Progress in bacterial cellulose matrices for biotechnological applications

Cacicedo, Maximiliano L.; Castro, M. Cristina; Servetas, Ioannis; Bosnea, Loulouda; Boura, Konstantina; Tsafrakidou, Panagiota; Dima, Agapi; Τέρπου, Αντωνία; Κουτίνας, Αθανάσιος Α.; Castro, Guillermo R.

doi:10.1016/j.biortech.2016.02.071

Cited by 248 publications

(162 citation statements)

References 67 publications

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“…This can be accomplished through the modification of BC. There is a great diversity of modifications that can be applied to BC, which can be grouped in two distinct classes: in situ and ex situ modifications, consisting respectively in the incorporation of exogenous molecules that are added to the culture medium during BC biosynthesis, or performed after the BC is produced and purified, and can be carried out either by chemical or physical methods . Porosity is a great concern in applications, such as the development of scaffolds, for cell growth.…”

Section: Introductionmentioning

confidence: 99%

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

Carvalho

Guedes

Sousa

et al. 2019

Biotechnology Journal

120

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Bacterial cellulose (BC) is a nanocellulose form produced by some nonpathogenic bacteria. BC presents unique physical, chemical, and biological properties that make it a very versatile material and has found application in several fields, namely in food industry, cosmetics, and biomedicine. This review overviews the latest state‐of‐the‐art usage of BC on three important areas of the biomedical field, namely delivery systems, wound dressing and healing materials, and tissue engineering for regenerative medicine. BC will be reviewed as a promising biopolymer for the design and development of innovative materials for the mentioned applications. Overall, BC is shown to be an effective and versatile carrier for delivery systems, a safe and multicustomizable patch or graft for wound dressing and healing applications, and a material that can be further tuned to better adjust for each tissue engineering application, by using different methods.

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Section: Introductionmentioning

confidence: 99%

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

Carvalho

Guedes

Sousa

et al. 2019

Biotechnology Journal

120

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“…Ultrapure bionanocellulose (BNC) is finding applications in biotechnology, medicine, and various industry sectors. In comparison to plant-derived cellulose, Komagataeibacter produces BNC with superior mechanical strength, purity, water-holding capacity, and biodegradability (Cacicedo et al, 2016;Gama, Dourado, & Bielecki, 2016). Cellulose, a water-insoluble exopolysaccharide composed of β-1,4-glucan chains, gives the bacterial cells protection from UV radiation or desiccation (Williams & Cannon, 1989).…”

mentioning

confidence: 99%

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Ryngajłło

Kubiak

Jędrzejczak-Krzepkowska

et al. 2018

MicrobiologyOpen

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Komagataeibacter species are well‐recognized bionanocellulose ( BNC ) producers. This bacterial genus, formerly assigned to Gluconacetobacter , is known for its phenotypic diversity manifested by strain‐dependent carbon source preference, BNC production rate, pellicle structure, and strain stability. Here, we performed a comparative study of nineteen Komagataeibacter genomes, three of which were newly contributed in this work. We defined the core genome of the genus, clarified phylogenetic relationships among strains, and provided genetic evidence for the distinction between the two major clades, the K . xylinus and the K. hansenii . We found genomic traits, which likely contribute to the phenotypic diversity between the Komagataeibacter strains. These features include genome flexibility, carbohydrate uptake and regulation of its metabolism, exopolysaccharides synthesis, and the c‐di‐ GMP signaling network. In addition, this work provides a comprehensive functional annotation of carbohydrate metabolism pathways, such as those related to glucose, glycerol, acetan, levan, and cellulose. Findings of this multi‐genomic study expand understanding of the genetic variation within the Komagataeibacter genus and facilitate exploiting of its full potential for bionanocellulose production at the industrial scale.

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“…Although the process of bacterial cellulose biosynthesis has not been completely elucidated, studies involving microorganisms from genus Komagataeibacter have demonstrated the production of bacterial cellulose with distinct characteristics (crystallinity, thickness, tensile strength, among others), depending on growth conditions such as the carbon and nitrogen sources, media additives, etc. [51, 52, 53]. This fact suggests that different Bcs operons may be activated upon changes in environmental factors, producing cellulose fibrils with different structural properties.…”

Section: Resultsmentioning

confidence: 99%

Structure and function of a novel GH8 endoglucanase from the bacterial cellulose synthase complex of Raoultella ornithinolytica

et al. 2017

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Cellulose synthesis in bacteria is a complex process involving the concerted action of several enzymes whose genes are often organized in operons. This process influences many fundamental physiological aspects such as bacteria and host interaction, biofilm formation, among others. Although it might sound contradictory, the participation of cellulose-degrading enzymes is critical to this process. The presence of endoglucanases from family 8 of glycosyl hydrolases (GH8) in bacterial cellulose synthase (Bcs) complex has been described in different bacteria, including the model organism Komagataeibacter xylinus; however, their role in this process is not completely understood. In this study, we describe the biochemical characterization and three-dimensional structure of a novel GH8 member from Raoultella ornithinolytica, named AfmE1, which was previously identified by our group from the metagenomic analysis of the giant snail Achatina fulica. Our results demonstrated that AfmE1 is an endo-β-1,4-glucanase, with maximum activity in acidic to neutral pH over a wide temperature range. This enzyme cleaves cello-oligosaccharides with a degree of polymerization ≥ 5 and presents six glucosyl-binding subsites. The structural comparison of AfmE1 with other GH8 endoglucanases showed significant structural dissimilarities in the catalytic cleft, particularly in the subsite +3, which correlate with different functional mechanisms, such as the recognition of substrate molecules having different arrangements and crystallinities. Together, these findings provide new insights into molecular and structural features of evolutionarily conserved endoglucanases from the bacterial cellulose biosynthetic machinery.

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Progress in bacterial cellulose matrices for biotechnological applications

Cited by 248 publications

References 67 publications

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Structure and function of a novel GH8 endoglucanase from the bacterial cellulose synthase complex of Raoultella ornithinolytica

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