Progress in Adzuki Bean Seed Coat Colour Studies

Wang, Zhen; Zhao, Wei; Huang, Yufei; Zhao, Pu; Yang, Kai; Wan, Ping; Chu, Liwei

doi:10.3390/plants12183242

Cited by 1 publication

(3 citation statements)

References 41 publications

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“…In the accessions with black seeds, Vigun05g039500 sequences were very conservative, so this gene was present in two studied black-seeded accessions. These results were consistent with the data obtained earlier [34]. PCR products with different primers to Vigun05g039500 were not observed in some non-black accessions (one white-seeded anthocyanin-free control, accessions with light-brown and brown seeds lacking anthocyanins, and one reddish-brown-seeded accession with low anthocyanin content).…”

Section: Discussionsupporting

confidence: 92%

“…Candidate genes, acting as regulatory genes of the MBW complex, were suggested using different methods [34,35]. The VuCPC (Vigun11g115400) and VuMYB4 (Vigun01g142900) genes were identified in the cowpea genome.…”

Section: Discussionmentioning

confidence: 99%

“…Cultivars with colored seeds, pods, and sprouts have a specific dietary value [28][29][30][31][32]. Colored seeds in the legume family are rich in phenolic components that have an antioxidant effect due to their ability to trap free radicals and exhibit antimutagenic and anticarcinogenic activities [29,30,33,34]. The anticarcinogenic effect of phenolic compounds in V. unguiculata can manifest itself in the form of protecting DNA from damage and in suppressing the proliferation of cancer cells [6,35].…”

Section: Introductionmentioning

confidence: 99%

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The Content of Anthocyanins in Cowpea (Vigna unguiculata (L.) Walp.) Seeds and Contribution of the MYB Gene Cluster to Their Coloration Pattern

Krylova,

Mikhailova,

Zinchenko

et al. 2023

Plants

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The intensively pigmented legumes belonging to Phaseolus and Vigna spp. are valued as an essential component of healthy nutrition due to their high content of flavonoids. In this context, we used the accessions of Vigna unguiculata with different colors of seed coats from the N.I. Vavilov All-Russian Institute of Plant Genetic Resources collection as the main object of this research. We applied confocal laser scanning microscopy, biochemical analysis, and wide in silico and molecular genetic analyses to study the main candidate genes for anthocyanin pigmentation within the MYB cluster on chromosome 5. We performed statistical data processing. The anthocyanin content ranged from 2.96 mg/100 g DW in reddish-brown-seeded cowpea accessions to 175.16 mg/100 g DW in black-seeded ones. Laser microscopy showed that the autofluorescence in cowpea seeds was mainly caused by phenolic compounds. The maximum fluorescence was observed in the seed coat, while its dark color, due to the highest level of red fluorescence, pointed to the presence of anthocyanins and anthocyanidins. Genes of the MYB cluster on chromosome 5 demonstrated a high homology and were segregated into a separate clade. However, amplification products were not obtained for all genes because of the truncation of some genes. Statistical analysis showed a clear correlation between the high content of anthocyanins in cowpea seeds and the presence of PCR products with primers Vigun05g0393-300-1.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%