Progress in infrared spectroscopy as an efficient tool for predicting protein secondary structure

Yang, Shouning; Zhang, Qiaoling; Yang, Huayan; Shi, Haimei; Dong, Aichun; Wang, Li; Yu, Shaoning

doi:10.1016/j.ijbiomac.2022.02.104

Cited by 92 publications

(55 citation statements)

References 209 publications

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“…As such, Figures 2 and 3 present the infrared absorption spectra of the dental biofilm before prophylaxis (stage I), after toothpaste (stage II), and after calcium glycerophosphate mineral complex (stage III), averaged over the groups participating in the study. Analysis of the obtained spectra, based on studies in which IR spectroscopy was used to study oral biological fluids, biofilms, human dental hard tissues, and phosphates relevant to enamel and dentin formation [26,28,29,[41][42][43][44][45][46][47], showed that the experimental IR spectra of biofilms have a typical set of basic vibrations.…”

Section: Resultsmentioning

confidence: 99%

“…The main absorption bands present in all IR spectra of biofilm samples can be associated with the following molecular groups. Analysis of the obtained spectra, based on studies in which IR spectroscopy was used to study oral biological fluids, biofilms, human dental hard tissues, and phosphates relevant to enamel and dentin formation [26,28,29,[41][42][43][44][45][46][47], showed that the experimental IR spectra of biofilms have a typical set of basic vibrations.…”

Section: Resultsmentioning

confidence: 99%

“…Analysis of the obtained spectra, based on studies in which IR spectroscopy was used to study oral biological fluids, biofilms, human dental hard tissues, and phosphates relevant to enamel and dentin formation [ 26 , 28 , 29 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ], showed that the experimental IR spectra of biofilms have a typical set of basic vibrations.…”

Section: Resultsmentioning

confidence: 99%

“…A band of IR spectra from 1850 to 1350 cm −1 includes a band in the region of 1730 cm −1 that can be attributed to (>C=O) phospholipids, esters, fatty acids [ 28 , 29 , 43 , 48 ], and the characteristic region of proteins. Protein bands include Amide I (C=O stretch) vibrations in the region from 1675–1615 cm −1 , Amide II (60% N–H bend and 40% C–N stretch) vibrations in the region from 1575–1520 cm −1 , and Amide III (40% C–N stretch, 30% N–H bend) vibrations in the region from 1315–1270 cm −1 [ 28 , 29 , 41 , 42 , 43 , 45 ]. For dental biofilm, these vibrations can also be correlated with peptides [ 28 , 43 ].…”

Section: Resultsmentioning

confidence: 99%

“…For dental biofilm, these vibrations can also be correlated with peptides [ 28 , 43 ]. The distinguished group of bands in the region from 1480 to 1350 cm −1 correlate with vibrations of CH 2 /CH 3 groups of proteins and lipids [ 28 , 29 , 41 , 45 ]. The band at 1394 cm −1 represents a C=O symmetric stretch of the COO- group and is related to biofilm lipids [ 28 , 29 , 41 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

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Effect of Exo/Endogenous Prophylaxis Dentifrice/Drug and Cariogenic Conditions of Patient on Molecular Property of Dental Biofilm: Synchrotron FTIR Spectroscopic Study

et al. 2022

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(1) Objectives: This study is the first one to investigate the molecular composition of the dental biofilm during the exogenous and endogenous prophylaxis stages (use of dentifrice/drug) of individuals with different cariogenic conditions using molecular spectroscopy methods. (2) Materials and Methods: The study involved 100 participants (50 males and 50 females), aged 18–25 years with different caries conditions. Biofilm samples were collected from the teeth surface of all participants. The molecular composition of biofilms was investigated using synchrotron infrared microspectroscopy. Changes in the molecular composition were studied through calculation and analysis of ratios between organic and mineral components of biofilm samples. (3) Results: Based on the data obtained by synchrotron FTIR, calculations of organic and mineral component ratios, and statistical analysis of the data, we were able to assess changes occurring in the molecular composition of the dental biofilm. Variations in the phosphate/protein/lipid, phosphate/mineral, and phospholipid/lipid ratios and the presence of statistically significant intra- and inter-group differences in these ratios indicate that the mechanisms of ion adsorption, compounds and complexes arriving from oral fluid into dental biofilm during exo/endogenous prophylaxis, differ for patients in norm and caries development. (4) Conclusions: The conformational environment and charge interaction in the microbiota and the electrostatic state of the biofilm protein network in patients with different cariogenic conditions play an important role. (5) Clinical Significance: Understanding the changes that occur in the molecular composition of the dental biofilm in different oral homeostasis conditions will enable successful transition to a personalised approach in dentistry and high-tech healthcare.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Exo/Endogenous Prophylaxis Dentifrice/Drug and Cariogenic Conditions of Patient on Molecular Property of Dental Biofilm: Synchrotron FTIR Spectroscopic Study

et al. 2022

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Determination of process parameters and precipitation methods for potential large‐scale production of sugar beet leaf protein concentrate

Akyüz,

Tekin,

Aksoy

et al. 2023

J Sci Food Agric

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BACKGROUNDSugar beet is one of the most produced industrial plants in the world, and during manufacturing it produces a large quantity of leaf waste. Because this waste is rich in protein, this study aimed to identify an efficient method for producing large‐scale protein concentrate from sugar beet leaves.RESULTSResults showed that protein extraction from fresh leaves was more effective than from dried leaves. Maximum protein extraction was achieved at pH 9, compared with pH 7 or 8. Blanching as a pretreatment reduced protein yield during isoelectric precipitation, with a yield of 2.31% compared to 20.20% without blanching. Consequently, blanching was excluded from the extraction process. After extraction, isoelectric precipitation, heat coagulation, and isoelectric–ammonium sulfate precipitation were compared. Although the latter resulted in the highest protein yield, Fourier transform infrared analysis revealed that excessive salt was not removed during dialysis, making it unsuitable for scale‐up due to its additional cost and complexity. Therefore, isoelectric precipitation was selected as the appropriate method for protein precipitation from sugar beet leaves. To increase yield, extractions were assisted by ultrasound or enzyme addition. Ultrasound‐assisted extraction resulted in an increased protein yield from 20.20% to 28.60%, while Pectinex Ultra SP‐L‐assisted extraction was the most effective, increasing protein yield from 20.20% to 38.09%.CONCLUSIONProteins were extracted from fresh sugar beet leaves using optimum conditions (50 °C, 30 min, pH 9) and precipitated at isoelectric point, with enzymatic‐assisted extraction yielding the maximum protein recovery. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Secondary Structures in Synthetic Poly(Amino Acids): Homo‐ and Copolymers of Poly(Aib), Poly(Glu), and Poly(Asp)

Rohmer¹,

Freudenberg²,

Binder³

2023

Macromolecular Bioscience

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The secondary structure of poly(amino acids) is an excellent tool for controlling and understanding the functionality and properties of proteins. In this perspective article the secondary structures of the homopolymers of oligo‐ and poly‐glutamic acid (Glu), aspartic acid (Asp), and α‐aminoisobutyric acid (Aib) are discussed. Information on external and internal factors, such as the nature of side groups, interactions with solvents and interactions between chains is reviewed. A special focus is directed on the folding in hybrid‐polymers consisting of oligo(amino acids) and synthetic polymers. Being part of the SFB TRR 102 “Polymers under multiple constraints: restricted and controlled molecular order and mobility” this overview is embedded into the cross section of protein fibrillation and supramolecular polymers. As polymer‐ and amino acid folding is an important step for the utilization and design of future biomolecules these principles guide to a deeper understanding of amyloid fibrillation.

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Progress in infrared spectroscopy as an efficient tool for predicting protein secondary structure

Cited by 92 publications

References 209 publications

Effect of Exo/Endogenous Prophylaxis Dentifrice/Drug and Cariogenic Conditions of Patient on Molecular Property of Dental Biofilm: Synchrotron FTIR Spectroscopic Study

Effect of Exo/Endogenous Prophylaxis Dentifrice/Drug and Cariogenic Conditions of Patient on Molecular Property of Dental Biofilm: Synchrotron FTIR Spectroscopic Study

Determination of process parameters and precipitation methods for potential large‐scale production of sugar beet leaf protein concentrate

Secondary Structures in Synthetic Poly(Amino Acids): Homo‐ and Copolymers of Poly(Aib), Poly(Glu), and Poly(Asp)

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