wheat straw hydrolysate and stillage yielded 4.8 and 1.0-1.2 g methane/100 g dry straw, respectively, in 7-day experiments. Maximum recovery of overall product was achieved when SSF was run at between 10 and 15 % initial WIS content, for which the product yields from 100 g dry wheat straw were 16.1-16.3 g ethanol, 5.8-6.0 g methane, and 25 g lignin-rich solid residue. The net present value (NPV) was negative at discount rate of 11 % but positive at 5 % discount rate for all configurations. The 20 % WIS configuration with a residence time of 96 h in the fermentation stage attained the highest NPV. The minimum ethanol selling price varied between 0.72 and 0.87 EUR/L ethanol when the biogas was unchanged and it decreased to between 0.46 and 0.60 EUR/L ethanol when the biogas was upgraded to vehicle fuel quality or when xylose was converted to ethanol. Conclusions:According to the techno-economic assessment, a process that is based on the fermentation of only hexoses to ethanol, and production of raw biogas from xylose is not profitable under the economic assumptions including 11 % discount rate in the evaluation. However, the profitability of a plant can be improved by biogas upgrading to vehicle fuel quality or fermentation of xylose to ethanol.

show abstract

Section: Introductionmentioning

confidence: 99%

Combined production of biogas and ethanol at high solids loading from wheat straw impregnated with acetic acid: experimental study and techno-economic evaluation

Joelsson

Dienes

Kovács

et al. 2016

Sustain Chem Process

View full text Add to dashboard Cite

show abstract

“…Akibatnya biaya keseluruhan untuk produksi etanol G2 masih jauh lebih mahal daripada biaya produksi etanol generasi pertama (G1) yang bahan bakunya sama dengan bahan pangan. Hal tersebut memacu perkembangan rekayasa genetika, baik untuk memproduksi enzim maupun untuk menghasilkan mikroba unggul, seperti Saccharomyces cerevisiae, yang berkemampuan lebih terutama dalam hal enzimatiknya maupun toleransinya terhadap etanol (Alper et al, 2006;CarleUrioste et al, 1996;Nevoigt, 2008;Stephanopoulos, 2007).…”

Section: Pendahuluanunclassified

Kloning gen penyandi β-1,6-glukanase kapang secara cepat dengan teknik RT-PCR menggunakan primer spesifik Rapid cloning for gene encoding fungal β-1,6-glucanase by means of RT-PCR using specific primers

BUDIANI¹,

Putranto²,

MINARSIH³

et al. 2016

View full text Add to dashboard Cite

Production of bioethanol from biomass ofagricultural waste has been hindered with a highproduction cost because enzymes needed for theprocess has to be imported with relatively a highprice. Genetic engineering using its encodinggenes is able to produce those enzymes withlower cost. In this report we described a researchaimed to clone gene encoding β-1,6-glucanasefrom Trichoderma harzianum with a relativelyrapid and inexpensive method, by means of RT-PCR using gene specific primers. The primerswere designed based on the DNA sequence of thetarget gene from the same species of organismused in this research. RT-PCR using that primersresulted in DNA fragment with sizescorresponding to the predicted size of full lengthgene encoding β-1,6-glucanase, about 1300 bp.After a sequential experiments of cloning usingpGEM-T Easy vector, DNA sequencing andBlastN - BlastX analyses of the sequences, it wasproven that the isolated DNA was full length geneof β-1,6-glucanase. This was implied from thepercentage of Identity and E-value which were96% and 0.0 (< e-04) respectivety.AbstrakProduksi bioetanol dari biomassa limbahpertanian, terkendala oleh tingginya biayaproduksi karena enzim yang diperlukan untukproses tersebut masih harus diimpor denganharga yang relatif mahal. Melalui rekayasagenetika menggunakan gen-gen penyandinya,enzim-enzim tersebut dapat diproduksi denganbiaya yang lebih murah. Penelitian ini bertujuanuntuk mengklon gen penyandi β-1,6-glukanasedari Trichoderma harzianum secara cepat danekonomis, dengan RT-PCR menggunakan primerspesifik. Primer tersebut dirancang berdasarkansekuen DNA dari gen target asal spesiesorganisme yang sama dengan yang digunakandalam penelitian. RT-PCR dengan primertersebut menghasilkan fragmen DNA yangukurannya sesuai dengan gen lengkap penyandiβ-1,6-glukanase, yaitu sekitar 1300 bp. Setelahsecara berurutan diklon menggunakan vektorpGEM-T Easy, sekuensing urutan DNA dananalisis BlastN maupun BlastX dari sekuen yangdiperoleh, terbukti bahwa fragmen DNA tersebutadalah gen lengkap penyandi β-1,6-glukanase.Hal ini ditunjukkan oleh Nilai Kesamaan(Identity) dan E-Value yang masing-masingmencapai 96% dan 0.0.

show abstract

“…There are many biomacromolecules and surface charges on the cell wall. They can interact with metal ions or charged ionic groups, provoke the deposition of mineral, provide oriented nucleation sites, immobilize the particles, and finally establish microsphere structure [44].…”

Section: Formation Mechanism Of Lvp/lfp-chmsmentioning

confidence: 99%

Li 3 V 2 (PO 4 ) 3 /LiFePO 4 composite hollow microspheres for wide voltage lithium ion batteries

Wei

Zhang

et al. 2016

Electrochimica Acta

View full text Add to dashboard Cite

, Li3V2(PO4)3/LiFePO4 composite hollow microspheres for wide voltage lithium ion batteries, Electrochimica Acta http://dx.doi.org/10.1016/j.electacta. 2016.10.047 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.  The cathode exhibits a higher discharge capacity and energy density. Its capacity is far higher than the capacity of unsubstituted LFP and LVP in the same wide voltage range. The energy density of this cell is about 4 times higher than that of modern commercial lithium-ion batteries (157 Wh kg -1 ). The wide voltage range not only increases the discharge capacity and energy density of cathode materials, but also could expand the range of its applications in electronic equipment.3

show abstract

Progress in Metabolic Engineering of Saccharomyces cerevisiae

Cited by 526 publications

References 416 publications

Combined production of biogas and ethanol at high solids loading from wheat straw impregnated with acetic acid: experimental study and techno-economic evaluation

Combined production of biogas and ethanol at high solids loading from wheat straw impregnated with acetic acid: experimental study and techno-economic evaluation

Kloning gen penyandi β-1,6-glukanase kapang secara cepat dengan teknik RT-PCR menggunakan primer spesifik Rapid cloning for gene encoding fungal β-1,6-glucanase by means of RT-PCR using specific primers

Li 3 V 2 (PO 4 ) 3 /LiFePO 4 composite hollow microspheres for wide voltage lithium ion batteries

Contact Info

Product

Resources

About