Progress in analytical imaging of the cell by dynamic secondary ion mass spectrometry (SIMS microscopy)

Guerquin‐Kern, Jean‐Luc; Wu, Ting-Di; Quintana, Carmen; Croisy, Alain

doi:10.1016/j.bbagen.2005.05.013

Cited by 238 publications

(189 citation statements)

References 17 publications

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“…SIMS imaging was performed using a NanoSIMS-50 Ion Microprobe (CAMECA) operating in scanning mode (32,33). The elemental mapping was performed on the same thin section previously observed by TEM.…”

Section: Methodsmentioning

confidence: 99%

Desulfovibrio magneticus RS-1 contains an iron- and phosphorus-rich organelle distinct from its bullet-shaped magnetosomes

Byrne

Ball²,

Guerquin‐Kern³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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107

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Intracellular magnetite crystal formation by magnetotactic bacteria has emerged as a powerful model for investigating the cellular and molecular mechanisms of biomineralization, a process common to all branches of life. Although magnetotactic bacteria are phylogenetically diverse and their crystals morphologically diverse, studies to date have focused on a few, closely related species with similar crystal habits. Here, we investigate the process of magnetite biomineralization in Desulfovibrio magneticus sp. RS-1, the only reported species of cultured magnetotactic bacteria that is outside of the α-Proteobacteria and that forms bulletshaped crystals. Using a variety of high-resolution imaging and analytical tools, we show that RS-1 cells form amorphous, noncrystalline granules containing iron and phosphorus before forming magnetite crystals. Using NanoSIMS (dynamic secondary ion mass spectroscopy), we show that the iron-phosphorus granules and the magnetite crystals are likely formed through separate cellular processes. Analysis of the cellular ultrastructure of RS-1 using cryo-ultramicrotomy, cryo-electron tomography, and tomography of ultrathin sections reveals that the magnetite crystals are not surrounded by membranes but that the iron-phosphorus granules are surrounded by membranous compartments. The varied cellular paths for the formation of these two minerals lead us to suggest that the iron-phosphorus granules constitute a distinct bacterial organelle.bacterial organelle | biomineralization | magnetotactic bacteria | dynamic secondary ion mass spectroscopy | magnetite B iomineralization, the biologically controlled transformation of inorganic compounds into highly-ordered structures, is performed by organisms in all branches of life, from bacteria to humans. Because biogenic minerals often have superior properties to their chemically synthesized counterparts, understanding biomineralization is important to fields as diverse as inorganic materials synthesis and medicine (1, 2). Among the numerous organisms capable of biomineralization, magnetotactic bacteria (MB), a group of microbes that form intracellular chains of magnetic minerals including magnetite and greigite, have become powerful models for studying biomineralization because of their relatively fast growth rate and genetic tractability (3).Investigation of the cellular ultrastructure of MB has shown that the magnetite crystals are formed within membranous intracellular compartments called magnetosomes, which are present before the formation of magnetite (4, 5). Other studies have shown that when magnetite formation is induced by decreasing oxygen levels or adding iron to iron-deprived cells, multiple crystals in the chain nucleate simultaneously (5, 6). These insights into the kinetics of magnetite biomineralization have been complemented by molecular studies. A genomic island called the magnetosome island (MAI) has been found in all MB studied to date. Loss of the MAI results in an absence of magnetosome membranes and magnetite crystals, and gene...

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Section: Methodsmentioning

confidence: 99%

Desulfovibrio magneticus RS-1 contains an iron- and phosphorus-rich organelle distinct from its bullet-shaped magnetosomes

Byrne

Ball²,

Guerquin‐Kern³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

107

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“…In the past several years, there has been an explosion in the number of researchers utilizing imaging mass spectrometric techniques for biological applications [1][2][3][4][5][6]. While many of these have focused on the analysis of tissue samples, there is a clear need for analysis on a single-cell level as well.…”

Section: Introductionmentioning

confidence: 99%

“…Secondary ion mass spectrometry (SIMS), in both dynamic and static mode, has been widely applied to imaging analysis of single cells. Recent advances in dynamic SIMS imaging of cells have been reviewed elsewhere [4], with many examples showing excellent spatial resolution and localization of elemental species within cells. Very recently, the newest generation of dynamic SIMS instrumentation, NanoSIMS, has been used to obtain subcellular localization of a peptide vector [11], study diatom cell division [12], and perform nanoautography with stable isotope tracers [13].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Single Cells for Imaging Mass Spectrometry

Berman¹,

Fortson²,

Kulp

2010

Methods in Molecular Biology

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Characterizing chemical changes within single cells is important for determining fundamental mechanisms of biological processes that will lead to new biological insights and improved disease understanding. Imaging biological systems with mass spectrometry (MS) has gained popularity in recent years as a method for creating precise chemical maps of biological samples.In order to obtain high-quality mass spectral images that provide relevant molecular information about individual cells, samples must be prepared so that salts and other cell-culture components are removed from the cell surface and the cell contents are rendered accessible to the desorption beam. We have designed a cellular preparation protocol for imaging MS that preserves the cellular contents for investigation and removes the majority of the interfering species from the extracellular matrix. Using this method, we obtain excellent imaging results and reproducibility in three diverse cell types: MCF7 human breast cancer cells, Madin-Darby canine kidney (MDCK) cells, and NIH/3T3 mouse fibroblasts. This preparation technique allows routine imaging MS analysis of cultured cells, allowing for any number of experiments aimed at furthering scientific understanding of molecular processes within individual cells.

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“…In this case, the primary beam path is strongly modified to become coaxial with the secondary beam within the objective column, and this configuration imposes the use of primary ions of sign opposite to those of the observed secondary ions. For example, CAMECA NanoSIMS 50 equipment has demonstrated a high lateral resolution (150 nm or less and 50 nm or less with primary O 2 + and Cs + ions, respectively), the ability to detect simultaneously five different ions from the same microvolume and a very good transmission even at high mass resolution [82]. During the last few years, nanoSIMS technology has been strongly investigated for a wide range of applications, including isotopic measurements of presolar silicon carbide grains from supernovae [83], for imaging of As traces in human hair [84], to test the toxic effects of Al, Co and Cr particles in cells [82], to study the localisation of Fe in Alzheimer's disease hippocampus [85] and for surface chemical analysis of DNA microarrays [79].…”

Section: Secondary Ion Mass Spectrometry and Sputtered Neutral Mass Smentioning

confidence: 99%

Inorganic mass spectrometry as a tool for characterisation at the nanoscale

et al. 2009

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Inorganic mass spectrometry techniques may offer great potential for the characterisation at the nanoscale, because they provide unique elemental information of great value for a better understanding of processes occurring at nanometre-length dimensions. Two main groups of techniques are reviewed: those allowing direct solid analysis with spatial resolution capabilities, i.e. lateral (imaging) and/or indepth profile, and those for the analysis of liquids containing colloids. In this context, the present capabilities of widespread elemental mass spectrometry techniques such as laser ablation coupled with inductively coupled plasma mass spectrometry (ICP-MS), glow discharge mass spectrometry and secondary ion/neutral mass spectrometry are described and compared through selected examples from various scientific fields. On the other hand, approaches for the characterisation (i.e. size, composition, presence of impurities, etc.) of colloidal solutions containing nanoparticles by the well-established ICP-MS technique are described. In this latter case, the capabilities derived from the on-line coupling of separation techniques such as field-flow fractionation and liquid chromatography with ICP-MS are also assessed. Finally, appealing trends using ICP-MS for bioassays with biomolecules labelled with nanoparticles are delineated.

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Progress in analytical imaging of the cell by dynamic secondary ion mass spectrometry (SIMS microscopy)

Cited by 238 publications

References 17 publications

Desulfovibrio magneticus RS-1 contains an iron- and phosphorus-rich organelle distinct from its bullet-shaped magnetosomes

Desulfovibrio magneticus RS-1 contains an iron- and phosphorus-rich organelle distinct from its bullet-shaped magnetosomes

Preparation of Single Cells for Imaging Mass Spectrometry

Inorganic mass spectrometry as a tool for characterisation at the nanoscale

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